Click here to switch to the map view.

The map label for this gene is dhbF [H]

Identifier: 34498254

GI number: 34498254

Start: 3033775

End: 3035094

Strand: Reverse

Name: dhbF [H]

Synonym: CV_2799

Alternate gene names: 34498254

Gene position: 3035094-3033775 (Counterclockwise)

Preceding gene: 34498255

Following gene: 34498253

Centisome position: 63.88

GC content: 63.71

Gene sequence:

>1320_bases
ATGACGGTGAAGCGATGGATGGTGCAGGATAGCGTGGGCAGCGGAGCGGCGCTGGCGAGTCTCGAAGGCGAGGAGACTGG
CCGGCTTTGGCTAGCGGCCCAGCCGGAGCATGGATTCCAGACCTTGCAAGGCTTGGCTTATGCGCTGGCGCGCGAGGTGC
GCCGTTTGCAGCCCGATAGCCCTTATCGGCTGGCGGGAAAAGGCGTTCTGGGCGCGCTGGCTTATGAAGTGGGCTTGCAG
TTGCGAGGCATGGGGCAGACTGTAGCCTGCGTTGCGCTGGAGGGCGCGCCGGCATGGGTTCGACAGTCCGAAGCGCTGGC
GGTCGAGCGGTCGCAAGCCAGTTTGCCGGCATGGTTGCGCCGCTGCCTTTCATCCTATGTCCCCGAACCGGGCGATTTTC
CATTGCAGCCAACGAATATGCCGGCATCCGCCGAGGCTTTCTGGCGCGCTCTGGGCGCGCAAGCGAGCGCGCGAGTCCCT
GCTCACCAGCCGCTGGTGATGCTCAGGCAGGGACGGCAATCGTTGCCGCCATTGATTTGCGTGCCGGGCGCGGGTGCCAG
CGTGGTGGATTTCTTCGCGCTTTCTAGCGCGCTTGCCTATCAAGGGAGCGTGTATGGGCTGCAGCCAAGGGGGCTGGATG
GCAGACAGCCTCCGCATGTGACGGTCGAGGATGCGGCGCGGTCGTATCTGGACGCGCTGCGGGCGAACGCGCCAGGGCCA
GTGCATTTGCTTGGGCATTCCTTCGGCGGCTGGATCGCGCATGAGCTGGCGGCGCAATTGCAAAGCGCGGGAACCCGCGT
GTTGTCGTTGACCGTGCTGGACAGCCGGGTGCCGAATGATGCGCCAGGCGAGTATGGCCAGCGCGAAGTGCTGAGCAGGC
TGATTGCCTTATATCAGCAGGGTTGCGGAGAGCCGCTCAGACTCGATGCGGCGGAATTGGCGGAGCGAAGCGCGGAGGAG
CAAAGAAAGGCTTTGCATGGCGAATTGCTCAGAGTCGGCATTCTGCCTTCTCGCAGCTCCCCGGATATCTTGAGCGGGGT
GCTCAGAACCTTTGCTTGCGCTTTGCGAGCCGGATACCGGCCAGAGCGCTTGTATCAAGGCCCCAGCCGGCTGGTGCTGG
TCAAACCGTCGGAAGCGCGTTCGTCAGAAGAGGCGACGTCGCGCGAGCAATGGATTGCGGGTTGGCGCAGATGGCTTCCC
GACTTGGTGTTCTGGGAGGGGCCGGGCAATCACATCACCTTGTTGCGGTCGCCTCAGGCTGGGCACTTGGCGGCATGGTT
GGACGAAATGCTGTTCGACATGAAAGCGGTGCACTCGTGA

Upstream 100 bases:

>100_bases
CCGGATTCGCGACTCAGCGATTGCCGGCGGAAAACTAGGCATGGCTTGTCCATGATGGGCTGAATGGCGGCGATTTATCT
GCCAATAGGATGAAAGGCTG

Downstream 100 bases:

>100_bases
GCTTGGTGCTGAAGCGATTTCGATCCTTGGGCGCCGCGTTTGCTTGCATGTGGCTGAGCGAGATGATTACGGGCTTGGGC
AGTCTATTGCTGCAATTCGC

Product: hypothetical protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTVKRWMVQDSVGSGAALASLEGEETGRLWLAAQPEHGFQTLQGLAYALAREVRRLQPDSPYRLAGKGVLGALAYEVGLQ
LRGMGQTVACVALEGAPAWVRQSEALAVERSQASLPAWLRRCLSSYVPEPGDFPLQPTNMPASAEAFWRALGAQASARVP
AHQPLVMLRQGRQSLPPLICVPGAGASVVDFFALSSALAYQGSVYGLQPRGLDGRQPPHVTVEDAARSYLDALRANAPGP
VHLLGHSFGGWIAHELAAQLQSAGTRVLSLTVLDSRVPNDAPGEYGQREVLSRLIALYQQGCGEPLRLDAAELAERSAEE
QRKALHGELLRVGILPSRSSPDILSGVLRTFACALRAGYRPERLYQGPSRLVLVKPSEARSSEEATSREQWIAGWRRWLP
DLVFWEGPGNHITLLRSPQAGHLAAWLDEMLFDMKAVHS

Sequences:

>Translated_439_residues
MTVKRWMVQDSVGSGAALASLEGEETGRLWLAAQPEHGFQTLQGLAYALAREVRRLQPDSPYRLAGKGVLGALAYEVGLQ
LRGMGQTVACVALEGAPAWVRQSEALAVERSQASLPAWLRRCLSSYVPEPGDFPLQPTNMPASAEAFWRALGAQASARVP
AHQPLVMLRQGRQSLPPLICVPGAGASVVDFFALSSALAYQGSVYGLQPRGLDGRQPPHVTVEDAARSYLDALRANAPGP
VHLLGHSFGGWIAHELAAQLQSAGTRVLSLTVLDSRVPNDAPGEYGQREVLSRLIALYQQGCGEPLRLDAAELAERSAEE
QRKALHGELLRVGILPSRSSPDILSGVLRTFACALRAGYRPERLYQGPSRLVLVKPSEARSSEEATSREQWIAGWRRWLP
DLVFWEGPGNHITLLRSPQAGHLAAWLDEMLFDMKAVHS
>Mature_438_residues
TVKRWMVQDSVGSGAALASLEGEETGRLWLAAQPEHGFQTLQGLAYALAREVRRLQPDSPYRLAGKGVLGALAYEVGLQL
RGMGQTVACVALEGAPAWVRQSEALAVERSQASLPAWLRRCLSSYVPEPGDFPLQPTNMPASAEAFWRALGAQASARVPA
HQPLVMLRQGRQSLPPLICVPGAGASVVDFFALSSALAYQGSVYGLQPRGLDGRQPPHVTVEDAARSYLDALRANAPGPV
HLLGHSFGGWIAHELAAQLQSAGTRVLSLTVLDSRVPNDAPGEYGQREVLSRLIALYQQGCGEPLRLDAAELAERSAEEQ
RKALHGELLRVGILPSRSSPDILSGVLRTFACALRAGYRPERLYQGPSRLVLVKPSEARSSEEATSREQWIAGWRRWLPD
LVFWEGPGNHITLLRSPQAGHLAAWLDEMLFDMKAVHS

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG3319

COG function: function code Q; Thioesterase domains of type I polyketide synthases or non-ribosomal peptide synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 47626; Mature: 47495

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKRWMVQDSVGSGAALASLEGEETGRLWLAAQPEHGFQTLQGLAYALAREVRRLQPDS
CCCHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
PYRLAGKGVLGALAYEVGLQLRGMGQTVACVALEGAPAWVRQSEALAVERSQASLPAWLR
CCEECCCHHHHHHHHHHCCCEECCCCEEEEEEECCCCHHHHCCHHHHHHHHHCCHHHHHH
RCLSSYVPEPGDFPLQPTNMPASAEAFWRALGAQASARVPAHQPLVMLRQGRQSLPPLIC
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHCCCEEE
VPGAGASVVDFFALSSALAYQGSVYGLQPRGLDGRQPPHVTVEDAARSYLDALRANAPGP
ECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCC
VHLLGHSFGGWIAHELAAQLQSAGTRVLSLTVLDSRVPNDAPGEYGQREVLSRLIALYQQ
EEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
GCGEPLRLDAAELAERSAEEQRKALHGELLRVGILPSRSSPDILSGVLRTFACALRAGYR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
PERLYQGPSRLVLVKPSEARSSEEATSREQWIAGWRRWLPDLVFWEGPGNHITLLRSPQA
HHHHHCCCCCEEEECCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCC
GHLAAWLDEMLFDMKAVHS
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVKRWMVQDSVGSGAALASLEGEETGRLWLAAQPEHGFQTLQGLAYALAREVRRLQPDS
CCHHHHHHCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
PYRLAGKGVLGALAYEVGLQLRGMGQTVACVALEGAPAWVRQSEALAVERSQASLPAWLR
CCEECCCHHHHHHHHHHCCCEECCCCEEEEEEECCCCHHHHCCHHHHHHHHHCCHHHHHH
RCLSSYVPEPGDFPLQPTNMPASAEAFWRALGAQASARVPAHQPLVMLRQGRQSLPPLIC
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCHHHCCCEEE
VPGAGASVVDFFALSSALAYQGSVYGLQPRGLDGRQPPHVTVEDAARSYLDALRANAPGP
ECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCC
VHLLGHSFGGWIAHELAAQLQSAGTRVLSLTVLDSRVPNDAPGEYGQREVLSRLIALYQQ
EEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
GCGEPLRLDAAELAERSAEEQRKALHGELLRVGILPSRSSPDILSGVLRTFACALRAGYR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
PERLYQGPSRLVLVKPSEARSSEEATSREQWIAGWRRWLPDLVFWEGPGNHITLLRSPQA
HHHHHCCCCCEEEECCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCC
GHLAAWLDEMLFDMKAVHS
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]