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The map label for this gene is narI [H]

Identifier: 34497995

GI number: 34497995

Start: 2729983

End: 2730669

Strand: Reverse

Name: narI [H]

Synonym: CV_2540

Alternate gene names: 34497995

Gene position: 2730669-2729983 (Counterclockwise)

Preceding gene: 34497996

Following gene: 34497991

Centisome position: 57.47

GC content: 64.05

Gene sequence:

>687_bases
ATGGACTACCTGCATAGATTCGTATTCGGCATCTATCCCTACATCGCGCTGGCCATTTTCCTGCTGGGCAGCCTGATCCG
CTTCGAGCGCGAGCAGTACAGCTGGAAGAGCGAGTCGTCGCAGCTCTTGTACCGCGGCCAGCTGCGCCTGGGCAATATTC
TGTTCCACCTGGGCATCATCGGGCTGTTCTTCGGCCACCTAGTTGGTCTGTTGACGCCGCTGGCGGTGTGGGACGCGCTG
GGCGTGACGCACAACTTCAAGCAGGTGTTCGCCATGAGCGCCGGCGGCCTGATGGGCGGCCTGTGCCTGATCGGCATCGC
GCTGCTGCTGCACCGCCGCTTCCTATGCGACCGCCTGGCGGCCAACACCACTTGGCGCGACAAGCTGGTGCTGCTGTGGA
TACTGGCCACGCTGCTGCTGGGCCTGAGCACCATCGTCGTGTCGGCCCAGCATCTGGACGGTCACGAAATGGTGCTGCTG
ATGAGCTGGGCGCAGCATATCGTCACCTTCCGCGGCGACGCGGCGGATTTCATCGTCTCCGCCTCGCCGGTCTTCAAGCT
GCACCTGTTCATGGGCATGAGCCTGTTCGTGATCTTCCCCTTCACCCGGCTGGTGCATGTGTGGAGCGGCTTCGGCGCCG
TGACCTACCTGGGACGCGCCTGGCAGCTGGTGCGCCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
TTCTATCCGCGCGGCGCGCGCGCCTGAAACCTTACCCCCTCTTCGCTGAATGGGAGAGGGGCGGCGGACGGCGCTGACGC
TCCGCCATCGGGAGACAAAC

Downstream 100 bases:

>100_bases
ACGGCTGAACGGCTGGGCATGAAAAACGCCGCGCATCCTGTGAGGGGGCGCGGCGTTTTGATTAGGTAGACCACATTTTA
TTTCACGAGGAGTTCAGTTG

Product: nitrate reductase

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MDYLHRFVFGIYPYIALAIFLLGSLIRFEREQYSWKSESSQLLYRGQLRLGNILFHLGIIGLFFGHLVGLLTPLAVWDAL
GVTHNFKQVFAMSAGGLMGGLCLIGIALLLHRRFLCDRLAANTTWRDKLVLLWILATLLLGLSTIVVSAQHLDGHEMVLL
MSWAQHIVTFRGDAADFIVSASPVFKLHLFMGMSLFVIFPFTRLVHVWSGFGAVTYLGRAWQLVRPRG

Sequences:

>Translated_228_residues
MDYLHRFVFGIYPYIALAIFLLGSLIRFEREQYSWKSESSQLLYRGQLRLGNILFHLGIIGLFFGHLVGLLTPLAVWDAL
GVTHNFKQVFAMSAGGLMGGLCLIGIALLLHRRFLCDRLAANTTWRDKLVLLWILATLLLGLSTIVVSAQHLDGHEMVLL
MSWAQHIVTFRGDAADFIVSASPVFKLHLFMGMSLFVIFPFTRLVHVWSGFGAVTYLGRAWQLVRPRG
>Mature_228_residues
MDYLHRFVFGIYPYIALAIFLLGSLIRFEREQYSWKSESSQLLYRGQLRLGNILFHLGIIGLFFGHLVGLLTPLAVWDAL
GVTHNFKQVFAMSAGGLMGGLCLIGIALLLHRRFLCDRLAANTTWRDKLVLLWILATLLLGLSTIVVSAQHLDGHEMVLL
MSWAQHIVTFRGDAADFIVSASPVFKLHLFMGMSLFVIFPFTRLVHVWSGFGAVTYLGRAWQLVRPRG

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=48.8986784140969, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1787738, Length=227, Percent_Identity=46.6960352422907, Blast_Score=191, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25720; Mature: 25720

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLHRFVFGIYPYIALAIFLLGSLIRFEREQYSWKSESSQLLYRGQLRLGNILFHLGII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHH
GLFFGHLVGLLTPLAVWDALGVTHNFKQVFAMSAGGLMGGLCLIGIALLLHRRFLCDRLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ANTTWRDKLVLLWILATLLLGLSTIVVSAQHLDGHEMVLLMSWAQHIVTFRGDAADFIVS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCC
ASPVFKLHLFMGMSLFVIFPFTRLVHVWSGFGAVTYLGRAWQLVRPRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDYLHRFVFGIYPYIALAIFLLGSLIRFEREQYSWKSESSQLLYRGQLRLGNILFHLGII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHH
GLFFGHLVGLLTPLAVWDALGVTHNFKQVFAMSAGGLMGGLCLIGIALLLHRRFLCDRLA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ANTTWRDKLVLLWILATLLLGLSTIVVSAQHLDGHEMVLLMSWAQHIVTFRGDAADFIVS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCC
ASPVFKLHLFMGMSLFVIFPFTRLVHVWSGFGAVTYLGRAWQLVRPRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]