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The map label for this gene is nemR [H]

Identifier: 34497942

GI number: 34497942

Start: 2676417

End: 2677007

Strand: Reverse

Name: nemR [H]

Synonym: CV_2487

Alternate gene names: 34497942

Gene position: 2677007-2676417 (Counterclockwise)

Preceding gene: 34497944

Following gene: 34497941

Centisome position: 56.35

GC content: 62.94

Gene sequence:

>591_bases
ATGCATAATGGTTCCGACAGCAAGAACGACACCCGCAAGCGCATCCTCGACCTGGCCGAGGAGCTGTTGCTGACGCGCGG
CTTCAATGGCTTCAGTTATCAGCACATCAGCAGCGCGCTGGGCGTGCGCAACGCAGCCATCCACTACCACTTTCCCAAAA
AGAGCGATCTGGGCGTGGCGCTGATCCAGCGTTACCGCCGGCGTTTCCAGCGCTTCGTCGACCAGCAGGGGGAGTGGGAC
GCCGCTCGCCGCCTGGAGGCGTATTTCGGCCTGTCGGACCAGTATTACCTGCAGCACAAGCAAATCTGCCCCAGCGGCAT
TCTCAGCACCGAGTTCCAGACGTTGCCCGACGACATGCGGCAGGAAGCCTCCGCCTTCATCGACGAGATGAGGCGATGGG
CGGTCGCCATCGTCAGGCTGGGGCGGGAAAGCGGCCAGATGGATTACGCCGGTTCGCCCGAGGCGGTGGGGATGATGCTG
TTTTCCGCGCTGCAGGGCGGCCTGCAGTTGGCGCGCATTGACGAGGATGCGCTTCCGCTGCTGAAAAGCCAGGTCAGAAC
GACGCTGGGCATGGCCGAGACGCAGGAATAG

Upstream 100 bases:

>100_bases
GAGTGTCGCTTGTATTTTAAGTCATGCGCCGTTCCAGCGGGCAAGAAGCGCGGCGGGGCGGCCGATTGACACCTTACTGA
CCAGTAAGTAGAGTGGCCCG

Downstream 100 bases:

>100_bases
GCCGCCGGCCCGCCGGCGCAGACAACACCAAAACAACAAAGGGGGGTGCATGGAGTACAGAAAAAACAGCATGAGCCGGA
TCGCCGACAAGGTGGGCCGT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MHNGSDSKNDTRKRILDLAEELLLTRGFNGFSYQHISSALGVRNAAIHYHFPKKSDLGVALIQRYRRRFQRFVDQQGEWD
AARRLEAYFGLSDQYYLQHKQICPSGILSTEFQTLPDDMRQEASAFIDEMRRWAVAIVRLGRESGQMDYAGSPEAVGMML
FSALQGGLQLARIDEDALPLLKSQVRTTLGMAETQE

Sequences:

>Translated_196_residues
MHNGSDSKNDTRKRILDLAEELLLTRGFNGFSYQHISSALGVRNAAIHYHFPKKSDLGVALIQRYRRRFQRFVDQQGEWD
AARRLEAYFGLSDQYYLQHKQICPSGILSTEFQTLPDDMRQEASAFIDEMRRWAVAIVRLGRESGQMDYAGSPEAVGMML
FSALQGGLQLARIDEDALPLLKSQVRTTLGMAETQE
>Mature_196_residues
MHNGSDSKNDTRKRILDLAEELLLTRGFNGFSYQHISSALGVRNAAIHYHFPKKSDLGVALIQRYRRRFQRFVDQQGEWD
AARRLEAYFGLSDQYYLQHKQICPSGILSTEFQTLPDDMRQEASAFIDEMRRWAVAIVRLGRESGQMDYAGSPEAVGMML
FSALQGGLQLARIDEDALPLLKSQVRTTLGMAETQE

Specific function: Represses the transcription of the nemRA operon by binding to the nemR box [H]

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693141, Length=183, Percent_Identity=28.4153005464481, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR001647 [H]

Pfam domain/function: PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 22319; Mature: 22319

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNGSDSKNDTRKRILDLAEELLLTRGFNGFSYQHISSALGVRNAAIHYHFPKKSDLGVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHH
LIQRYRRRFQRFVDQQGEWDAARRLEAYFGLSDQYYLQHKQICPSGILSTEFQTLPDDMR
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHH
QEASAFIDEMRRWAVAIVRLGRESGQMDYAGSPEAVGMMLFSALQGGLQLARIDEDALPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHH
LKSQVRTTLGMAETQE
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHNGSDSKNDTRKRILDLAEELLLTRGFNGFSYQHISSALGVRNAAIHYHFPKKSDLGVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCHHHH
LIQRYRRRFQRFVDQQGEWDAARRLEAYFGLSDQYYLQHKQICPSGILSTEFQTLPDDMR
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCHHHH
QEASAFIDEMRRWAVAIVRLGRESGQMDYAGSPEAVGMMLFSALQGGLQLARIDEDALPL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHH
LKSQVRTTLGMAETQE
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]