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The map label for this gene is ilvE [H]

Identifier: 33863000

GI number: 33863000

Start: 790022

End: 790942

Strand: Direct

Name: ilvE [H]

Synonym: PMT0728

Alternate gene names: 33863000

Gene position: 790022-790942 (Clockwise)

Preceding gene: 33862998

Following gene: 33863001

Centisome position: 32.77

GC content: 48.64

Gene sequence:

>921_bases
ATGCATCAGTTCCTGCCTTATGCCTGGTTCCAGGGAGCTTGTGTTCCATTCGAAGAGGCCAAGATTTCTGTTGCCACACA
TGCTTTGCACTACGGCACAGGCGCTTTCGGAGGCATGCGTGCCATACCTGATCCCCAAAACAGCAACACCATGTTGCTCT
TTAGGGCAGACCGTCATGCCAAAAGACTCAGTCAAAGTGCTCGATTGTTGCTGACGGAACTCAGCGAGACAAGAATTCTC
TCAGCGCTTGAAGCAATGTTGCAGGCGAATCGACCAACCACTCCCATTTATCTACGACCTTTCGTCTACACGAGTGATCT
AGGCATTGCGCCTCGACTCCACAATATTGAAACTGACTTCCTTATCTATGGTCTAGAACTTGGCGACTACCTATCTCCAG
AAGGCGTGAGCTGCCGGATCAGTAGCTTTACGCGCCAAGAGGATCGCTCACTGCCGCTACGAGGCAAGATCAGTGGTGCT
TACATCACAAGCTCTCTCGCGAAAACGGAAGCGGTCTTAAGTGGATTCGGTGAAGCATTGTTGCTCAATAGCAGAGGCAA
GATCAGTGAAGCAAGTGGCATGAACCTATTCCTGGTCAGGGATGGTCAGTTGATTACCCCCGGTGTTGATCAAGACATTT
TGGAAGGGATCACGCGATCCAGTGTCCTTGAACTGGCACGGGTGATGGGCTTATCTGTGATTGAACGCCCTGTGGACAAA
ACGGAACTATTTATTGCTGATGAGGTGTTCCTTACCGGAACAGCTGCAAAAATCACGCCAATACGTCAGATTGAATCAAC
CATATTGGCTACAGAACGACCGATTATGAATGCTCTTCGAGACCGTCTAATCGAGATAACTCAAGGACGTGATAAGGATT
ATGAGCACTGGATAACACGTATTTGTATGACTAATGACTGA

Upstream 100 bases:

>100_bases
CAGGCTTGCTAAACGATGCATTGGCTCATTCTTTGATGGGAAATGCCACGTATGAGACGAGGTAATGAGAAGATTTGGCC
ATTAGGGCCCTATCCAAGTA

Downstream 100 bases:

>100_bases
TTCTTGAGAAGAGCAACCGATCTATCAGTAAGGGGCTATCTAGGATCGCATCAAAGCATGAGAGACAGGATGACAGCAGG
ACCATTGGATATCACCAGAC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MHQFLPYAWFQGACVPFEEAKISVATHALHYGTGAFGGMRAIPDPQNSNTMLLFRADRHAKRLSQSARLLLTELSETRIL
SALEAMLQANRPTTPIYLRPFVYTSDLGIAPRLHNIETDFLIYGLELGDYLSPEGVSCRISSFTRQEDRSLPLRGKISGA
YITSSLAKTEAVLSGFGEALLLNSRGKISEASGMNLFLVRDGQLITPGVDQDILEGITRSSVLELARVMGLSVIERPVDK
TELFIADEVFLTGTAAKITPIRQIESTILATERPIMNALRDRLIEITQGRDKDYEHWITRICMTND

Sequences:

>Translated_306_residues
MHQFLPYAWFQGACVPFEEAKISVATHALHYGTGAFGGMRAIPDPQNSNTMLLFRADRHAKRLSQSARLLLTELSETRIL
SALEAMLQANRPTTPIYLRPFVYTSDLGIAPRLHNIETDFLIYGLELGDYLSPEGVSCRISSFTRQEDRSLPLRGKISGA
YITSSLAKTEAVLSGFGEALLLNSRGKISEASGMNLFLVRDGQLITPGVDQDILEGITRSSVLELARVMGLSVIERPVDK
TELFIADEVFLTGTAAKITPIRQIESTILATERPIMNALRDRLIEITQGRDKDYEHWITRICMTND
>Mature_306_residues
MHQFLPYAWFQGACVPFEEAKISVATHALHYGTGAFGGMRAIPDPQNSNTMLLFRADRHAKRLSQSARLLLTELSETRIL
SALEAMLQANRPTTPIYLRPFVYTSDLGIAPRLHNIETDFLIYGLELGDYLSPEGVSCRISSFTRQEDRSLPLRGKISGA
YITSSLAKTEAVLSGFGEALLLNSRGKISEASGMNLFLVRDGQLITPGVDQDILEGITRSSVLELARVMGLSVIERPVDK
TELFIADEVFLTGTAAKITPIRQIESTILATERPIMNALRDRLIEITQGRDKDYEHWITRICMTND

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=304, Percent_Identity=32.8947368421053, Blast_Score=180, Evalue=9e-47,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34032; Mature: 34032

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQFLPYAWFQGACVPFEEAKISVATHALHYGTGAFGGMRAIPDPQNSNTMLLFRADRHA
CCCCCCCHHHCCCCCCHHHCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHH
KRLSQSARLLLTELSETRILSALEAMLQANRPTTPIYLRPFVYTSDLGIAPRLHNIETDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCE
LIYGLELGDYLSPEGVSCRISSFTRQEDRSLPLRGKISGAYITSSLAKTEAVLSGFGEAL
EEEEEECCCCCCCCCCEEEEHHHHCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LLNSRGKISEASGMNLFLVRDGQLITPGVDQDILEGITRSSVLELARVMGLSVIERPVDK
EECCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TELFIADEVFLTGTAAKITPIRQIESTILATERPIMNALRDRLIEITQGRDKDYEHWITR
CEEEEEEEEEEECCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ICMTND
HHCCCC
>Mature Secondary Structure
MHQFLPYAWFQGACVPFEEAKISVATHALHYGTGAFGGMRAIPDPQNSNTMLLFRADRHA
CCCCCCCHHHCCCCCCHHHCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCHHH
KRLSQSARLLLTELSETRILSALEAMLQANRPTTPIYLRPFVYTSDLGIAPRLHNIETDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCE
LIYGLELGDYLSPEGVSCRISSFTRQEDRSLPLRGKISGAYITSSLAKTEAVLSGFGEAL
EEEEEECCCCCCCCCCEEEEHHHHCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
LLNSRGKISEASGMNLFLVRDGQLITPGVDQDILEGITRSSVLELARVMGLSVIERPVDK
EECCCCCCCCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TELFIADEVFLTGTAAKITPIRQIESTILATERPIMNALRDRLIEITQGRDKDYEHWITR
CEEEEEEEEEEECCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ICMTND
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]