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The map label for this gene is rlmH [H]

Identifier: 33862905

GI number: 33862905

Start: 686310

End: 686744

Strand: Direct

Name: rlmH [H]

Synonym: PMT0632

Alternate gene names: 33862905

Gene position: 686310-686744 (Clockwise)

Preceding gene: 33862902

Following gene: 33862906

Centisome position: 28.47

GC content: 50.8

Gene sequence:

>435_bases
TTGAGAAACTGTGCGTTTAGAATCATCGCCGTTGGCAAGGTACGCAAAGGCTGGATCCAAGACGGCCTAGCGATGTATCA
AAAACGCCTACCAGGCTTGATTATCACAGAGGTTCGTGATGCATCTATGCCGCGAGAAGCGGAAGCGATCAGGGCCGCAC
TCAATAGCAACGAAGTGCTGGTTCCTCTTAGCGAAGAAGGGGAAGCGCTGACTTCAGTGTCCTTTGCTAAACGCCTTGAA
AAATATGGCTCTCAACGTCTTGCCTTTGTGATTGGTGGCGCTGATGGCCTATCAGCAGAGCTGAAAAACTCAACCCAATG
GCAGCTCAGTTTGTCTGCGATGACGCTTCCTCATGAACTTGCTCGCTTGTTGCTTGTGGAGCAGCTCTACCGAGCACAGA
CCATCACGCAAGGAGGTAAATACCACAGGAGCTAA

Upstream 100 bases:

>100_bases
GATAGCGCCTGAAGCACTTCAAGTAACATTCTCTTCTGGGGATCTGTTGAACAGTGCTTATAACCGAGCATCATGGAGAG
TGATGTTGCTGCAACGGTTC

Downstream 100 bases:

>100_bases
CGATTCTGCAGATCTGAATTGCGATCACTGATAAAACAGCTGCATTCGATTAATGCGATAACTCTTGAGCACACTGAAAA
GGAAATCAGTTTAAGATAAT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLIITEVRDASMPREAEAIRAALNSNEVLVPLSEEGEALTSVSFAKRLE
KYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHELARLLLVEQLYRAQTITQGGKYHRS

Sequences:

>Translated_144_residues
MRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLIITEVRDASMPREAEAIRAALNSNEVLVPLSEEGEALTSVSFAKRLE
KYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHELARLLLVEQLYRAQTITQGGKYHRS
>Mature_144_residues
MRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLIITEVRDASMPREAEAIRAALNSNEVLVPLSEEGEALTSVSFAKRLE
KYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHELARLLLVEQLYRAQTITQGGKYHRS

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=153, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 15972; Mature: 15972

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLIITEVRDASMPREAEAIRAALNSNEVL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEE
VPLSEEGEALTSVSFAKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHEL
EEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCCEEEEEEHHHHCHHHH
ARLLLVEQLYRAQTITQGGKYHRS
HHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRNCAFRIIAVGKVRKGWIQDGLAMYQKRLPGLIITEVRDASMPREAEAIRAALNSNEVL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEE
VPLSEEGEALTSVSFAKRLEKYGSQRLAFVIGGADGLSAELKNSTQWQLSLSAMTLPHEL
EEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHCCCCCEEEEEEHHHHCHHHH
ARLLLVEQLYRAQTITQGGKYHRS
HHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA