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The map label for this gene is sipA [H]

Identifier: 34498071

GI number: 34498071

Start: 2822273

End: 2824330

Strand: Reverse

Name: sipA [H]

Synonym: CV_2616

Alternate gene names: 34498071

Gene position: 2824330-2822273 (Counterclockwise)

Preceding gene: 34498072

Following gene: 34498070

Centisome position: 59.45

GC content: 71.72

Gene sequence:

>2058_bases
ATGCCCATCACAGGAGCTTCCCACGCCGCGATGCGCCCCTTGTCCGCGCCGCCGGACGGAACGGCCGGCCCCGCCAGTCC
GCTGGGACGGCAATTGCGCGATGTGCGCGCCGACAGCGTCGAACCCGCGCGCCGCCATTCGCTGGCCGATCTGGCCAGCT
ACCAGGCCGACTACGCCCGCCGCAGCGTGGCGACGCTGTTCGCCGCCTCGCCGCATGCCGATAAGCTGGGCGAGCTGTAC
CAGGCCTCGCCCAATCGCTACGCCAAGCTGGAGATCGCCGAATTCGCCAAGGTCTACAGCCAGCTCAAGCGGCAGCCGGA
TCTCGACCCCGCCGCAGGCAAGACGCTGGACGACCTGGCGCAGCAGTACGCCGCCCGAATCCTGAAGGATGGCTTGGGGG
AGAAGTCCGCTTTCGGCCCCTGGACCCAGAGAACCGACAAGCACTATCAGCTGCGCAGCGGCCTGGAGCGCAAGCTGGCG
GAGATCGCCAGCCGGCACTGCCAGGGCGATGCGCAAAAGCTGGGCAACGATTTCATGCGCGCGGAGGTGACCACCTTCAT
CCTGTCCTGCGTAGAAACCCATCTGGGGCGACAGCTGGACGAGGCCACCAGCCGGCAGATCACCGGGCTGGTGGACAGCG
CGGCGATGCAGGCCTTCGAAGATCTGCGCCAGCGCCGCGGCGACCTGATCGAGCAGCGCGGTTTCAGCGTGGGCACGCTG
GCGCGCGACCTCGATACCGTGGCGGTGCTGCCGCAATTGCTGCGCAGCCTGCTGGAGGCCTTGCCGCCCGGTCCGGGCCA
ACGGGCGCCGGAGGAGCCTGCGCGCGACGGTCCGGCCAGGCCGACGCCCAGCCCGGACCCCGGCCCGGCGGGGCCGGGAG
AGGCGCAGCGGCCGCAGGAAATCCATTACCACATCGACAACAGCATCCACTGGAACGACAACAGCCAGGACAACCGGCGC
TGGAACCGGCGCGGCGACACCTACCTGGGCGGCGCGCGGCAGGGCGACCGCCATCTCCGTTCGTCCGGTTTCCCCGCGTC
GGGGCTGGCGGCGTCGCTGCGGACCGCGGCCAGCCAGGATCTGCCCAAGCCGCAGCAGGCGGTGTTGTCCGGGCCGGCCT
CGCCTGCCGCTGGGCGCCATCCGCTGCTGAATGCCGTCGACCAGGTGGGACAGAGCCTGTCCGGGCTGGTGGACGCGGCC
ATCGGAACCGCCGGCACCCGGCCATTGTCGCCGGGCGGGGCAGCCGAATCATCGGCTGTCAGGCTGGCGGGCCTCGGGAG
CGACGGCCTGAGCGCGCTGCCGCCGCGGGACGATCCGGGCGCAAGCCTGCATGGCGCGGCCCGCGCCGTGGCAGACAGCC
TGTCCGCCGTGCTGACGGCGGCGGACGGCGCGAAAATCACGAAAATCGCGACGCCGGCGCGGCTGGATGCCGCCGCGAGC
GGCGAGGACGCGCAGCAGCCTGCTGCCGGCGAGGCGATCCCGGCCGACGCCGGCCAGCCGGGCGCAGGCGGCTTCGACGG
CATCAGGTTCCGCGATGGCAATCTGTACATGCTGCCGACCCAGGCCTATCTGCGCGGCCTCGCGTCTCCGCGCAGGGCCG
AGAACGAGCTGTTGCGGGCGGTGCGCGGCGCGCTGGAGCCGGCCGCCGCTCAGCCGATGGCGCAGCGGCGGGAATTCGAG
GCGTTGCGCAACCGTATCCTGCCCAGCGACCGTTTCGACCAGGATAAGGTGCTGGACCGTTTCGCTTCCGGCGGCGAAGA
CCGCTTGGCCGACGACGCGGCGCGGCTGCGGCAAGCGCTGGCCGGGCATCCCGGACTGGAGCGGCATCGCCAGGCCCTGC
GCAGTTTCGCGCGCACCCTGATACGCGAGGCCAACCTGTTGCCCAGCGCCAAGCCGGCCAATCCGCTGGTGGCCGGGCTG
CTGGACGCGCTGGGCATCCAGGCGGACGAGGAGGCGCGCCGCGCGCCGCTGCCGAGCAAACCATGCGGCGTGGTTCTGAC
CACGGATGGCCTGCATGTCGATTCCAGTCGCGCCGGAGGCCGTCGCGGTGCATCGTGA

Upstream 100 bases:

>100_bases
CTGTTCGACAACCTGGTCAAGGTGCTCAGCAGCACCATCAGCAGCTGCCTGGAAACCGCCAAGTCCTTCCTGCAAATCTG
AACGCACAGGAGCGCCACAC

Downstream 100 bases:

>100_bases
TATCGAATCCATGGTCAGGGAGGTGATGGCGTACAGCCTTTCCGCCGATGCCGGGCGCTTGCAGCCTCAGGCCAGGCTGG
TGGACGATTTGTACGCCGAC

Product: invasion protein

Products: NA

Alternate protein names: 70 kDa antigen [H]

Number of amino acids: Translated: 685; Mature: 684

Protein sequence:

>685_residues
MPITGASHAAMRPLSAPPDGTAGPASPLGRQLRDVRADSVEPARRHSLADLASYQADYARRSVATLFAASPHADKLGELY
QASPNRYAKLEIAEFAKVYSQLKRQPDLDPAAGKTLDDLAQQYAARILKDGLGEKSAFGPWTQRTDKHYQLRSGLERKLA
EIASRHCQGDAQKLGNDFMRAEVTTFILSCVETHLGRQLDEATSRQITGLVDSAAMQAFEDLRQRRGDLIEQRGFSVGTL
ARDLDTVAVLPQLLRSLLEALPPGPGQRAPEEPARDGPARPTPSPDPGPAGPGEAQRPQEIHYHIDNSIHWNDNSQDNRR
WNRRGDTYLGGARQGDRHLRSSGFPASGLAASLRTAASQDLPKPQQAVLSGPASPAAGRHPLLNAVDQVGQSLSGLVDAA
IGTAGTRPLSPGGAAESSAVRLAGLGSDGLSALPPRDDPGASLHGAARAVADSLSAVLTAADGAKITKIATPARLDAAAS
GEDAQQPAAGEAIPADAGQPGAGGFDGIRFRDGNLYMLPTQAYLRGLASPRRAENELLRAVRGALEPAAAQPMAQRREFE
ALRNRILPSDRFDQDKVLDRFASGGEDRLADDAARLRQALAGHPGLERHRQALRSFARTLIREANLLPSAKPANPLVAGL
LDALGIQADEEARRAPLPSKPCGVVLTTDGLHVDSSRAGGRRGAS

Sequences:

>Translated_685_residues
MPITGASHAAMRPLSAPPDGTAGPASPLGRQLRDVRADSVEPARRHSLADLASYQADYARRSVATLFAASPHADKLGELY
QASPNRYAKLEIAEFAKVYSQLKRQPDLDPAAGKTLDDLAQQYAARILKDGLGEKSAFGPWTQRTDKHYQLRSGLERKLA
EIASRHCQGDAQKLGNDFMRAEVTTFILSCVETHLGRQLDEATSRQITGLVDSAAMQAFEDLRQRRGDLIEQRGFSVGTL
ARDLDTVAVLPQLLRSLLEALPPGPGQRAPEEPARDGPARPTPSPDPGPAGPGEAQRPQEIHYHIDNSIHWNDNSQDNRR
WNRRGDTYLGGARQGDRHLRSSGFPASGLAASLRTAASQDLPKPQQAVLSGPASPAAGRHPLLNAVDQVGQSLSGLVDAA
IGTAGTRPLSPGGAAESSAVRLAGLGSDGLSALPPRDDPGASLHGAARAVADSLSAVLTAADGAKITKIATPARLDAAAS
GEDAQQPAAGEAIPADAGQPGAGGFDGIRFRDGNLYMLPTQAYLRGLASPRRAENELLRAVRGALEPAAAQPMAQRREFE
ALRNRILPSDRFDQDKVLDRFASGGEDRLADDAARLRQALAGHPGLERHRQALRSFARTLIREANLLPSAKPANPLVAGL
LDALGIQADEEARRAPLPSKPCGVVLTTDGLHVDSSRAGGRRGAS
>Mature_684_residues
PITGASHAAMRPLSAPPDGTAGPASPLGRQLRDVRADSVEPARRHSLADLASYQADYARRSVATLFAASPHADKLGELYQ
ASPNRYAKLEIAEFAKVYSQLKRQPDLDPAAGKTLDDLAQQYAARILKDGLGEKSAFGPWTQRTDKHYQLRSGLERKLAE
IASRHCQGDAQKLGNDFMRAEVTTFILSCVETHLGRQLDEATSRQITGLVDSAAMQAFEDLRQRRGDLIEQRGFSVGTLA
RDLDTVAVLPQLLRSLLEALPPGPGQRAPEEPARDGPARPTPSPDPGPAGPGEAQRPQEIHYHIDNSIHWNDNSQDNRRW
NRRGDTYLGGARQGDRHLRSSGFPASGLAASLRTAASQDLPKPQQAVLSGPASPAAGRHPLLNAVDQVGQSLSGLVDAAI
GTAGTRPLSPGGAAESSAVRLAGLGSDGLSALPPRDDPGASLHGAARAVADSLSAVLTAADGAKITKIATPARLDAAASG
EDAQQPAAGEAIPADAGQPGAGGFDGIRFRDGNLYMLPTQAYLRGLASPRRAENELLRAVRGALEPAAAQPMAQRREFEA
LRNRILPSDRFDQDKVLDRFASGGEDRLADDAARLRQALAGHPGLERHRQALRSFARTLIREANLLPSAKPANPLVAGLL
DALGIQADEEARRAPLPSKPCGVVLTTDGLHVDSSRAGGRRGAS

Specific function: Rapidly associates with the first 265 amino acids of vinculin after bacteria-cell contact. This interaction is critical for efficient Shigella uptake. IpaA acts as a potent activator of vinculin and increase its ability to interact with F-actin. The compl

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted through the specialized type-III secretion system Mxi/Spa from the bacterium through the cell cytosol [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sipA/ipaA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015138
- InterPro:   IPR023225 [H]

Pfam domain/function: PF09052 SipA [H]

EC number: NA

Molecular weight: Translated: 72580; Mature: 72449

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITGASHAAMRPLSAPPDGTAGPASPLGRQLRDVRADSVEPARRHSLADLASYQADYAR
CCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
RSVATLFAASPHADKLGELYQASPNRYAKLEIAEFAKVYSQLKRQPDLDPAAGKTLDDLA
HHHHHHHHCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
QQYAARILKDGLGEKSAFGPWTQRTDKHYQLRSGLERKLAEIASRHCQGDAQKLGNDFMR
HHHHHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AEVTTFILSCVETHLGRQLDEATSRQITGLVDSAAMQAFEDLRQRRGDLIEQRGFSVGTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ARDLDTVAVLPQLLRSLLEALPPGPGQRAPEEPARDGPARPTPSPDPGPAGPGEAQRPQE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
IHYHIDNSIHWNDNSQDNRRWNRRGDTYLGGARQGDRHLRSSGFPASGLAASLRTAASQD
HHEEECCEEECCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHC
LPKPQQAVLSGPASPAAGRHPLLNAVDQVGQSLSGLVDAAIGTAGTRPLSPGGAAESSAV
CCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCE
RLAGLGSDGLSALPPRDDPGASLHGAARAVADSLSAVLTAADGAKITKIATPARLDAAAS
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCC
GEDAQQPAAGEAIPADAGQPGAGGFDGIRFRDGNLYMLPTQAYLRGLASPRRAENELLRA
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEECHHHHHHHHCCCHHHHHHHHHH
VRGALEPAAAQPMAQRREFEALRNRILPSDRFDQDKVLDRFASGGEDRLADDAARLRQAL
HHHHCCHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AGHPGLERHRQALRSFARTLIREANLLPSAKPANPLVAGLLDALGIQADEEARRAPLPSK
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCC
PCGVVLTTDGLHVDSSRAGGRRGAS
CCCEEEECCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
PITGASHAAMRPLSAPPDGTAGPASPLGRQLRDVRADSVEPARRHSLADLASYQADYAR
CCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH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CCCEEEECCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183200; 11115111; 11292750; 12384590; 3057506; 9184218; 10545097 [H]