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The map label for this gene is phoB [H]

Identifier: 34498099

GI number: 34498099

Start: 2857720

End: 2859420

Strand: Reverse

Name: phoB [H]

Synonym: CV_2644

Alternate gene names: 34498099

Gene position: 2859420-2857720 (Counterclockwise)

Preceding gene: 34498100

Following gene: 34498098

Centisome position: 60.18

GC content: 64.2

Gene sequence:

>1701_bases
ATGGATCGCACTTTCACTGTGCTGGTGGCCGAGGATGTCGAGCCGACCCGTCTGCTGATGGGGCTGGTGATCGAGGAGAT
GGGCCATCAGGTCTGGTACGCCGAAAATGGCCAGCAGGCCGTCGAGCAATGCAGGCGCGAGCTGCCCGACATGGTGTTGA
TGGACATGATGATGCCGGTGATGGACGGCCACGAGGCCACCCGGCAGATCCGCCAACTGTGCGGCGACCGCTGGCTGCCC
ATCATCTTCCTCAGCGCCCGCAGCGACGAAGAGGCGCATATCAGCAGCCTGGACGTGGGCGCCGACGATTACCTGCCCAA
GCCGATCAATCTGACCATGCTGGCGGCCAAGATCCGCGTGATGCAGCGCATCAGCGAAATGCAGCGGCGGATTTCCGACG
ATTCCCAGCAGTTGGTCAGCTACTACCACAGCAACGAGCGCGAGCAGCTGTTCGCCAAGCATGTGCTGGACAGCATCGTC
GGCAGCGAGGAGCAGGATCTGCCCGGCGTCTACAGCTGGCAGCAGTCCGCGGAGCAGTTCAGCGGCGACGTGCTGGCGCG
CGCGCTGTCGCCGACCGGGCAGCTGTGCGTGATGCTGGCGGACAGCACCGGGCACGGTTTGTCCGCGGCCTTGTCCTGCC
TGCCGGCGGTGGACATCTTCTACGCGATGGCGAGGCGCGGCTTCCCTTTGACGTCAATGGCTAGGGAGATCAACGCCAAG
CTCAACCGGCTGCTGCCGGTGGGGCGATTCGTCGCCGCGGCGCTGATCACCATCGACTGGGAGTTGGGTTGCGTCGAGGT
TTGGAACGGCGGCATCCCCTGCGTGATGTTCGTCGACGACGAAGGTTGTCCGATGAAGGAATGGAAGTCGCAATATCCGC
CATTGGGCATCTTGTCTCCCGACGAGTTTTGCTGGGGCACCGAAACCTATTTCTGGGAGCAGAGCGGACAGCTGGTGGTG
TGCTCCGACGGCTTGACCGAGGCCGAGAACCGGGCGGGCGAACAGTTCGGCATCGGAGGGTTGCTGCAGGCGTTGATTGC
GGGCCCGTCCTCGCCGCTCAACAACGTTCGGTCGGCAGTGCTCGGGCACCTGGGCGGCGCCAACAACCGCGACGACATCT
CGCTGGGCGTGGTCAGGCTGGAGGCCGGCGTCAACGCCGCGCTGGACCGGGTGGCCGACCACCTGAGTCGCGACGACGGC
GGGCAGTTGAGCACCTGGAAAGTCACCCTGGCCTTCAGCGCGGAGGAACTGAAGCGCGATCAGAGCATTACGCTGCTGAT
CGGCTGGCTGAATCAGCTCGGTTTCCGCGGCCGGCAGTTCAAGGAAGCGCTGCTGATCGTATCCGAGCTGTTCAACAACG
CCCTGGACCACGGGGTGCTGGGGCTGGATTCGACGATGAAGGATCAGGCGGACGGCTACGAACGCTATTTCATGTTGAGG
CGGGAACGGCTGGGGGCGCTGGAGAGGGCTGAAATCGAGCTGACGCTTGAGCGCCGGCGCGGCGAAAATGACGGCGATGC
CGTGCGGATACGCATCCGGGACAGCGGCGGCGGGTTCGATCATCCGACGCTGATGGAAAAACTGGGCCGGCAGGCTGGCG
GCCATCGGCACGGGCGCGGCGTATCTCTGGTGCATGCCTTGTGCTTGAGCTTGGTTTACCACGGCAGCGGCAACGAAGTG
GAGGCGGAATTCAGGCTGTAG

Upstream 100 bases:

>100_bases
GGCATAGAAGGCATCGCGCTGACAGGGTGCCGCGGCTTCGTGCGCAAGATTCTCGACGTGGCCAGCTTCGACAAGTTCTT
CACCATCCACTGAGCATCCC

Downstream 100 bases:

>100_bases
GGTTTCGCCATCGACAACAGGGAGCCTGGTCTGATGATGTCCTTGTCCGACAGCCTGTCCACGCCGTCCCGGCGCGCCGC
CGCCGGGCTGGCCGGCGCCG

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MDRTFTVLVAEDVEPTRLLMGLVIEEMGHQVWYAENGQQAVEQCRRELPDMVLMDMMMPVMDGHEATRQIRQLCGDRWLP
IIFLSARSDEEAHISSLDVGADDYLPKPINLTMLAAKIRVMQRISEMQRRISDDSQQLVSYYHSNEREQLFAKHVLDSIV
GSEEQDLPGVYSWQQSAEQFSGDVLARALSPTGQLCVMLADSTGHGLSAALSCLPAVDIFYAMARRGFPLTSMAREINAK
LNRLLPVGRFVAAALITIDWELGCVEVWNGGIPCVMFVDDEGCPMKEWKSQYPPLGILSPDEFCWGTETYFWEQSGQLVV
CSDGLTEAENRAGEQFGIGGLLQALIAGPSSPLNNVRSAVLGHLGGANNRDDISLGVVRLEAGVNAALDRVADHLSRDDG
GQLSTWKVTLAFSAEELKRDQSITLLIGWLNQLGFRGRQFKEALLIVSELFNNALDHGVLGLDSTMKDQADGYERYFMLR
RERLGALERAEIELTLERRRGENDGDAVRIRIRDSGGGFDHPTLMEKLGRQAGGHRHGRGVSLVHALCLSLVYHGSGNEV
EAEFRL

Sequences:

>Translated_566_residues
MDRTFTVLVAEDVEPTRLLMGLVIEEMGHQVWYAENGQQAVEQCRRELPDMVLMDMMMPVMDGHEATRQIRQLCGDRWLP
IIFLSARSDEEAHISSLDVGADDYLPKPINLTMLAAKIRVMQRISEMQRRISDDSQQLVSYYHSNEREQLFAKHVLDSIV
GSEEQDLPGVYSWQQSAEQFSGDVLARALSPTGQLCVMLADSTGHGLSAALSCLPAVDIFYAMARRGFPLTSMAREINAK
LNRLLPVGRFVAAALITIDWELGCVEVWNGGIPCVMFVDDEGCPMKEWKSQYPPLGILSPDEFCWGTETYFWEQSGQLVV
CSDGLTEAENRAGEQFGIGGLLQALIAGPSSPLNNVRSAVLGHLGGANNRDDISLGVVRLEAGVNAALDRVADHLSRDDG
GQLSTWKVTLAFSAEELKRDQSITLLIGWLNQLGFRGRQFKEALLIVSELFNNALDHGVLGLDSTMKDQADGYERYFMLR
RERLGALERAEIELTLERRRGENDGDAVRIRIRDSGGGFDHPTLMEKLGRQAGGHRHGRGVSLVHALCLSLVYHGSGNEV
EAEFRL
>Mature_566_residues
MDRTFTVLVAEDVEPTRLLMGLVIEEMGHQVWYAENGQQAVEQCRRELPDMVLMDMMMPVMDGHEATRQIRQLCGDRWLP
IIFLSARSDEEAHISSLDVGADDYLPKPINLTMLAAKIRVMQRISEMQRRISDDSQQLVSYYHSNEREQLFAKHVLDSIV
GSEEQDLPGVYSWQQSAEQFSGDVLARALSPTGQLCVMLADSTGHGLSAALSCLPAVDIFYAMARRGFPLTSMAREINAK
LNRLLPVGRFVAAALITIDWELGCVEVWNGGIPCVMFVDDEGCPMKEWKSQYPPLGILSPDEFCWGTETYFWEQSGQLVV
CSDGLTEAENRAGEQFGIGGLLQALIAGPSSPLNNVRSAVLGHLGGANNRDDISLGVVRLEAGVNAALDRVADHLSRDDG
GQLSTWKVTLAFSAEELKRDQSITLLIGWLNQLGFRGRQFKEALLIVSELFNNALDHGVLGLDSTMKDQADGYERYFMLR
RERLGALERAEIELTLERRRGENDGDAVRIRIRDSGGGFDHPTLMEKLGRQAGGHRHGRGVSLVHALCLSLVYHGSGNEV
EAEFRL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=37.7049180327869, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=38.135593220339, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=31.3559322033898, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787375, Length=134, Percent_Identity=33.5820895522388, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786784, Length=139, Percent_Identity=30.2158273381295, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=35.2380952380952, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=32.4786324786325, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=33.0645161290323, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1788394, Length=134, Percent_Identity=29.1044776119403, Blast_Score=63, Evalue=6e-11,
Organism=Escherichia coli, GI1788550, Length=137, Percent_Identity=27.7372262773723, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 62848; Mature: 62848

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRTFTVLVAEDVEPTRLLMGLVIEEMGHQVWYAENGQQAVEQCRRELPDMVLMDMMMPV
CCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCHHHHHHHHHHHH
MDGHEATRQIRQLCGDRWLPIIFLSARSDEEAHISSLDVGADDYLPKPINLTMLAAKIRV
HCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
MQRISEMQRRISDDSQQLVSYYHSNEREQLFAKHVLDSIVGSEEQDLPGVYSWQQSAEQF
HHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SGDVLARALSPTGQLCVMLADSTGHGLSAALSCLPAVDIFYAMARRGFPLTSMAREINAK
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LNRLLPVGRFVAAALITIDWELGCVEVWNGGIPCVMFVDDEGCPMKEWKSQYPPLGILSP
HHHHCHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEECCCCCCHHHHHHCCCCCCCCCC
DEFCWGTETYFWEQSGQLVVCSDGLTEAENRAGEQFGIGGLLQALIAGPSSPLNNVRSAV
CCCCCCCCCEEEECCCCEEEECCCCCHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
LGHLGGANNRDDISLGVVRLEAGVNAALDRVADHLSRDDGGQLSTWKVTLAFSAEELKRD
HHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECHHHHCCC
QSITLLIGWLNQLGFRGRQFKEALLIVSELFNNALDHGVLGLDSTMKDQADGYERYFMLR
CCEEHHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHH
RERLGALERAEIELTLERRRGENDGDAVRIRIRDSGGGFDHPTLMEKLGRQAGGHRHGRG
HHHHCCCHHHHEEEEEHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCC
VSLVHALCLSLVYHGSGNEVEAEFRL
HHHHHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MDRTFTVLVAEDVEPTRLLMGLVIEEMGHQVWYAENGQQAVEQCRRELPDMVLMDMMMPV
CCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCHHHHHHHHHHHH
MDGHEATRQIRQLCGDRWLPIIFLSARSDEEAHISSLDVGADDYLPKPINLTMLAAKIRV
HCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
MQRISEMQRRISDDSQQLVSYYHSNEREQLFAKHVLDSIVGSEEQDLPGVYSWQQSAEQF
HHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
SGDVLARALSPTGQLCVMLADSTGHGLSAALSCLPAVDIFYAMARRGFPLTSMAREINAK
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LNRLLPVGRFVAAALITIDWELGCVEVWNGGIPCVMFVDDEGCPMKEWKSQYPPLGILSP
HHHHCHHHHHHHHHHHEEECCCCEEEEECCCCCEEEEECCCCCCHHHHHHCCCCCCCCCC
DEFCWGTETYFWEQSGQLVVCSDGLTEAENRAGEQFGIGGLLQALIAGPSSPLNNVRSAV
CCCCCCCCCEEEECCCCEEEECCCCCHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHH
LGHLGGANNRDDISLGVVRLEAGVNAALDRVADHLSRDDGGQLSTWKVTLAFSAEELKRD
HHHCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECHHHHCCC
QSITLLIGWLNQLGFRGRQFKEALLIVSELFNNALDHGVLGLDSTMKDQADGYERYFMLR
CCEEHHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCHHHHHHHHH
RERLGALERAEIELTLERRRGENDGDAVRIRIRDSGGGFDHPTLMEKLGRQAGGHRHGRG
HHHHCCCHHHHEEEEEHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCC
VSLVHALCLSLVYHGSGNEVEAEFRL
HHHHHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]