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The map label for this gene is dnaB [H]

Identifier: 34497957

GI number: 34497957

Start: 2693681

End: 2695087

Strand: Reverse

Name: dnaB [H]

Synonym: CV_2502

Alternate gene names: 34497957

Gene position: 2695087-2693681 (Counterclockwise)

Preceding gene: 34497958

Following gene: 34497956

Centisome position: 56.73

GC content: 66.88

Gene sequence:

>1407_bases
ATGACAGAACAGTACGAGAATTTCGACAGCGAGATGGCGGCCATCCGCACACCGCCGCATTCGGTCGAGGCCGAACAGTC
GGTGCTGGGCGGCCTGCTGCTGGACAACAGCGCCTGGGACAAGATCGCCGACGTGGTGTCCGAGGCCGACTTCTACCGCC
ACGACCATCGCCTGGTGTACAAGCAGATCGCCAAGCTGGTGGAGATGGCGCGCCCGGCCGACGTGGTGACGGTGGCCGAG
GCGCTGGACAAGAACGCCGAACTGGCCGAGATCGGCGGCTTGGCCTATCTGGCCACGCTGGCGCAGAACACGCCGTCGGC
GGCCAACATCCGCCGCTACGCCGAGATCGTCCGCGAGCGCTCGGTGATGCGGCAGCTGGCCCAGGTCGGCACCGAGATCG
CCGAGGCCGCCTACGCGCCGATGGGCCGCGACGCCGCGCAACTGCTGGACGAGGCCGAAGGCCGCGTGTTCCAGATCGCC
GAATCCACCGCCAAGTCCAAGCAAGGCTTTCTGGAAATGCCGGGCCTGCTGAAGGAAGTGGTGGAGCGGATCGACATGCT
TTACAGCCGGGACAACCCGGACGAAGTGACCGGCGTGCCGACCGGCTTCATCGATCTGGACGCCAAGACCTCCGGCCTGC
AGCCGGGCGACCTGATCATCGTCGCCGGCCGTCCGTCGATGGGTAAAACCGCCTTTTCGATGAACATCGCCGAGCACGTG
GCGGTGGAGACCCACCTGCCGGTGGCGGTGTTCTCGATGGAAATGGGCGGCGCGCAGCTGGTGATGCGTATGCTGGGCTC
GGTGGGCCGGCTGGATCAGCATGTGCTGCGCACCGGCAAGCTGGGCGACGACGACTGGCAGAAACTCACCTACGCGATCG
GCAAGCTGTCCGACGCGCCGATGTACATCGACGAAACCCCGGCGCTGACCGCGCTGGAACTGCGCGCCCGAGCCCGACGC
CTGGCCCGCCAGCACGGCGGCAAGCTTGGGCTTATCGTGATCGACTACTTGCAGCTGATGTCGGGCTCCGGCCGCGGCGA
CAACCGCACGGCCGAGCTGGGCGAAATCTCCCGCGGCCTGAAGGGCCTGGCCAAGGAGTTGCAGGTGCCGGTGATCGCCC
TGTCGCAGTTGTCGCGGGCGGTGGAGCAGCGGCCCAACAAGCGGCCGATGATGTCCGACCTTCGCGAATCGGGCGCCATC
GAGCAGGACGCCGACTTGATCATCTTCATGTACCGCGACGAGTACTACAATCCGGATTCGCCCGACAAGGGCCTGGCCGA
GGCCATCATCGGCAAGCACCGTAACGGCCCGACCGGCGCGGTGCGGCTGGCCTTCATCGGCCATTACGCCAAGTTCGACA
ACGCCGCCAGCGTCGGCGCGGCCGGCTGGGCGGACAGCGACAGCTAA

Upstream 100 bases:

>100_bases
CTACGTGGCGGGGATGAACTGCTGAGCGGCCCGGTTTGTTTCGGGTTAGAATTGCGTTTTGCCTTACGCGCGCCTTTTCG
GGCGCGCCTTCCGGGTCTAC

Downstream 100 bases:

>100_bases
GCGAGACATTCCATGAGCTTTTTCGACAAGCACGTATTCATCTGCTGCAACCAACGCGATGCCTGCCAGGATTGCTGCAA
CAACCACGGCTCCAGCGCGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTEQYENFDSEMAAIRTPPHSVEAEQSVLGGLLLDNSAWDKIADVVSEADFYRHDHRLVYKQIAKLVEMARPADVVTVAE
ALDKNAELAEIGGLAYLATLAQNTPSAANIRRYAEIVRERSVMRQLAQVGTEIAEAAYAPMGRDAAQLLDEAEGRVFQIA
ESTAKSKQGFLEMPGLLKEVVERIDMLYSRDNPDEVTGVPTGFIDLDAKTSGLQPGDLIIVAGRPSMGKTAFSMNIAEHV
AVETHLPVAVFSMEMGGAQLVMRMLGSVGRLDQHVLRTGKLGDDDWQKLTYAIGKLSDAPMYIDETPALTALELRARARR
LARQHGGKLGLIVIDYLQLMSGSGRGDNRTAELGEISRGLKGLAKELQVPVIALSQLSRAVEQRPNKRPMMSDLRESGAI
EQDADLIIFMYRDEYYNPDSPDKGLAEAIIGKHRNGPTGAVRLAFIGHYAKFDNAASVGAAGWADSDS

Sequences:

>Translated_468_residues
MTEQYENFDSEMAAIRTPPHSVEAEQSVLGGLLLDNSAWDKIADVVSEADFYRHDHRLVYKQIAKLVEMARPADVVTVAE
ALDKNAELAEIGGLAYLATLAQNTPSAANIRRYAEIVRERSVMRQLAQVGTEIAEAAYAPMGRDAAQLLDEAEGRVFQIA
ESTAKSKQGFLEMPGLLKEVVERIDMLYSRDNPDEVTGVPTGFIDLDAKTSGLQPGDLIIVAGRPSMGKTAFSMNIAEHV
AVETHLPVAVFSMEMGGAQLVMRMLGSVGRLDQHVLRTGKLGDDDWQKLTYAIGKLSDAPMYIDETPALTALELRARARR
LARQHGGKLGLIVIDYLQLMSGSGRGDNRTAELGEISRGLKGLAKELQVPVIALSQLSRAVEQRPNKRPMMSDLRESGAI
EQDADLIIFMYRDEYYNPDSPDKGLAEAIIGKHRNGPTGAVRLAFIGHYAKFDNAASVGAAGWADSDS
>Mature_467_residues
TEQYENFDSEMAAIRTPPHSVEAEQSVLGGLLLDNSAWDKIADVVSEADFYRHDHRLVYKQIAKLVEMARPADVVTVAEA
LDKNAELAEIGGLAYLATLAQNTPSAANIRRYAEIVRERSVMRQLAQVGTEIAEAAYAPMGRDAAQLLDEAEGRVFQIAE
STAKSKQGFLEMPGLLKEVVERIDMLYSRDNPDEVTGVPTGFIDLDAKTSGLQPGDLIIVAGRPSMGKTAFSMNIAEHVA
VETHLPVAVFSMEMGGAQLVMRMLGSVGRLDQHVLRTGKLGDDDWQKLTYAIGKLSDAPMYIDETPALTALELRARARRL
ARQHGGKLGLIVIDYLQLMSGSGRGDNRTAELGEISRGLKGLAKELQVPVIALSQLSRAVEQRPNKRPMMSDLRESGAIE
QDADLIIFMYRDEYYNPDSPDKGLAEAIIGKHRNGPTGAVRLAFIGHYAKFDNAASVGAAGWADSDS

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=449, Percent_Identity=55.011135857461, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51010; Mature: 50878

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQYENFDSEMAAIRTPPHSVEAEQSVLGGLLLDNSAWDKIADVVSEADFYRHDHRLVY
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KQIAKLVEMARPADVVTVAEALDKNAELAEIGGLAYLATLAQNTPSAANIRRYAEIVRER
HHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SVMRQLAQVGTEIAEAAYAPMGRDAAQLLDEAEGRVFQIAESTAKSKQGFLEMPGLLKEV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCCHHCCHHHHHH
VERIDMLYSRDNPDEVTGVPTGFIDLDAKTSGLQPGDLIIVAGRPSMGKTAFSMNIAEHV
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AVETHLPVAVFSMEMGGAQLVMRMLGSVGRLDQHVLRTGKLGDDDWQKLTYAIGKLSDAP
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
MYIDETPALTALELRARARRLARQHGGKLGLIVIDYLQLMSGSGRGDNRTAELGEISRGL
EEECCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KGLAKELQVPVIALSQLSRAVEQRPNKRPMMSDLRESGAIEQDADLIIFMYRDEYYNPDS
HHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
PDKGLAEAIIGKHRNGPTGAVRLAFIGHYAKFDNAASVGAAGWADSDS
CCHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TEQYENFDSEMAAIRTPPHSVEAEQSVLGGLLLDNSAWDKIADVVSEADFYRHDHRLVY
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KQIAKLVEMARPADVVTVAEALDKNAELAEIGGLAYLATLAQNTPSAANIRRYAEIVRER
HHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SVMRQLAQVGTEIAEAAYAPMGRDAAQLLDEAEGRVFQIAESTAKSKQGFLEMPGLLKEV
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCCHHCCHHHHHH
VERIDMLYSRDNPDEVTGVPTGFIDLDAKTSGLQPGDLIIVAGRPSMGKTAFSMNIAEHV
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AVETHLPVAVFSMEMGGAQLVMRMLGSVGRLDQHVLRTGKLGDDDWQKLTYAIGKLSDAP
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
MYIDETPALTALELRARARRLARQHGGKLGLIVIDYLQLMSGSGRGDNRTAELGEISRGL
EEECCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KGLAKELQVPVIALSQLSRAVEQRPNKRPMMSDLRESGAIEQDADLIIFMYRDEYYNPDS
HHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCC
PDKGLAEAIIGKHRNGPTGAVRLAFIGHYAKFDNAASVGAAGWADSDS
CCHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]