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The map label for this gene is purF [H]

Identifier: 34497970

GI number: 34497970

Start: 2707531

End: 2709060

Strand: Reverse

Name: purF [H]

Synonym: CV_2515

Alternate gene names: 34497970

Gene position: 2709060-2707531 (Counterclockwise)

Preceding gene: 34497971

Following gene: 34497969

Centisome position: 57.02

GC content: 64.97

Gene sequence:

>1530_bases
ATGTGTGGAATTCTAGGCGTGGTCGGTCAGTCGCCGGTCAATCAGCTGCTGTACGACGGTTTGCAGGTGTTGCAACATCG
CGGACAGGACGCGGCCGGTATCGTTACCGCCAATGGCAAGACCTTTCACATGCACAAAGGCAGCGGCATGGTGCGCGACG
TGTTCCGCACCCGCAACATGCGTTCGCTGCTGGGCAACGCCGGCATCGCCCACGTGCGCTACCCGACGGCCGGCTCGGCC
TCCTGCCTGGCGGAGGCCCAGCCGTTCTACGTCAACTCGCCCTTCGGCATCGTGCTGGCGCACAACGGCAACCTGACCAA
TACCGATGAGCTGAAGGCGGACATGTTCCGCAACGACCTCAGGCACATCAACACCAATTCGGACTCCGAAGTGCTGCTGA
ACGTGTTCGCCCACGAAGTGGCTCAACGCGTGACGGGCCACGAGCTGACTGTGGACGCGGTGTTCGGCGCGGTGGCCGCC
GTGCATCGCCGCGTCAAGGGCGCTTACGCGGTGGTGGCGATGATCGCCGGCTTCGGCCTGGTGGCCTTCCGCGACCCCAA
CGGCATCCGCCCGCTGGTGATGGGCTGCACCGAGACCGACGGCAAGACCGAGTACATGTTCGCGTCCGAGTCGGTGGCGC
TGGACTGTTCGGGCTTTTCGCTGCTGCGCGATGTGCAGCCGGGCGAGTGCGTGTACGTGACCTTCGACGGCGACATGCAC
AGCCAGGTCTGCGCCGAAAACACCCGTCTGGCGCCCTGCCTGTTCGAATACGTGTACTTCGCCCGCCCGGATTCGGTGAT
CGACGGCGTGTCGGTCTACCAGTCGCGCCTGAACATGGGCGAGACGCTGGCGGAGAAGATCCGCCGCGATCTGCCGGGCC
TGGACATCGACGTGGTGATCCCGATTCCCGACTCCAGCCGCCAGAGCGCGCTGCAACTTGCCAACGCGCTGGGCCTGCCG
TACCGCGAGGGCTTCATCAAGAACCGCTACATCGGCCGAACCTTCATCATGCCCGGCCAGGCGGTGCGCAGGAAATCGGT
GCGCCAGAAGCTGAACCCGGTGCCGCTGGAGTTCGCCGGCCGCAACGTGCTGCTGGTGGACGATTCCATCGTCCGCGGCA
CCACGTCCAAGGAAATCGTGCAGATGGCGCGCGATTCCGGCGCCAAGAAGGTCTATTTCGCGTCGGCCGCGCCGGCGGTG
CGCTTCCCCAATGTCTACGGCATCGACATGCCCACCCGGGCGGAGCTGCTGGCCACCGGCCGCGACGAGGCCGAGATCGC
GCGCGAGATCGGCGCCGACGCGGTGATTTACCAGGACCTGGCCGCGCTGAAGGATGCGGTGAGCAGCGTCAACCGCGAGC
TGAAGCTGTTCGAGAGTTCCTGCTTCGACGGCGAATACATCACCGGCGACATCACCGCGGCCTACCTGGACGCCATCGAG
TGCGCGCGGCTGGACCTGAAACACAAGGAAGACGGCAGCGAGCAGATGATCGATCTCAACCTGAACGTGGCGGAGCAGAA
TCTGATCTGA

Upstream 100 bases:

>100_bases
GGTGGAAGAGGCGGAATATCTTGATATCTTTACTTCAAGATTCCGCCTTTTTCTTTTGGGCTTTTCCTTTTTCAGCAAGT
TGGGATAGAGGGCATTAAAG

Downstream 100 bases:

>100_bases
GTCCGCTTCCGGCCTGGACTATCCTGAATAGGTTCCGGCCGGTCTGACCCCAACGGGGACGCTCCTGCGCCGGAGCGTCC
CCGTTTTCTTTTTGGAGAGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MCGILGVVGQSPVNQLLYDGLQVLQHRGQDAAGIVTANGKTFHMHKGSGMVRDVFRTRNMRSLLGNAGIAHVRYPTAGSA
SCLAEAQPFYVNSPFGIVLAHNGNLTNTDELKADMFRNDLRHINTNSDSEVLLNVFAHEVAQRVTGHELTVDAVFGAVAA
VHRRVKGAYAVVAMIAGFGLVAFRDPNGIRPLVMGCTETDGKTEYMFASESVALDCSGFSLLRDVQPGECVYVTFDGDMH
SQVCAENTRLAPCLFEYVYFARPDSVIDGVSVYQSRLNMGETLAEKIRRDLPGLDIDVVIPIPDSSRQSALQLANALGLP
YREGFIKNRYIGRTFIMPGQAVRRKSVRQKLNPVPLEFAGRNVLLVDDSIVRGTTSKEIVQMARDSGAKKVYFASAAPAV
RFPNVYGIDMPTRAELLATGRDEAEIAREIGADAVIYQDLAALKDAVSSVNRELKLFESSCFDGEYITGDITAAYLDAIE
CARLDLKHKEDGSEQMIDLNLNVAEQNLI

Sequences:

>Translated_509_residues
MCGILGVVGQSPVNQLLYDGLQVLQHRGQDAAGIVTANGKTFHMHKGSGMVRDVFRTRNMRSLLGNAGIAHVRYPTAGSA
SCLAEAQPFYVNSPFGIVLAHNGNLTNTDELKADMFRNDLRHINTNSDSEVLLNVFAHEVAQRVTGHELTVDAVFGAVAA
VHRRVKGAYAVVAMIAGFGLVAFRDPNGIRPLVMGCTETDGKTEYMFASESVALDCSGFSLLRDVQPGECVYVTFDGDMH
SQVCAENTRLAPCLFEYVYFARPDSVIDGVSVYQSRLNMGETLAEKIRRDLPGLDIDVVIPIPDSSRQSALQLANALGLP
YREGFIKNRYIGRTFIMPGQAVRRKSVRQKLNPVPLEFAGRNVLLVDDSIVRGTTSKEIVQMARDSGAKKVYFASAAPAV
RFPNVYGIDMPTRAELLATGRDEAEIAREIGADAVIYQDLAALKDAVSSVNRELKLFESSCFDGEYITGDITAAYLDAIE
CARLDLKHKEDGSEQMIDLNLNVAEQNLI
>Mature_509_residues
MCGILGVVGQSPVNQLLYDGLQVLQHRGQDAAGIVTANGKTFHMHKGSGMVRDVFRTRNMRSLLGNAGIAHVRYPTAGSA
SCLAEAQPFYVNSPFGIVLAHNGNLTNTDELKADMFRNDLRHINTNSDSEVLLNVFAHEVAQRVTGHELTVDAVFGAVAA
VHRRVKGAYAVVAMIAGFGLVAFRDPNGIRPLVMGCTETDGKTEYMFASESVALDCSGFSLLRDVQPGECVYVTFDGDMH
SQVCAENTRLAPCLFEYVYFARPDSVIDGVSVYQSRLNMGETLAEKIRRDLPGLDIDVVIPIPDSSRQSALQLANALGLP
YREGFIKNRYIGRTFIMPGQAVRRKSVRQKLNPVPLEFAGRNVLLVDDSIVRGTTSKEIVQMARDSGAKKVYFASAAPAV
RFPNVYGIDMPTRAELLATGRDEAEIAREIGADAVIYQDLAALKDAVSSVNRELKLFESSCFDGEYITGDITAAYLDAIE
CARLDLKHKEDGSEQMIDLNLNVAEQNLI

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=36.4210526315789, Blast_Score=266, Evalue=4e-71,
Organism=Escherichia coli, GI1788651, Length=487, Percent_Identity=60.5749486652977, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1790167, Length=218, Percent_Identity=30.2752293577982, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=494, Percent_Identity=35.2226720647773, Blast_Score=246, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6323958, Length=509, Percent_Identity=51.6699410609037, Blast_Score=489, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24659598, Length=497, Percent_Identity=35.010060362173, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24659604, Length=473, Percent_Identity=35.9408033826639, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI28573187, Length=505, Percent_Identity=34.0594059405941, Blast_Score=257, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55562; Mature: 55562

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGILGVVGQSPVNQLLYDGLQVLQHRGQDAAGIVTANGKTFHMHKGSGMVRDVFRTRNM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHHH
RSLLGNAGIAHVRYPTAGSASCLAEAQPFYVNSPFGIVLAHNGNLTNTDELKADMFRNDL
HHHHCCCCEEEEECCCCCCCHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVFAHEVAQRVTGHELTVDAVFGAVAAVHRRVKGAYAVVAMIAGFGL
HHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VAFRDPNGIRPLVMGCTETDGKTEYMFASESVALDCSGFSLLRDVQPGECVYVTFDGDMH
EEEECCCCCCEEEEECCCCCCCEEEEEECCCEEEECCCHHHHHCCCCCCEEEEEECCCCH
SQVCAENTRLAPCLFEYVYFARPDSVIDGVSVYQSRLNMGETLAEKIRRDLPGLDIDVVI
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEE
PIPDSSRQSALQLANALGLPYREGFIKNRYIGRTFIMPGQAVRRKSVRQKLNPVPLEFAG
ECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECC
RNVLLVDDSIVRGTTSKEIVQMARDSGAKKVYFASAAPAVRFPNVYGIDMPTRAELLATG
CEEEEECCCHHCCCCHHHHHHHHHCCCCCEEEEECCCCCEECCCEECCCCCCHHHHHHCC
RDEAEIAREIGADAVIYQDLAALKDAVSSVNRELKLFESSCFDGEYITGDITAAYLDAIE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
CARLDLKHKEDGSEQMIDLNLNVAEQNLI
HHHHCCCCCCCCCCEEEEEECCHHHCCCC
>Mature Secondary Structure
MCGILGVVGQSPVNQLLYDGLQVLQHRGQDAAGIVTANGKTFHMHKGSGMVRDVFRTRNM
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCCHHHHHHHHHHH
RSLLGNAGIAHVRYPTAGSASCLAEAQPFYVNSPFGIVLAHNGNLTNTDELKADMFRNDL
HHHHCCCCEEEEECCCCCCCHHHHCCCCEEEECCCEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVFAHEVAQRVTGHELTVDAVFGAVAAVHRRVKGAYAVVAMIAGFGL
HHCCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VAFRDPNGIRPLVMGCTETDGKTEYMFASESVALDCSGFSLLRDVQPGECVYVTFDGDMH
EEEECCCCCCEEEEECCCCCCCEEEEEECCCEEEECCCHHHHHCCCCCCEEEEEECCCCH
SQVCAENTRLAPCLFEYVYFARPDSVIDGVSVYQSRLNMGETLAEKIRRDLPGLDIDVVI
HHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEE
PIPDSSRQSALQLANALGLPYREGFIKNRYIGRTFIMPGQAVRRKSVRQKLNPVPLEFAG
ECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCEEECC
RNVLLVDDSIVRGTTSKEIVQMARDSGAKKVYFASAAPAVRFPNVYGIDMPTRAELLATG
CEEEEECCCHHCCCCHHHHHHHHHCCCCCEEEEECCCCCEECCCEECCCCCCHHHHHHCC
RDEAEIAREIGADAVIYQDLAALKDAVSSVNRELKLFESSCFDGEYITGDITAAYLDAIE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
CARLDLKHKEDGSEQMIDLNLNVAEQNLI
HHHHCCCCCCCCCCEEEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]