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The map label for this gene is leuB

Identifier: 34498233

GI number: 34498233

Start: 3014383

End: 3015447

Strand: Reverse

Name: leuB

Synonym: CV_2778

Alternate gene names: 34498233

Gene position: 3015447-3014383 (Counterclockwise)

Preceding gene: 34498234

Following gene: 34498232

Centisome position: 63.47

GC content: 66.57

Gene sequence:

>1065_bases
ATGAAGATCGCCATTCTGCCGGGAGACGGCATCGGCCCCGAAATCATCGCCCAGGCCGAACGCGTGCTGGAGGTGCTGCG
TCGCGACGGTCTGAAGATCGAAACCGAGCATGCGCCGCTGGGCGGCGCCGCTTACGACCAGTACGGCGTGCCGTATCCCG
AGGCCACGCAGAAACTGGCGCGCGAAGCCGACGCCGTGCTGCTGGGCGCGGTGGGCGGCCCCGCCTACGACAAGCTGGAC
CGTCCGCTGCGCCCGGAGCGCGGCCTGCTGGCGATCCGCAAGGACCTCAACCTGTTCGCCAACCTGCGGCCGGCCATCCT
GTATCCGGAGCTGGCCAACGCCTCGACGCTGAAGCCGGAAGTGGTGGCCGGGCTGGACATCATGATCGTCCGCGAGCTGA
CCGGCGACATCTACTTCGGCCAGCCGCGCGGCATCGCCGTCAACGAGCTGGGCGAGCGCGAGGCCTACAACACCATGCGC
TACAGCGAGAGCGAAGTGCGCCGCATCGCCCACGTGGCCTTCGGCATCGCCATGAAGCGCAACCGCAAGCTGTGCTCGGT
GGACAAGGCCAATGTGCTGGAAACCACCGAATTCTGGAAGGAAATCATGATCGAGGTGGCCAAGGAGTATCCGCAAGTGG
AGCTCAGCCACATGTACGTCGACAACGCCGCGATGCAGCTGGTGCGCAACCCGAAGCAGTTCGACGTGATGGTCACCGGC
AACATCTTCGGCGACATCCTGTCCGACGAGGCCTCGATGCTGACCGGCTCCATCGGCATGCTGCCGTCCGCCTCGCTGGA
TCAGAACAACAAGGGCCTGTACGAGCCCAGCCACGGCTCGGCGCCGGACATCGCCGGCCAGAACCTGGCCAACCCGCTGG
CCACCATCCTGTCCGCCGCGATGATGCTGCGCTACAGCTTCGGCCAGGAAGCCGCCGCCCGGCGCGTGGAGGACGCGGTG
AAAAAGGTGCTGGCCCAGGGCTACCGCACCGCCGACATCTACGAAGCCGGCTGCGAGAAGGTCAGTTGCTCCGGCATGGG
CGACGCGGTGGTGGCGGCGATGTGA

Upstream 100 bases:

>100_bases
GCCGCGATGGCGGCCTACATCGTGTTCAAATGTTGCGAAAAGGCGCTGGTGGATCGCTTGACCCGGCGCGATGAAGAGAA
ACGGAAATAAGGAAGAAACC

Downstream 100 bases:

>100_bases
CGCCGTTGGTCCGCTGATTGACGCGCGTGGCGCGATCCGCTGAAGGATCGCGCCATTTTTTATATAGATGGTCGGTTGTT
GATTGGCGTTTTAAATCCTT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKIAILPGDGIGPEIIAQAERVLEVLRRDGLKIETEHAPLGGAAYDQYGVPYPEATQKLAREADAVLLGAVGGPAYDKLD
RPLRPERGLLAIRKDLNLFANLRPAILYPELANASTLKPEVVAGLDIMIVRELTGDIYFGQPRGIAVNELGEREAYNTMR
YSESEVRRIAHVAFGIAMKRNRKLCSVDKANVLETTEFWKEIMIEVAKEYPQVELSHMYVDNAAMQLVRNPKQFDVMVTG
NIFGDILSDEASMLTGSIGMLPSASLDQNNKGLYEPSHGSAPDIAGQNLANPLATILSAAMMLRYSFGQEAAARRVEDAV
KKVLAQGYRTADIYEAGCEKVSCSGMGDAVVAAM

Sequences:

>Translated_354_residues
MKIAILPGDGIGPEIIAQAERVLEVLRRDGLKIETEHAPLGGAAYDQYGVPYPEATQKLAREADAVLLGAVGGPAYDKLD
RPLRPERGLLAIRKDLNLFANLRPAILYPELANASTLKPEVVAGLDIMIVRELTGDIYFGQPRGIAVNELGEREAYNTMR
YSESEVRRIAHVAFGIAMKRNRKLCSVDKANVLETTEFWKEIMIEVAKEYPQVELSHMYVDNAAMQLVRNPKQFDVMVTG
NIFGDILSDEASMLTGSIGMLPSASLDQNNKGLYEPSHGSAPDIAGQNLANPLATILSAAMMLRYSFGQEAAARRVEDAV
KKVLAQGYRTADIYEAGCEKVSCSGMGDAVVAAM
>Mature_354_residues
MKIAILPGDGIGPEIIAQAERVLEVLRRDGLKIETEHAPLGGAAYDQYGVPYPEATQKLAREADAVLLGAVGGPAYDKLD
RPLRPERGLLAIRKDLNLFANLRPAILYPELANASTLKPEVVAGLDIMIVRELTGDIYFGQPRGIAVNELGEREAYNTMR
YSESEVRRIAHVAFGIAMKRNRKLCSVDKANVLETTEFWKEIMIEVAKEYPQVELSHMYVDNAAMQLVRNPKQFDVMVTG
NIFGDILSDEASMLTGSIGMLPSASLDQNNKGLYEPSHGSAPDIAGQNLANPLATILSAAMMLRYSFGQEAAARRVEDAV
KKVLAQGYRTADIYEAGCEKVSCSGMGDAVVAAM

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=358, Percent_Identity=30.1675977653631, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI28178816, Length=357, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178821, Length=307, Percent_Identity=29.9674267100977, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI4758582, Length=306, Percent_Identity=29.7385620915033, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI28178838, Length=306, Percent_Identity=29.7385620915033, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=146, Percent_Identity=36.3013698630137, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=352, Percent_Identity=52.5568181818182, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=346, Percent_Identity=35.2601156069364, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=26.5508684863524, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=360, Percent_Identity=33.0555555555556, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=347, Percent_Identity=31.1239193083574, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=31.6568047337278, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=345, Percent_Identity=29.5652173913043, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=44.8753462603878, Blast_Score=282, Evalue=5e-77,
Organism=Saccharomyces cerevisiae, GI6322097, Length=346, Percent_Identity=36.4161849710983, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=32.1428571428571, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=31.9648093841642, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24643268, Length=359, Percent_Identity=31.4763231197772, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24643270, Length=359, Percent_Identity=31.4763231197772, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24661184, Length=364, Percent_Identity=29.6703296703297, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI161078633, Length=335, Percent_Identity=28.6567164179104, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24650122, Length=335, Percent_Identity=28.6567164179104, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078635, Length=335, Percent_Identity=28.6567164179104, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078637, Length=335, Percent_Identity=28.6567164179104, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078639, Length=333, Percent_Identity=28.8288288288288, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=28.2420749279539, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=28.2420749279539, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20130355, Length=338, Percent_Identity=24.2603550295858, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_CHRVO (Q7NUC2)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_902448.1
- ProteinModelPortal:   Q7NUC2
- SMR:   Q7NUC2
- GeneID:   2547144
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_2778
- NMPDR:   fig|243365.1.peg.2778
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   Q7NUC2
- ProtClustDB:   PRK00772
- BioCyc:   CVIO243365:CV_2778-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38545; Mature: 38545

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 93-93 BINDING 103-103 BINDING 131-131 BINDING 221-221

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAILPGDGIGPEIIAQAERVLEVLRRDGLKIETEHAPLGGAAYDQYGVPYPEATQKLA
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHH
READAVLLGAVGGPAYDKLDRPLRPERGLLAIRKDLNLFANLRPAILYPELANASTLKPE
HHCCEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
VVAGLDIMIVRELTGDIYFGQPRGIAVNELGEREAYNTMRYSESEVRRIAHVAFGIAMKR
HHHCCHHEEEEECCCCEEECCCCCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHC
NRKLCSVDKANVLETTEFWKEIMIEVAKEYPQVELSHMYVDNAAMQLVRNPKQFDVMVTG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEC
NIFGDILSDEASMLTGSIGMLPSASLDQNNKGLYEPSHGSAPDIAGQNLANPLATILSAA
HHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MMLRYSFGQEAAARRVEDAVKKVLAQGYRTADIYEAGCEKVSCSGMGDAVVAAM
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCHHHCC
>Mature Secondary Structure
MKIAILPGDGIGPEIIAQAERVLEVLRRDGLKIETEHAPLGGAAYDQYGVPYPEATQKLA
CEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHH
READAVLLGAVGGPAYDKLDRPLRPERGLLAIRKDLNLFANLRPAILYPELANASTLKPE
HHCCEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
VVAGLDIMIVRELTGDIYFGQPRGIAVNELGEREAYNTMRYSESEVRRIAHVAFGIAMKR
HHHCCHHEEEEECCCCEEECCCCCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHC
NRKLCSVDKANVLETTEFWKEIMIEVAKEYPQVELSHMYVDNAAMQLVRNPKQFDVMVTG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEC
NIFGDILSDEASMLTGSIGMLPSASLDQNNKGLYEPSHGSAPDIAGQNLANPLATILSAA
HHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
MMLRYSFGQEAAARRVEDAVKKVLAQGYRTADIYEAGCEKVSCSGMGDAVVAAM
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782