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The map label for this gene is narK2 [H]

Identifier: 34497999

GI number: 34497999

Start: 2736928

End: 2738307

Strand: Reverse

Name: narK2 [H]

Synonym: CV_2544

Alternate gene names: 34497999

Gene position: 2738307-2736928 (Counterclockwise)

Preceding gene: 34498000

Following gene: 34497998

Centisome position: 57.64

GC content: 65.22

Gene sequence:

>1380_bases
ATGACTTATCTGATCAAACACTGGGAGCCGGAATCGGCTTCGTTCTGGCAGCAGCAGGGACGCTCGGTGGCGCGGCGCAA
CCTGTGGATCTCGATTCCGGCGCTGACGCTGGCCTTCGTGATCTGGCAGGTGTGGAGCGTGGCGGTGCTGAACATGCCCA
ATATCGGCTTCGGCTACACCCAGAACCAGCTGTTCTGGCTGGCGGCGCTGCCGGCCTTGTCCGGCGCCACGCTGCGCATC
TTCTATTCCTTCATGGTGCCGGTGTTCGGCGGCCGCAAGTGGACGGCGATCTCCACCGCCAGCCTGCTGATTCCGGCGGT
GGGCATAGGCTTCGCGGTGCAGAACCCGGACACCAGCTATGTGACCATGGTGACGCTGGCGCTGCTGTGCGGCTTCGGCG
GCGGCAATTTCAGCTCCAGCATGGCCAATATCAGCTTCTTCTTCCCCAAGTCGGAAAAGGGCACCGCGCTGGGCCTGAAC
GCCGGCCTGGGCAATCTGGGCGTATCCATCGTGCAGTTCGTGGTGCCCATCGTCATCACCATGGGCCTGTTCGGCCAGTT
GGGCGGCGATCCGCAGCACTGGGTGAAGGGCGCGGCAGCCAAACAGATGTGGCTGCAGAACGCCGGCTTCGTCTGGGTGC
CGTTCATCGTGCTGTCCACGCTGGCGGCCTGGTTCGGCATGAACGACCTGGCCTCGGCCAAATCCTCGTTTTCCGAGCAG
GCGGTGATCTTCACCCGCAAGCATAACTGGCTGATGTGCTGGCTGTACGTGGGCACCTTCGGTTCTTTCATCGGCTTCTC
CGCCGGCTTCCCGCTGCTGGTCAAGGGCCAGTTCCCCGACATCGACCCCACCCGCTACGCCTTCCTGGGCCCCTTGGTCG
GCGCGCTGACCCGCCCGCTGGGCGGCTGGCTGGCCGACAAGATAGGCGGCGCCAAGGTGACGCAGACGGTGTTCGCGCTG
ATGGTGCTGGGCGTGCTGGGCGTGATCTTTTTCCTGCCGCACGGCGGCCAGGGCGGCAACTTCTACGGTTTCTTCGCGAT
GTTCCTGTGCCTGTTCGCGCTGACCGGCGTCGGCAATGGCTCCACCTTCATGCAGGTGCCGGCCATTTTCCTGACGCTGC
ACCAGCGCATGGCCGGCCCGGGCGAGTCGGCCATGCGGCAGGCCACCCAGGACGCCACCCGCGAGGCGGCGGCGGTGCTC
GGTTTCGCCGGCGCGATCGGCGCCTACGGCGGCTTCTTCATCCCCAAGAGCTACGGCACCTCCATCGCGATGACCGGCGG
GGTAGAGGCGGCGCTGTACCTGTTCATCGCCTTCTACCTGAGCTGCCTGGCGATCAACGGCTGGTACTACGCCCGCAAGA
ACGCGGAAGTGCGTTGCTGA

Upstream 100 bases:

>100_bases
AATCGGCGGCAAGAGCCAAGGCGCGGGCGCGCGCGGCCGAGCCCGTCAATCCCATTCCCTTGACCAACGAACGCGGCTGA
GCGCGGCGCGGGAGACACTG

Downstream 100 bases:

>100_bases
TTGGCCGGCGCAATGCCCGGCAGAGCGTGCTAGCCGGGTATTGCACCCTACGGTTGGACGGAATGAAAAGCGCCCGCGGG
CGCTTTTTTCCCGTCCGGCT

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTYLIKHWEPESASFWQQQGRSVARRNLWISIPALTLAFVIWQVWSVAVLNMPNIGFGYTQNQLFWLAALPALSGATLRI
FYSFMVPVFGGRKWTAISTASLLIPAVGIGFAVQNPDTSYVTMVTLALLCGFGGGNFSSSMANISFFFPKSEKGTALGLN
AGLGNLGVSIVQFVVPIVITMGLFGQLGGDPQHWVKGAAAKQMWLQNAGFVWVPFIVLSTLAAWFGMNDLASAKSSFSEQ
AVIFTRKHNWLMCWLYVGTFGSFIGFSAGFPLLVKGQFPDIDPTRYAFLGPLVGALTRPLGGWLADKIGGAKVTQTVFAL
MVLGVLGVIFFLPHGGQGGNFYGFFAMFLCLFALTGVGNGSTFMQVPAIFLTLHQRMAGPGESAMRQATQDATREAAAVL
GFAGAIGAYGGFFIPKSYGTSIAMTGGVEAALYLFIAFYLSCLAINGWYYARKNAEVRC

Sequences:

>Translated_459_residues
MTYLIKHWEPESASFWQQQGRSVARRNLWISIPALTLAFVIWQVWSVAVLNMPNIGFGYTQNQLFWLAALPALSGATLRI
FYSFMVPVFGGRKWTAISTASLLIPAVGIGFAVQNPDTSYVTMVTLALLCGFGGGNFSSSMANISFFFPKSEKGTALGLN
AGLGNLGVSIVQFVVPIVITMGLFGQLGGDPQHWVKGAAAKQMWLQNAGFVWVPFIVLSTLAAWFGMNDLASAKSSFSEQ
AVIFTRKHNWLMCWLYVGTFGSFIGFSAGFPLLVKGQFPDIDPTRYAFLGPLVGALTRPLGGWLADKIGGAKVTQTVFAL
MVLGVLGVIFFLPHGGQGGNFYGFFAMFLCLFALTGVGNGSTFMQVPAIFLTLHQRMAGPGESAMRQATQDATREAAAVL
GFAGAIGAYGGFFIPKSYGTSIAMTGGVEAALYLFIAFYLSCLAINGWYYARKNAEVRC
>Mature_458_residues
TYLIKHWEPESASFWQQQGRSVARRNLWISIPALTLAFVIWQVWSVAVLNMPNIGFGYTQNQLFWLAALPALSGATLRIF
YSFMVPVFGGRKWTAISTASLLIPAVGIGFAVQNPDTSYVTMVTLALLCGFGGGNFSSSMANISFFFPKSEKGTALGLNA
GLGNLGVSIVQFVVPIVITMGLFGQLGGDPQHWVKGAAAKQMWLQNAGFVWVPFIVLSTLAAWFGMNDLASAKSSFSEQA
VIFTRKHNWLMCWLYVGTFGSFIGFSAGFPLLVKGQFPDIDPTRYAFLGPLVGALTRPLGGWLADKIGGAKVTQTVFALM
VLGVLGVIFFLPHGGQGGNFYGFFAMFLCLFALTGVGNGSTFMQVPAIFLTLHQRMAGPGESAMRQATQDATREAAAVLG
FAGAIGAYGGFFIPKSYGTSIAMTGGVEAALYLFIAFYLSCLAINGWYYARKNAEVRC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=458, Percent_Identity=51.528384279476, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=49.1266375545852, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49490; Mature: 49359

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYLIKHWEPESASFWQQQGRSVARRNLWISIPALTLAFVIWQVWSVAVLNMPNIGFGYT
CCEEEECCCCCCHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QNQLFWLAALPALSGATLRIFYSFMVPVFGGRKWTAISTASLLIPAVGIGFAVQNPDTSY
CCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCEEECCCCCHH
VTMVTLALLCGFGGGNFSSSMANISFFFPKSEKGTALGLNAGLGNLGVSIVQFVVPIVIT
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
MGLFGQLGGDPQHWVKGAAAKQMWLQNAGFVWVPFIVLSTLAAWFGMNDLASAKSSFSEQ
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
AVIFTRKHNWLMCWLYVGTFGSFIGFSAGFPLLVKGQFPDIDPTRYAFLGPLVGALTRPL
EEEEEECCCEEEEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
GGWLADKIGGAKVTQTVFALMVLGVLGVIFFLPHGGQGGNFYGFFAMFLCLFALTGVGNG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
STFMQVPAIFLTLHQRMAGPGESAMRQATQDATREAAAVLGFAGAIGAYGGFFIPKSYGT
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
SIAMTGGVEAALYLFIAFYLSCLAINGWYYARKNAEVRC
EEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
>Mature Secondary Structure 
TYLIKHWEPESASFWQQQGRSVARRNLWISIPALTLAFVIWQVWSVAVLNMPNIGFGYT
CEEEECCCCCCHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QNQLFWLAALPALSGATLRIFYSFMVPVFGGRKWTAISTASLLIPAVGIGFAVQNPDTSY
CCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCEEECCCCCHH
VTMVTLALLCGFGGGNFSSSMANISFFFPKSEKGTALGLNAGLGNLGVSIVQFVVPIVIT
HHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
MGLFGQLGGDPQHWVKGAAAKQMWLQNAGFVWVPFIVLSTLAAWFGMNDLASAKSSFSEQ
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
AVIFTRKHNWLMCWLYVGTFGSFIGFSAGFPLLVKGQFPDIDPTRYAFLGPLVGALTRPL
EEEEEECCCEEEEEEEHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
GGWLADKIGGAKVTQTVFALMVLGVLGVIFFLPHGGQGGNFYGFFAMFLCLFALTGVGNG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
STFMQVPAIFLTLHQRMAGPGESAMRQATQDATREAAAVLGFAGAIGAYGGFFIPKSYGT
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
SIAMTGGVEAALYLFIAFYLSCLAINGWYYARKNAEVRC
EEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]