Click here to switch to the map view.

The map label for this gene is lacF [H]

Identifier: 33862940

GI number: 33862940

Start: 724886

End: 725794

Strand: Direct

Name: lacF [H]

Synonym: PMT0668

Alternate gene names: 33862940

Gene position: 724886-725794 (Clockwise)

Preceding gene: 33862938

Following gene: 33862941

Centisome position: 30.07

GC content: 51.27

Gene sequence:

>909_bases
ATGGCTGGTCCTCTGTTTCGGGGATTAAGACAGTCTTGGCGGACCACTCTTGTGGCATGGGTATTCCTCTTGCCTGCGCT
CGTCCTACTCAGCTTGTCGGTGCTGATACCAGCGCTGATGGCTCTTTTGATGAGCTTCACCAAAACAGGTCTCGACGTTA
CAGAGCCCCTTGTCTTTGTGGGTTTGGCCAACCTGCGACGTCTTGCTGGTGATCCGATGTTTTTTAAGGTGCTGATCAAT
ACTTTGATCTACCTGGTTGGGGTGGTTCCACCGATTGTTTTGGGTTCTCTTGCATTGGCGGTGTTGTTGAACCGTTCCTT
ACCAGGTATTCATTTTTTACGCGGTGCGTTCTATACACCCGTGCTCGTGTCGATTGTGGTTGCGGCCATCGCATTCCGTT
GGCTTTATGCAGAGAACGGTCTTATCAATGGCTGGCTTTCCGCCTTTGTTGGCACAGATTTCGTGCCGATTGGCTTTCTC
ACGAATCCATTCTTGGCACTCCCGGCAGTGATGCTTGTAACCCTGTGGAAGGGGCTTGGTTATTACATGGTGATATTTCT
TGCTGGCCTCCAAGGAATTCCTAAAGAGCTTTATGAGGCAGCCGAACTTGATGGTAGTGATGGCTTGCGCAAACACATCG
ACATCACACTGCCGTTGTTGCGGCCTTACGTAACCTTGGTTGCGGTGGTTTCATCGATTGCGGCTACAAAAGTGTTCGAA
GAAGTGTTCTTGATGACTCAGGGGGGACCCGCCGACACCACTCGAACCCTTGTCTACTACGTTTACGACCAGGCTTTTGC
TGAGCTTGAGATCAGTTATGCCTGCACGGTGGGCTTGGCGCTTTTCCTTTTGGTCCTGTTGCTCACTGCTGTGCGTTTGG
CCTTTGCTGGTGATCAGGGCTTGCTCTGA

Upstream 100 bases:

>100_bases
TCACGAAAGCGGACATGAAAGAGCAAGTCTGCTGATCTCAGTTTGCCGGGTGGCATCAAACGCTCCCACGTAATCTCCTG
TCAGTACAGGCATTGTGGTG

Downstream 100 bases:

>100_bases
AATCTCAATTTTGTTGGGATGCAATGCTTGCAGGATTGCGTGGTTGATTCTCTCTGCATGAGCAGCGTTAAAAGCCCAAT
GATTGGGGTCGTCGGTGGTG

Product: lactose transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MAGPLFRGLRQSWRTTLVAWVFLLPALVLLSLSVLIPALMALLMSFTKTGLDVTEPLVFVGLANLRRLAGDPMFFKVLIN
TLIYLVGVVPPIVLGSLALAVLLNRSLPGIHFLRGAFYTPVLVSIVVAAIAFRWLYAENGLINGWLSAFVGTDFVPIGFL
TNPFLALPAVMLVTLWKGLGYYMVIFLAGLQGIPKELYEAAELDGSDGLRKHIDITLPLLRPYVTLVAVVSSIAATKVFE
EVFLMTQGGPADTTRTLVYYVYDQAFAELEISYACTVGLALFLLVLLLTAVRLAFAGDQGLL

Sequences:

>Translated_302_residues
MAGPLFRGLRQSWRTTLVAWVFLLPALVLLSLSVLIPALMALLMSFTKTGLDVTEPLVFVGLANLRRLAGDPMFFKVLIN
TLIYLVGVVPPIVLGSLALAVLLNRSLPGIHFLRGAFYTPVLVSIVVAAIAFRWLYAENGLINGWLSAFVGTDFVPIGFL
TNPFLALPAVMLVTLWKGLGYYMVIFLAGLQGIPKELYEAAELDGSDGLRKHIDITLPLLRPYVTLVAVVSSIAATKVFE
EVFLMTQGGPADTTRTLVYYVYDQAFAELEISYACTVGLALFLLVLLLTAVRLAFAGDQGLL
>Mature_301_residues
AGPLFRGLRQSWRTTLVAWVFLLPALVLLSLSVLIPALMALLMSFTKTGLDVTEPLVFVGLANLRRLAGDPMFFKVLINT
LIYLVGVVPPIVLGSLALAVLLNRSLPGIHFLRGAFYTPVLVSIVVAAIAFRWLYAENGLINGWLSAFVGTDFVPIGFLT
NPFLALPAVMLVTLWKGLGYYMVIFLAGLQGIPKELYEAAELDGSDGLRKHIDITLPLLRPYVTLVAVVSSIAATKVFEE
VFLMTQGGPADTTRTLVYYVYDQAFAELEISYACTVGLALFLLVLLLTAVRLAFAGDQGLL

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787570, Length=277, Percent_Identity=30.3249097472924, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789861, Length=298, Percent_Identity=28.8590604026846, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790465, Length=260, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32854; Mature: 32723

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGPLFRGLRQSWRTTLVAWVFLLPALVLLSLSVLIPALMALLMSFTKTGLDVTEPLVFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GLANLRRLAGDPMFFKVLINTLIYLVGVVPPIVLGSLALAVLLNRSLPGIHFLRGAFYTP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VLVSIVVAAIAFRWLYAENGLINGWLSAFVGTDFVPIGFLTNPFLALPAVMLVTLWKGLG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEHHHHHCHHHHHHHHHHHHHHHHHH
YYMVIFLAGLQGIPKELYEAAELDGSDGLRKHIDITLPLLRPYVTLVAVVSSIAATKVFE
HHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVFLMTQGGPADTTRTLVYYVYDQAFAELEISYACTVGLALFLLVLLLTAVRLAFAGDQG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LL
CC
>Mature Secondary Structure 
AGPLFRGLRQSWRTTLVAWVFLLPALVLLSLSVLIPALMALLMSFTKTGLDVTEPLVFV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH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CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]