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The map label for this gene is BioB [H]

Identifier: 33863327

GI number: 33863327

Start: 1138944

End: 1139951

Strand: Reverse

Name: BioB [H]

Synonym: PMT1056

Alternate gene names: 33863327

Gene position: 1139951-1138944 (Counterclockwise)

Preceding gene: 33863328

Following gene: 33863326

Centisome position: 47.28

GC content: 53.97

Gene sequence:

>1008_bases
ATGACCGTGCTTATTGCCAAGGATCTCACTTTTGAACCCGTTGTTCATCGTTATGACTGGACAGTGGATGAGGTGCGGGA
ACTTTTGGAGCGACCACTTATGGACTTGCTCTGGCAGGCCCAGCTTGTGCATCGGACGGCAAATCCTGGCTATCGGGTCC
AGCTGGCTTCTTTGCTCAGCGTGAAGACGGGAGGATGCCAGGAGGATTGCGCTTATTGCCCCCAGTCCATGCACAACAGT
AGTGATGTTGAAGGGCAGCCCGATCTAGTGGTTCAGATCCAAACAGTGCTTGAGCGCGCACGGGCCGCCAAAGATGCTGG
TGCGGATCGCTTTTGCATGGGATGGGCTTGGCGGGAGATCAGAGATGGTGCCCAGTTTGAAGCCATGCTTGCGATGGTGA
GTGGTGTACGGGAGTTGGGACTAGAGGCCTGTGTTACTGCCGGCATGCTGACTGAAAAGCAGGCTTCTCGGTTGGCAGAT
GCGGGATTGACTGCCTACAACCACAATCTCGATACCAGCCCTGAGTACTACGACCAGATCATCACAACGCGCACATACCA
AGAGCGAATCGAAACCCTTCAGAAGGTTCGTTCAGCTGGCATCACTCTTTGCTGTGGGGGCATCATCGGGATGGGGGAAT
CAACTGTGGATCGAGCCTCTCTTCTTTGTGTACTAGCCAATATCAACCCTCATCCTGAGAGTGTTCCAATTAATGGGCTG
GTGGCAGTGGAGGGCACGCCATTGCAGGACCTACCTGCGGTGGATCCTTTAGAGATGGTGCGCATGGTTGCGACGGCTCG
AATTCTGATGCCTCGCAGTCGTGTCCGTCTAAGCGCAGGGCGTGAGCAGCTTGGTCGAGAGGCTCAGATCCTTTGTCTCC
AAGCGGGAGCTGATTCGATCTTTTACGGCGATAGTTTGCTAACTACCAGCAATCCTGATGTAAAAACTGATCGCGAACTT
CTCTCTCAGGCAGGAGTTCATGCGAATTGGGAGGAGAGTGACGAATGA

Upstream 100 bases:

>100_bases
AAGCTCTTTTTGATTACCAGTCACGCTGTCGTCGTTTCGGCGGTTTGGATCCAATCGCAGCGAATCATCTGGGATCTTGA
CCGTTGGCTCCAGTCGTTCC

Downstream 100 bases:

>100_bases
ATCTGCAAGATGATCAGACCTCTGCAGATCTATTTCAGGTAGCTACTTTCTATTCCTTTACTGCTTGGACTGAGGTCACT
ATTGCCTGCTTATTGCATGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTVLIAKDLTFEPVVHRYDWTVDEVRELLERPLMDLLWQAQLVHRTANPGYRVQLASLLSVKTGGCQEDCAYCPQSMHNS
SDVEGQPDLVVQIQTVLERARAAKDAGADRFCMGWAWREIRDGAQFEAMLAMVSGVRELGLEACVTAGMLTEKQASRLAD
AGLTAYNHNLDTSPEYYDQIITTRTYQERIETLQKVRSAGITLCCGGIIGMGESTVDRASLLCVLANINPHPESVPINGL
VAVEGTPLQDLPAVDPLEMVRMVATARILMPRSRVRLSAGREQLGREAQILCLQAGADSIFYGDSLLTTSNPDVKTDREL
LSQAGVHANWEESDE

Sequences:

>Translated_335_residues
MTVLIAKDLTFEPVVHRYDWTVDEVRELLERPLMDLLWQAQLVHRTANPGYRVQLASLLSVKTGGCQEDCAYCPQSMHNS
SDVEGQPDLVVQIQTVLERARAAKDAGADRFCMGWAWREIRDGAQFEAMLAMVSGVRELGLEACVTAGMLTEKQASRLAD
AGLTAYNHNLDTSPEYYDQIITTRTYQERIETLQKVRSAGITLCCGGIIGMGESTVDRASLLCVLANINPHPESVPINGL
VAVEGTPLQDLPAVDPLEMVRMVATARILMPRSRVRLSAGREQLGREAQILCLQAGADSIFYGDSLLTTSNPDVKTDREL
LSQAGVHANWEESDE
>Mature_334_residues
TVLIAKDLTFEPVVHRYDWTVDEVRELLERPLMDLLWQAQLVHRTANPGYRVQLASLLSVKTGGCQEDCAYCPQSMHNSS
DVEGQPDLVVQIQTVLERARAAKDAGADRFCMGWAWREIRDGAQFEAMLAMVSGVRELGLEACVTAGMLTEKQASRLADA
GLTAYNHNLDTSPEYYDQIITTRTYQERIETLQKVRSAGITLCCGGIIGMGESTVDRASLLCVLANINPHPESVPINGLV
AVEGTPLQDLPAVDPLEMVRMVATARILMPRSRVRLSAGREQLGREAQILCLQAGADSIFYGDSLLTTSNPDVKTDRELL
SQAGVHANWEESDE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=316, Percent_Identity=54.746835443038, Blast_Score=348, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=42.7672955974843, Blast_Score=284, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36889; Mature: 36758

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLIAKDLTFEPVVHRYDWTVDEVRELLERPLMDLLWQAQLVHRTANPGYRVQLASLLS
CEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
VKTGGCQEDCAYCPQSMHNSSDVEGQPDLVVQIQTVLERARAAKDAGADRFCMGWAWREI
HCCCCCHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RDGAQFEAMLAMVSGVRELGLEACVTAGMLTEKQASRLADAGLTAYNHNLDTSPEYYDQI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHH
ITTRTYQERIETLQKVRSAGITLCCGGIIGMGESTVDRASLLCVLANINPHPESVPINGL
HHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHHHEEEEECCCCCCCCCCCCEE
VAVEGTPLQDLPAVDPLEMVRMVATARILMPRSRVRLSAGREQLGREAQILCLQAGADSI
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCE
FYGDSLLTTSNPDVKTDRELLSQAGVHANWEESDE
EECCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TVLIAKDLTFEPVVHRYDWTVDEVRELLERPLMDLLWQAQLVHRTANPGYRVQLASLLS
EEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHH
VKTGGCQEDCAYCPQSMHNSSDVEGQPDLVVQIQTVLERARAAKDAGADRFCMGWAWREI
HCCCCCHHHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RDGAQFEAMLAMVSGVRELGLEACVTAGMLTEKQASRLADAGLTAYNHNLDTSPEYYDQI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHH
ITTRTYQERIETLQKVRSAGITLCCGGIIGMGESTVDRASLLCVLANINPHPESVPINGL
HHHHHHHHHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHHHEEEEECCCCCCCCCCCCEE
VAVEGTPLQDLPAVDPLEMVRMVATARILMPRSRVRLSAGREQLGREAQILCLQAGADSI
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCE
FYGDSLLTTSNPDVKTDRELLSQAGVHANWEESDE
EECCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA