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The map label for this gene is acnB [H]

Identifier: 34497925

GI number: 34497925

Start: 2655475

End: 2658063

Strand: Reverse

Name: acnB [H]

Synonym: CV_2470

Alternate gene names: 34497925

Gene position: 2658063-2655475 (Counterclockwise)

Preceding gene: 34497927

Following gene: 34497924

Centisome position: 55.95

GC content: 64.89

Gene sequence:

>2589_bases
ATGCTAGAAGCTTACCGTCAGCACGTTGCCGAGCGCGCCGCGCTGGGCATTCCGCCCCTGCCGCTGTCCGCCAAGCAGGT
TGAAGAACTGGTCGAACTGCTGAAGAATCCGCCCAAGGGCGAGGAAGCCACGCTGGTCGAACTGATCACCCACCGCGTGC
CGCCGGGCGTGGACGACGCCGCCAAGGTGAAGGCCTCCTTCCTGGCCGCCGTGGCCGAGGGCAGCGCCAAGTCGCCGCTG
GTTTCGCGCGAGCTGGCCACCGAATTGCTCGGCACCATGCTGGGCGGCTACAACATCAAGCCGCTGATCGACTTGCTGGA
CGACCCCGCCGTCGCCAAGATCGCCGCCGCCGGCCTGAAGAAAACCCTGCTGATGTTCGATTCCTTCCACGACGTGAAGG
AAAAGATGGACAAGGGCAACGCCGTCGCCAAGGAAGTGATCGAATCCTGGGCCAATGCCGAGTGGTTCACCAGCCGCCCT
GAAGTGGCCGAGAAAATCACCGTCACCGTGTTCAAGGTGCCTGGCGAAACCAATACCGACGACCTGTCGCCGGCCCCGGA
CGCCTGGAGCCGCCCGGACATCCCGCTGCACTACCTGGCCATGCTGAAGAACACCCGCGCCGACGCGGCCTTCAAGCCGG
AAGAGGACGGCAAGCGCGGCCCGATGCAGTTCATCGACGATCTGAAGAAGAAGGGCCATCTGGTCGCCTACGTCGGCGAT
GTGGTCGGCACCGGCTCCTCGCGCAAGTCCGCCACCAACTCGGTGGTGTGGGCCACCGGCCAGGACATCCCCTTCGTGCC
GAACAAGCGCTTTGGCGGCGTGACGCTGGGCGGCAAGATCGCCCCCATCTTCTTCAACACCCAGGAAGATTCCGGCTCGC
TGCCGATCGAAGTGGACGTGTCCAAGCTGGAAATGGGCGACGTGATCGACGTCTACCCGTACGCCGGCAAGATCGAGAAG
AACGGCGCCGTGGTGGCCGAGTTCAAGCTGAAGTCCGAAGTGATCCTGGATGAAGTGCGCGCCGGCGGCCGCATCAACCT
GATCATCGGCCGCGGCCTGACCGCCAAGGCGCGCGAAGCGCTGGGCCTGCCGGCTTCCACCGCATTCCGCCTGCCCAAGT
CCCCGGTCGATTCCGGCAAGGGCTTCTCGCTGGCGCAAAAGATGGTCGGCCGCGCCTGTGGCCTGCCGGAAGGCAAGGGC
GTGCGTCCGGGCACCTACTGCGAACCGAAGATGACCACCGTCGGCTCGCAGGACACTACCGGCCCGATGACCCGCGACGA
GCTGAAAGACCTGGCCTGCCTGGGCTTCTCCGCCGACCTGGTGATGCAATCGTTCTGCCACACCGCCGCCTATCCGAAGC
CGGTGGATGTGAAGATGCACAAGGAACTGCCGGCCTTCATCTCCACCCGCGGCGGGGTGTCGCTGCGTCCGGGCGACGGC
GTGATCCACAGCTGGCTGAACCGCCTGCTGCTGCCGGATACCGTCGGCACCGGCGGCGACAGCCACACCCGTTTCCCGAT
CGGCATTTCCTTCCCGGCCGGCTCCGGCTTGGTGGCCTTCGCCGCCGCCACCGGCGTGATGCCGCTGGACATGCCGGAGT
CGGTGCTGGTGCGCTTCAAGGGCAAGCTGCAGCCGGGCGTCACCCTGCGCGACCTGGTCAACGCCATCCCCTTGTACGCG
ATCAAGCAAGGCCTGCTGACCGTGGCCAAGGCCGGCAAGAAGAACATCTTCTCCGGCCGCATCCTGGAAATCGAAGGCCT
GCCGGATCTGAAGGTCGAGCAAGCGTTCGAGCTGACCGACGCCTCGGCCGAGCGTTCCGCCGCCGGCTGCACCGTGCACC
TGAACAAGGAGCCGATCGTCGAATACATGCGCTCCAACATCACGCTGATGAAGGCGATGATCGCCGACGGTTACCAGGAC
GCGCGCACGCTGGAACGCCGCATCAAGTCGATGGAAGACTGGATCGCCAACGGCCAGCTGCTGAAGGGCGATGCCGACGC
CGAATACGCGGCGGTGATCGAGATCGACATGAACGAGATCAAGGAGCCCATCGTCGCCTGCCCGAACGACCCGGACGATG
TGAAGCTGATGTCCGAAGTGTCCGGCACCAAGATCGACGAAGTGTTCATCGGCTCGTGCATGACCAATATCGGCCACTTC
CGCGCCGCGTCCAAGCTGCTGGAAGGCAAGGCGGATATCCCGGTGCGCCTGTGGGTGGCCCCGCCGACCAAGATGGACGC
CAAGCAGCTGACCGAGGAAGGCCACTACGGCGTGCTGGGCCGCGCCGGCGCCCGCCTGGAAATGCCGGGCTGCTCGCTGT
GCATGGGCAACCAGGCGCAGGTGCGCGAGGGCGCGACCGTGATGTCCACCTCCACCCGCAACTTCCCCAACCGCCTGGGC
AAGAACTCCAATGTGTTCCTGGGTTCCGCCGAGCTGGCCGCGATCTGCTCCAAGCTGGGCAAGATCCCGACCGTGGAGGA
GTACCAGTCCAACATCGGCATCATCAACGAGAAAGGCGCCGAAGTTTACAAGTACCTGAACTTCGATCAAATCGCTGAAT
ATCAGGAGCTGGCCGCCAGCAAGGCCTGA

Upstream 100 bases:

>100_bases
TATCCCGCAGCGAGAGTGCGGGATGGTGCGAAATCAGCAAGTGGAAGCCGCCGGCCCGCGCCGGCAGCCTGCGAAACCAA
AGCCAAGAAGGTCCCGAGTC

Downstream 100 bases:

>100_bases
TAGCCGAACCGATTACCTTGGTGATGGCGCCGTTCGCCCGCATGGGCGGGCGGCGTTTTTTTTGCGGGAACTGGCGAGGC
GTTTTTCGGCCCAATCCGGG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MLEAYRQHVAERAALGIPPLPLSAKQVEELVELLKNPPKGEEATLVELITHRVPPGVDDAAKVKASFLAAVAEGSAKSPL
VSRELATELLGTMLGGYNIKPLIDLLDDPAVAKIAAAGLKKTLLMFDSFHDVKEKMDKGNAVAKEVIESWANAEWFTSRP
EVAEKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNTRADAAFKPEEDGKRGPMQFIDDLKKKGHLVAYVGD
VVGTGSSRKSATNSVVWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDVSKLEMGDVIDVYPYAGKIEK
NGAVVAEFKLKSEVILDEVRAGGRINLIIGRGLTAKAREALGLPASTAFRLPKSPVDSGKGFSLAQKMVGRACGLPEGKG
VRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKMHKELPAFISTRGGVSLRPGDG
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGVTLRDLVNAIPLYA
IKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVHLNKEPIVEYMRSNITLMKAMIADGYQD
ARTLERRIKSMEDWIANGQLLKGDADAEYAAVIEIDMNEIKEPIVACPNDPDDVKLMSEVSGTKIDEVFIGSCMTNIGHF
RAASKLLEGKADIPVRLWVAPPTKMDAKQLTEEGHYGVLGRAGARLEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNSNVFLGSAELAAICSKLGKIPTVEEYQSNIGIINEKGAEVYKYLNFDQIAEYQELAASKA

Sequences:

>Translated_862_residues
MLEAYRQHVAERAALGIPPLPLSAKQVEELVELLKNPPKGEEATLVELITHRVPPGVDDAAKVKASFLAAVAEGSAKSPL
VSRELATELLGTMLGGYNIKPLIDLLDDPAVAKIAAAGLKKTLLMFDSFHDVKEKMDKGNAVAKEVIESWANAEWFTSRP
EVAEKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNTRADAAFKPEEDGKRGPMQFIDDLKKKGHLVAYVGD
VVGTGSSRKSATNSVVWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDVSKLEMGDVIDVYPYAGKIEK
NGAVVAEFKLKSEVILDEVRAGGRINLIIGRGLTAKAREALGLPASTAFRLPKSPVDSGKGFSLAQKMVGRACGLPEGKG
VRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKMHKELPAFISTRGGVSLRPGDG
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGVTLRDLVNAIPLYA
IKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVHLNKEPIVEYMRSNITLMKAMIADGYQD
ARTLERRIKSMEDWIANGQLLKGDADAEYAAVIEIDMNEIKEPIVACPNDPDDVKLMSEVSGTKIDEVFIGSCMTNIGHF
RAASKLLEGKADIPVRLWVAPPTKMDAKQLTEEGHYGVLGRAGARLEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNSNVFLGSAELAAICSKLGKIPTVEEYQSNIGIINEKGAEVYKYLNFDQIAEYQELAASKA
>Mature_862_residues
MLEAYRQHVAERAALGIPPLPLSAKQVEELVELLKNPPKGEEATLVELITHRVPPGVDDAAKVKASFLAAVAEGSAKSPL
VSRELATELLGTMLGGYNIKPLIDLLDDPAVAKIAAAGLKKTLLMFDSFHDVKEKMDKGNAVAKEVIESWANAEWFTSRP
EVAEKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNTRADAAFKPEEDGKRGPMQFIDDLKKKGHLVAYVGD
VVGTGSSRKSATNSVVWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDVSKLEMGDVIDVYPYAGKIEK
NGAVVAEFKLKSEVILDEVRAGGRINLIIGRGLTAKAREALGLPASTAFRLPKSPVDSGKGFSLAQKMVGRACGLPEGKG
VRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKMHKELPAFISTRGGVSLRPGDG
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKLQPGVTLRDLVNAIPLYA
IKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVHLNKEPIVEYMRSNITLMKAMIADGYQD
ARTLERRIKSMEDWIANGQLLKGDADAEYAAVIEIDMNEIKEPIVACPNDPDDVKLMSEVSGTKIDEVFIGSCMTNIGHF
RAASKLLEGKADIPVRLWVAPPTKMDAKQLTEEGHYGVLGRAGARLEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNSNVFLGSAELAAICSKLGKIPTVEEYQSNIGIINEKGAEVYKYLNFDQIAEYQELAASKA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=861, Percent_Identity=73.5191637630662, Blast_Score=1267, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=374, Percent_Identity=24.8663101604278, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=370, Percent_Identity=25.1351351351351, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92755; Mature: 92755

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRQHVAERAALGIPPLPLSAKQVEELVELLKNPPKGEEATLVELITHRVPPGVDDA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AKVKASFLAAVAEGSAKSPLVSRELATELLGTMLGGYNIKPLIDLLDDPAVAKIAAAGLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHH
KTLLMFDSFHDVKEKMDKGNAVAKEVIESWANAEWFTSRPEVAEKITVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHYLAMLKNTRADAAFKPEEDGKRGPMQFIDDLKKKGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECH
VVGTGSSRKSATNSVVWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDV
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCCEEEEH
SKLEMGDVIDVYPYAGKIEKNGAVVAEFKLKSEVILDEVRAGGRINLIIGRGLTAKAREA
HHCCCCCEEEECCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LGLPASTAFRLPKSPVDSGKGFSLAQKMVGRACGLPEGKGVRPGTYCEPKMTTVGSQDTT
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKMHKELPAFISTRGGVSLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHHCCHHHHCCCCCEECCCCC
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCC
GKLQPGVTLRDLVNAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCC
ASAERSAAGCTVHLNKEPIVEYMRSNITLMKAMIADGYQDARTLERRIKSMEDWIANGQL
CCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEE
LKGDADAEYAAVIEIDMNEIKEPIVACPNDPDDVKLMSEVSGTKIDEVFIGSCMTNIGHF
EECCCCCCEEEEEEECHHHHCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAASKLLEGKADIPVRLWVAPPTKMDAKQLTEEGHYGVLGRAGARLEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCEEECCCCCEECCCHHH
VREGATVMSTSTRNFPNRLGKNSNVFLGSAELAAICSKLGKIPTVEEYQSNIGIINEKGA
HHCCCEEEECCCCCHHHHHCCCCCEEECCHHHHHHHHHHCCCCCHHHHHCCCCEEECCCH
EVYKYLNFDQIAEYQELAASKA
HHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEAYRQHVAERAALGIPPLPLSAKQVEELVELLKNPPKGEEATLVELITHRVPPGVDDA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AKVKASFLAAVAEGSAKSPLVSRELATELLGTMLGGYNIKPLIDLLDDPAVAKIAAAGLK
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHH
KTLLMFDSFHDVKEKMDKGNAVAKEVIESWANAEWFTSRPEVAEKITVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHYLAMLKNTRADAAFKPEEDGKRGPMQFIDDLKKKGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECH
VVGTGSSRKSATNSVVWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDV
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCCEEEEH
SKLEMGDVIDVYPYAGKIEKNGAVVAEFKLKSEVILDEVRAGGRINLIIGRGLTAKAREA
HHCCCCCEEEECCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
LGLPASTAFRLPKSPVDSGKGFSLAQKMVGRACGLPEGKGVRPGTYCEPKMTTVGSQDTT
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKMHKELPAFISTRGGVSLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEHHHHCCHHHHCCCCCEECCCCC
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCC
GKLQPGVTLRDLVNAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELTD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCC
ASAERSAAGCTVHLNKEPIVEYMRSNITLMKAMIADGYQDARTLERRIKSMEDWIANGQL
CCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEE
LKGDADAEYAAVIEIDMNEIKEPIVACPNDPDDVKLMSEVSGTKIDEVFIGSCMTNIGHF
EECCCCCCEEEEEEECHHHHCCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAASKLLEGKADIPVRLWVAPPTKMDAKQLTEEGHYGVLGRAGARLEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCEEECCCCCEECCCHHH
VREGATVMSTSTRNFPNRLGKNSNVFLGSAELAAICSKLGKIPTVEEYQSNIGIINEKGA
HHCCCEEEECCCCCHHHHHCCCCCEEECCHHHHHHHHHHCCCCCHHHHHCCCCEEECCCH
EVYKYLNFDQIAEYQELAASKA
HHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]