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The map label for this gene is metZ [H]

Identifier: 34498180

GI number: 34498180

Start: 2954870

End: 2956054

Strand: Reverse

Name: metZ [H]

Synonym: CV_2725

Alternate gene names: 34498180

Gene position: 2956054-2954870 (Counterclockwise)

Preceding gene: 34498181

Following gene: 34498179

Centisome position: 62.22

GC content: 66.5

Gene sequence:

>1185_bases
ATGGCATCCGACGCGCCGCATCTTCCGCTGCACCCTGAAACCCTGGCCATCCGGGCCGGGTTGGAAACCAGCCAGTTCAA
CGAGCACAGCCAGGGCCTGTTCCTGACGTCCAGCTTCACCTACGAATCGGCCGCGCAGGCGGCGGCGATGTTCCTGGGCG
AGATCGACGGCTACACCTATTCCCGCTTCACCAATCCGACCGTCGCCGCGTTCCAGCATAGGCTGGCGCAGATGGAGGGC
GGGGAGCGCGCCATCGCCACCGCCACCGGCATGGCGGCGATCCAGGCCATCATGATGACTTTGCTGCAGGCTGGCGACCA
CATCGTGTCGTCGCAAAGCCTGTTCGGCTCCACCACCAATCTGTTCGCCAACCAGTTGGCCAAGTTCGCCGTGGCCACCG
ACTTCGTCGACGCGCGCGACCTGTCCGCCTGGCGGGAGGCGCTGCGGCCGAACACCAAGCTGCTGTTCCTGGAGACGCCG
TCCAATCCCTTGACCGAAGTGGCCGACATCGCGGCCATCGCCGACATCGCCCACGCGCATGGCGCGCTGCTGGTGGTGGA
CAACAGCTTCTGTTCGCCGGCCTTGCAGCAGCCGTTGAAACTGGGCGCCGATCTGGTCATGCATTCCGCCACCAAGTTCA
TCGACGGCCATGGCCGGGTGATGGGCGGGGCGGTGGTCGGCAGCGACAAGCTGGTCGAGCAGGTCTATTTGCACGTGCGC
GCCGCCGGTCCCTCGCTGGCGCCGTTCAATGCCTGGACGCTGCTGTCCGGTTTGGAGACGCTGCACCTGCGGATGGAGAA
GCACAGCGCCAACGCGCTGGAGCTGGCGCGCTGGCTGGAGGCGCAGCCCAATGTGGAGCGCGTCTATTACCCGGGCCTGG
AGAGCCACCCCCAGCACGAGCTGGCGCTGCGCCAGCAGAAGAGCGGCGGAGCGGTGGTGTCCTTCGTGGTCAAGGGCGGC
CGCAAGGCCGCGTGGAAAGTGGTGGACGCGGTCAGGGTGATCTCGCGCACCGCCAATCTGGGCGATGTGAAAACCACCCT
CACTCATCCGGCCAGCACCACCCACGCCCGCGTGACGCAGGAGGCGCGCGAGCGCGCCGGCATCGTCGAGGGGCTGTTGC
GCGTCAGCGTCGGCCTGGAAAATGTACGGGACCTTCAACAAGATCTGTTGCGGGGCCTTGACTAA

Upstream 100 bases:

>100_bases
TGCATATGGCTTGACGGCATTTAGTAAACGCGGCGTTTTCAGGCAAGATGTCGGGATGCGGTCCGCCCGAGGGCGGACGA
GAAAGAAAGAGAGAATCATC

Downstream 100 bases:

>100_bases
GCCCTTTTAACGCGTGTTTCATTCAAACGGCAGTCTAAAATACGGACTGCCGTTTTTCGTGTGCCCTGAGCGGTGGATGG
CGAGGCAAGAGGGACATGCC

Product: O-succinylhomoserine sulfhydrylase

Products: cystathionine; succinate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MASDAPHLPLHPETLAIRAGLETSQFNEHSQGLFLTSSFTYESAAQAAAMFLGEIDGYTYSRFTNPTVAAFQHRLAQMEG
GERAIATATGMAAIQAIMMTLLQAGDHIVSSQSLFGSTTNLFANQLAKFAVATDFVDARDLSAWREALRPNTKLLFLETP
SNPLTEVADIAAIADIAHAHGALLVVDNSFCSPALQQPLKLGADLVMHSATKFIDGHGRVMGGAVVGSDKLVEQVYLHVR
AAGPSLAPFNAWTLLSGLETLHLRMEKHSANALELARWLEAQPNVERVYYPGLESHPQHELALRQQKSGGAVVSFVVKGG
RKAAWKVVDAVRVISRTANLGDVKTTLTHPASTTHARVTQEARERAGIVEGLLRVSVGLENVRDLQQDLLRGLD

Sequences:

>Translated_394_residues
MASDAPHLPLHPETLAIRAGLETSQFNEHSQGLFLTSSFTYESAAQAAAMFLGEIDGYTYSRFTNPTVAAFQHRLAQMEG
GERAIATATGMAAIQAIMMTLLQAGDHIVSSQSLFGSTTNLFANQLAKFAVATDFVDARDLSAWREALRPNTKLLFLETP
SNPLTEVADIAAIADIAHAHGALLVVDNSFCSPALQQPLKLGADLVMHSATKFIDGHGRVMGGAVVGSDKLVEQVYLHVR
AAGPSLAPFNAWTLLSGLETLHLRMEKHSANALELARWLEAQPNVERVYYPGLESHPQHELALRQQKSGGAVVSFVVKGG
RKAAWKVVDAVRVISRTANLGDVKTTLTHPASTTHARVTQEARERAGIVEGLLRVSVGLENVRDLQQDLLRGLD
>Mature_393_residues
ASDAPHLPLHPETLAIRAGLETSQFNEHSQGLFLTSSFTYESAAQAAAMFLGEIDGYTYSRFTNPTVAAFQHRLAQMEGG
ERAIATATGMAAIQAIMMTLLQAGDHIVSSQSLFGSTTNLFANQLAKFAVATDFVDARDLSAWREALRPNTKLLFLETPS
NPLTEVADIAAIADIAHAHGALLVVDNSFCSPALQQPLKLGADLVMHSATKFIDGHGRVMGGAVVGSDKLVEQVYLHVRA
AGPSLAPFNAWTLLSGLETLHLRMEKHSANALELARWLEAQPNVERVYYPGLESHPQHELALRQQKSGGAVVSFVVKGGR
KAAWKVVDAVRVISRTANLGDVKTTLTHPASTTHARVTQEARERAGIVEGLLRVSVGLENVRDLQQDLLRGLD

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=375, Percent_Identity=37.0666666666667, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI299473759, Length=376, Percent_Identity=34.0425531914894, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI25453487, Length=374, Percent_Identity=31.0160427807487, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1790375, Length=382, Percent_Identity=38.2198952879581, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789383, Length=340, Percent_Identity=27.0588235294118, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17560056, Length=375, Percent_Identity=37.0666666666667, Blast_Score=247, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI17538055, Length=376, Percent_Identity=37.2340425531915, Blast_Score=245, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71980708, Length=342, Percent_Identity=36.2573099415205, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=388, Percent_Identity=35.8247422680412, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=35.7798165137615, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321254, Length=406, Percent_Identity=28.5714285714286, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=284, Percent_Identity=29.9295774647887, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=343, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922554, Length=345, Percent_Identity=38.8405797101449, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48

Molecular weight: Translated: 42495; Mature: 42364

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDAPHLPLHPETLAIRAGLETSQFNEHSQGLFLTSSFTYESAAQAAAMFLGEIDGYTY
CCCCCCCCCCCCCCEEEECCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEE
SRFTNPTVAAFQHRLAQMEGGERAIATATGMAAIQAIMMTLLQAGDHIVSSQSLFGSTTN
ECCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCHHH
LFANQLAKFAVATDFVDARDLSAWREALRPNTKLLFLETPSNPLTEVADIAAIADIAHAH
HHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHC
GALLVVDNSFCSPALQQPLKLGADLVMHSATKFIDGHGRVMGGAVVGSDKLVEQVYLHVR
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEECHHHHHHHHHHHHE
AAGPSLAPFNAWTLLSGLETLHLRMEKHSANALELARWLEAQPNVERVYYPGLESHPQHE
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHH
LALRQQKSGGAVVSFVVKGGRKAAWKVVDAVRVISRTANLGDVKTTLTHPASTTHARVTQ
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCHHHHHHH
EARERAGIVEGLLRVSVGLENVRDLQQDLLRGLD
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASDAPHLPLHPETLAIRAGLETSQFNEHSQGLFLTSSFTYESAAQAAAMFLGEIDGYTY
CCCCCCCCCCCCCEEEECCCCHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEE
SRFTNPTVAAFQHRLAQMEGGERAIATATGMAAIQAIMMTLLQAGDHIVSSQSLFGSTTN
ECCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCHHH
LFANQLAKFAVATDFVDARDLSAWREALRPNTKLLFLETPSNPLTEVADIAAIADIAHAH
HHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHC
GALLVVDNSFCSPALQQPLKLGADLVMHSATKFIDGHGRVMGGAVVGSDKLVEQVYLHVR
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEECHHHHHHHHHHHHE
AAGPSLAPFNAWTLLSGLETLHLRMEKHSANALELARWLEAQPNVERVYYPGLESHPQHE
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHH
LALRQQKSGGAVVSFVVKGGRKAAWKVVDAVRVISRTANLGDVKTTLTHPASTTHARVTQ
HHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCHHHHHHH
EARERAGIVEGLLRVSVGLENVRDLQQDLLRGLD
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; L-cysteine

Specific reaction: O-succinyl-L-homoserine + L-cysteine = cystathionine + succinate

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]