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The map label for this gene is ilvB [H]

Identifier: 33863507

GI number: 33863507

Start: 1326056

End: 1327777

Strand: Reverse

Name: ilvB [H]

Synonym: PMT1239

Alternate gene names: 33863507

Gene position: 1327777-1326056 (Counterclockwise)

Preceding gene: 33863508

Following gene: 33863506

Centisome position: 55.07

GC content: 51.1

Gene sequence:

>1722_bases
ATGACGGGCGCCGATGCCCTGATGGATGCTCTTCGCCGCAACGGTGTGGAGATCATCTTTGGTTATCCAGGTGGTGCAAT
CCTTCCGATTTACGACGCCGTTTATAAGGCTGAGCAGCAGGGATGGTTGAAGCACATCCTTGTTCGCCATGAGCAGGGGG
GCGCCCATGCGGCTGATGGCTATGCAAGGGCTACAGGTCGTGTTGGAGTTTGCTTTGGAACCTCTGGGCCAGGCGCAACC
AATCTCGTTACTGGTATCGCCACGGCTCAGATGGATTCTGTTCCAATGGTGGTGATTACAGGACAGGTCCCTCGACCATG
CATTGGTACAGATGCTTTTCAGGAAACTGATATTTTCGGGATCACGCTGCCTATCGTGAAACACTCCTGGGTTGTTCGCG
ATCCGGGTGATTTGGCTTCGGTTGTTGCTCAAGCATTTTTTATTGCAGCTTCTGGGAGACCTGGTCCGGTGTTGATTGAT
ATCCCTAAGGATGTGGGCCAGGAGGAATTCGACTATCAGCCAGTTGAACCTGGATCGGTCGTGCCTGCAGGATTTCACCG
ATTCCCGGCTCCAGAACTTTCCTCAATTGAGGCGGCTCTCGATTTAATTGATGATGCTCAGAGGCCTCTCCTTTACGTTG
GCGGAGGAGTTATTTCTGCCGGGGCTCACGAGAGTCTTAAGGGTTTTGCAGAACGTCACCAGATACCTGTCACCACCACA
TTGATGGGTAAGGGGGCGTTTGATGAGCGACATCCTTTGGCTTTGGGGATGTTGGGAATGCATGGCACTGCCTATGCCAA
CTTTGCAGTTACTGAATGTGATTTGTTGATCGCTGTTGGTGCACGTTTCGATGACCGTGTTACAGGCAAGCTTGATACTT
TTGCGCCCCGTGCAAGAGTGATTCATTTTGAAATTGATCCAGCAGAAGTAGCTAAGAACCGACGTCCTGATGTTGCGGTA
TTGGGTGATGTTGGTGTCAGCTTGGTGAAGTTATTCGACTTGAGTAAGCAAAAGTCTGTTGAGTTGAGGACTTCAGCGTG
GCTAACTCGTATTGAGAGTTGGAAGAACCTTTACCCCTTGATGACTCCGCCTGAGGAGGGGCCAATTTATCCTCAGGAGG
TGCTCTTGGCGATAAGGGACTTGGCTCCTGAGGCCTACATAACAACTGATGTGGGCCAGCATCAGATGTGGGCGGCTCAA
TATTTACGGAATGGGCCTCGTCAGTGGATCAGTAGCGCGGGATTGGGAACGATGGGTTTCGGGATGCCAGCTGCTATTGG
TGTTCAAGTTGCTTTGCCAGATGAACAGGTTGTTTGTATCGCTGGTGATTCCAGCATCCTGATGAATATTCAGGAGTTGG
GAACACTGATTGAGTACAACATCCCAGCCAAGATTGTGATTGTGAACAACCACTGGCAAGGGATGGTTCGTCAGTGGCAG
CAAAGCTTCTACGAAGATCGTTATTCAGCCACTGACATGCTTCCGGGCATGCCTGATTTCGTGGCATTGGCGAAGGCCTT
TAGGGTTGGCGGGGTGTTGATCACTGAGCGAAAGGATCTCAGGTCAAGTTTGAAGCAGGCCCTTGCGACTCCAGGTCCGA
TGCTCATCGACGTGCATGTGCGACGTGATGAAAATTGCTATCCGATGGTTCCTCCTGGCAAGAGCAACGCTCAAATGGTG
GGTTTATCTAACCACCCTGAACTTTCTGGGGTTTCTTTGTGA

Upstream 100 bases:

>100_bases
GCCCTTAATCTTTAGGAGCAGATCATGCTTTGCCACTGCCGTGACTTTGACTTCTTCAACCACGGCTCTCGGTGGTTCAT
CACAACAAGTGCAAAAGCGT

Downstream 100 bases:

>100_bases
TGCAACGACTGCAGTTTGTTCTTCTAAGCATTCTGCTTGTTTTGTTCATCGCACCATTGCCTGTCATGGCGGCGGAAGTG
CTGCGTGTGAGTAGCTCCTC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MTGADALMDALRRNGVEIIFGYPGGAILPIYDAVYKAEQQGWLKHILVRHEQGGAHAADGYARATGRVGVCFGTSGPGAT
NLVTGIATAQMDSVPMVVITGQVPRPCIGTDAFQETDIFGITLPIVKHSWVVRDPGDLASVVAQAFFIAASGRPGPVLID
IPKDVGQEEFDYQPVEPGSVVPAGFHRFPAPELSSIEAALDLIDDAQRPLLYVGGGVISAGAHESLKGFAERHQIPVTTT
LMGKGAFDERHPLALGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDTFAPRARVIHFEIDPAEVAKNRRPDVAV
LGDVGVSLVKLFDLSKQKSVELRTSAWLTRIESWKNLYPLMTPPEEGPIYPQEVLLAIRDLAPEAYITTDVGQHQMWAAQ
YLRNGPRQWISSAGLGTMGFGMPAAIGVQVALPDEQVVCIAGDSSILMNIQELGTLIEYNIPAKIVIVNNHWQGMVRQWQ
QSFYEDRYSATDMLPGMPDFVALAKAFRVGGVLITERKDLRSSLKQALATPGPMLIDVHVRRDENCYPMVPPGKSNAQMV
GLSNHPELSGVSL

Sequences:

>Translated_573_residues
MTGADALMDALRRNGVEIIFGYPGGAILPIYDAVYKAEQQGWLKHILVRHEQGGAHAADGYARATGRVGVCFGTSGPGAT
NLVTGIATAQMDSVPMVVITGQVPRPCIGTDAFQETDIFGITLPIVKHSWVVRDPGDLASVVAQAFFIAASGRPGPVLID
IPKDVGQEEFDYQPVEPGSVVPAGFHRFPAPELSSIEAALDLIDDAQRPLLYVGGGVISAGAHESLKGFAERHQIPVTTT
LMGKGAFDERHPLALGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDTFAPRARVIHFEIDPAEVAKNRRPDVAV
LGDVGVSLVKLFDLSKQKSVELRTSAWLTRIESWKNLYPLMTPPEEGPIYPQEVLLAIRDLAPEAYITTDVGQHQMWAAQ
YLRNGPRQWISSAGLGTMGFGMPAAIGVQVALPDEQVVCIAGDSSILMNIQELGTLIEYNIPAKIVIVNNHWQGMVRQWQ
QSFYEDRYSATDMLPGMPDFVALAKAFRVGGVLITERKDLRSSLKQALATPGPMLIDVHVRRDENCYPMVPPGKSNAQMV
GLSNHPELSGVSL
>Mature_572_residues
TGADALMDALRRNGVEIIFGYPGGAILPIYDAVYKAEQQGWLKHILVRHEQGGAHAADGYARATGRVGVCFGTSGPGATN
LVTGIATAQMDSVPMVVITGQVPRPCIGTDAFQETDIFGITLPIVKHSWVVRDPGDLASVVAQAFFIAASGRPGPVLIDI
PKDVGQEEFDYQPVEPGSVVPAGFHRFPAPELSSIEAALDLIDDAQRPLLYVGGGVISAGAHESLKGFAERHQIPVTTTL
MGKGAFDERHPLALGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDTFAPRARVIHFEIDPAEVAKNRRPDVAVL
GDVGVSLVKLFDLSKQKSVELRTSAWLTRIESWKNLYPLMTPPEEGPIYPQEVLLAIRDLAPEAYITTDVGQHQMWAAQY
LRNGPRQWISSAGLGTMGFGMPAAIGVQVALPDEQVVCIAGDSSILMNIQELGTLIEYNIPAKIVIVNNHWQGMVRQWQQ
SFYEDRYSATDMLPGMPDFVALAKAFRVGGVLITERKDLRSSLKQALATPGPMLIDVHVRRDENCYPMVPPGKSNAQMVG
LSNHPELSGVSL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=577, Percent_Identity=27.5563258232236, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI21361361, Length=520, Percent_Identity=25.5769230769231, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI87081685, Length=559, Percent_Identity=46.1538461538462, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=45.4063604240283, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1786717, Length=571, Percent_Identity=34.3257443082312, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1787096, Length=573, Percent_Identity=27.5741710296684, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=28.5714285714286, Blast_Score=176, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=27.7296360485269, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=27.7296360485269, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=521, Percent_Identity=26.295585412668, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=45.1557093425605, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=22.4948875255624, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320588, Length=509, Percent_Identity=21.6110019646365, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=490, Percent_Identity=24.0816326530612, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922626, Length=562, Percent_Identity=26.8683274021352, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61933; Mature: 61802

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGADALMDALRRNGVEIIFGYPGGAILPIYDAVYKAEQQGWLKHILVRHEQGGAHAADG
CCCHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
YARATGRVGVCFGTSGPGATNLVTGIATAQMDSVPMVVITGQVPRPCIGTDAFQETDIFG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
ITLPIVKHSWVVRDPGDLASVVAQAFFIAASGRPGPVLIDIPKDVGQEEFDYQPVEPGSV
EEEEHHCCEEEEECCHHHHHHHHHHHEEEECCCCCCEEEECCHHCCCCCCCCCCCCCCCC
VPAGFHRFPAPELSSIEAALDLIDDAQRPLLYVGGGVISAGAHESLKGFAERHQIPVTTT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCHHHHHHHHHHCCCCEEEE
LMGKGAFDERHPLALGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDTFAPRARV
EECCCCCCCCCCEEEEEECCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHCCCCCEEE
IHFEIDPAEVAKNRRPDVAVLGDVGVSLVKLFDLSKQKSVELRTSAWLTRIESWKNLYPL
EEEECCHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCC
MTPPEEGPIYPQEVLLAIRDLAPEAYITTDVGQHQMWAAQYLRNGPRQWISSAGLGTMGF
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCC
GMPAAIGVQVALPDEQVVCIAGDSSILMNIQELGTLIEYNIPAKIVIVNNHWQGMVRQWQ
CCCHHEEEEEECCCCCEEEEECCCHHEEEHHHHCCEEEECCCEEEEEECCCHHHHHHHHH
QSFYEDRYSATDMLPGMPDFVALAKAFRVGGVLITERKDLRSSLKQALATPGPMLIDVHV
HHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCEEEEEEE
RRDENCYPMVPPGKSNAQMVGLSNHPELSGVSL
ECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TGADALMDALRRNGVEIIFGYPGGAILPIYDAVYKAEQQGWLKHILVRHEQGGAHAADG
CCHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
YARATGRVGVCFGTSGPGATNLVTGIATAQMDSVPMVVITGQVPRPCIGTDAFQETDIFG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
ITLPIVKHSWVVRDPGDLASVVAQAFFIAASGRPGPVLIDIPKDVGQEEFDYQPVEPGSV
EEEEHHCCEEEEECCHHHHHHHHHHHEEEECCCCCCEEEECCHHCCCCCCCCCCCCCCCC
VPAGFHRFPAPELSSIEAALDLIDDAQRPLLYVGGGVISAGAHESLKGFAERHQIPVTTT
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEECCCHHHHHHHHHHCCCCEEEE
LMGKGAFDERHPLALGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDTFAPRARV
EECCCCCCCCCCEEEEEECCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCHHCCCCCEEE
IHFEIDPAEVAKNRRPDVAVLGDVGVSLVKLFDLSKQKSVELRTSAWLTRIESWKNLYPL
EEEECCHHHHHHCCCCCEEEECHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCC
MTPPEEGPIYPQEVLLAIRDLAPEAYITTDVGQHQMWAAQYLRNGPRQWISSAGLGTMGF
CCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCCCCC
GMPAAIGVQVALPDEQVVCIAGDSSILMNIQELGTLIEYNIPAKIVIVNNHWQGMVRQWQ
CCCHHEEEEEECCCCCEEEEECCCHHEEEHHHHCCEEEECCCEEEEEECCCHHHHHHHHH
QSFYEDRYSATDMLPGMPDFVALAKAFRVGGVLITERKDLRSSLKQALATPGPMLIDVHV
HHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCEEEEEEE
RRDENCYPMVPPGKSNAQMVGLSNHPELSGVSL
ECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]