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The map label for this gene is glgB [H]

Identifier: 33862717

GI number: 33862717

Start: 500733

End: 503003

Strand: Direct

Name: glgB [H]

Synonym: PMT0444

Alternate gene names: 33862717

Gene position: 500733-503003 (Clockwise)

Preceding gene: 33862716

Following gene: 33862718

Centisome position: 20.77

GC content: 50.11

Gene sequence:

>2271_bases
ATGACCACCAGTGTTGCCCTCGACTGGGTGGTTCAAGACGGCCAAAGACTTGCTGAATGTCGCCACGATGATCCTTTCTC
GCTGTTGGGTCCTCAATCTCATGAGGGGCAATGGATCGTGCGCATCTGGATGCCGGAGGCAAGCCAAGTAGAGCTGCTTT
GTGATGGCAGAACGACTGCCATGACAACGCCCAATCACTCCTGGATCTTCGAAGCAGCTCTCAACCAAAATCCCGGCCGC
ACATACCAGGTGCGGGTTAAACGCGCAGGAATCGTGCATGAGCAGCACGACCCCTGGGCCTTCCACGATGAATGGATGGG
GGAGATGGACCGTCACCTCTTTGCGGAGGGCAACCATCACCACATTTGGCAACGTATGGGCGCCCACCTGATGGAGCGTG
AGGGTGTTGAGGGAGTGATGTTCTGCCTCTGGGCACCCCATGCATGCAGTGTGGCTGTTCTCGGAGAATTAAACGGATGG
GATGGCCGTCATCATCCGATGCAGCGTCGCCAGGGAGGCCTTTGGGAGCTGTTTATTCCTGGGTTTAAAGAAGGGACCCT
TTATAAATACGAAATCCGAACGCAGGAAGGCCATTGCTATCAGAAAGCTGATCCCTATGGTTTCCAGCACGAGGTGCGCC
CAGCGACAAGCTCCGTAGTCGCGAGGCTTGATCGCTATCAATGGCAGGACGAGCAATGGATGCGGCAACGGGATAGTCGT
AATGCTCTCGATCAACCAATTTCTGTCTATGAAATGCATTTAGGCAGCTGGATTCATGCAGCAACAGATGAACCGTATAT
CGAGTTGGATGGCACGCCCCGGGCGCCGGTACCCGCTGCGGACATGAAGCCTGGAGCTCGTCTCCTGACCTATCCCGAAC
TAGCCGACCAGTTAATCCCCTACGTGAAAGATCGGGGATTCACCCACATTGAACTGATGCCAATCTCAGAGCATCCATTT
GATGGCTCATGGGGATACCAGGTCACAGGGTGGTATGCACCCACCAGCCGATTTGGAAGTCCGGATGAATTCCGTGCATT
TGTTGATCGCTGCCACGCCGAAGGTTTAGGTGTGATCATCGATTGGGTGCCAGGCCATTTCCCTAAGGATGGTCATGGCC
TGGCCTTCTTTGATGGAACCCATCTCTACGAACATTCTGATCCACGCATTGGTGAACACAAGGAATGGGGCACGTTGATC
TTCAACTACAGCCGCAACGAGGTTCGCAACTTTCTCGTTGCCAATCTTGTGTATTGGTTTGAGCAATTCCATATTGATGG
TATTCGTGTTGATGCGGTGGCTTCGATGCTTTATCGCGACTATCTTCGACCTGATGGAGAATGGATTGCCAATGAAGATG
GAGGGCGAGAAAATACAGAAGCAGTTCGATTCCTCCAACAGGCCAATCACGTTCTTTTCCAGCACTTCCCTGGAGCGCTT
TCAATTGCGGAGGAGTCAACAACTTGGCCCATGGTTACTCAGCCCACTGATATTGGCGGGCTGGGATTCAATCTTAAATG
GAATATGGGTTGGATGCATGACATGCTCGATTATTTCGAGTTAGACCCTTGGTTTAGGCAGTTTCACCAGAACAACATCA
CTTTTTCGATTTGGTATGCCTACACCGAAAACTTCATGCTGGCACTTAGTCATGATGAGGTTGTGCATGGTAAAAGCAAT
CTTCTCCATAAGATGCCAGGAGATGACTGGCAGAAATTTGCAAATGTTCGTGCATTGCTTGCCTATATGTGGACCCATCC
TGGCAAGAAAACTATCTTCATGGGAATGGAATTTGGTCAACGATCGGAATGGAATGTATGGGGCGATCTGCAATGGGAGC
TGTTGAAACATGATCCTCATAAGGGTCTGCAGAGATTGGTTGATGACCTCAACACTTTCTATAAAGGCGAGCCTGCTCTA
TGGAAGGATGACTTTGATCAATATGGATTCCAATGGATTGATTGCAATGATAATCGTCATTCTGTAATCAGCTTTATGCG
CCGTGAAAGTAGTGGCGGAACTTGGCTTGTCGTCGTCGCCAACTTCACACCTCAAAGTCATTCAAATTACAGAATTGGCG
TGCCTATGAGTGGCTATTATCAGGAAGTTTTCAACACAGATAGCTCTTGTTACGGCGGTAGAAACCTCGGCAATATGGGC
GGTAAAAACACTGATGAATTCAATATCCATGGATATGAGCAGTCTCTTGAACTCTGTCTACCAGCGCTGAGCGTGCTGGT
CTTCCGTCATGACCCGAAGCGAAGTCTTTGA

Upstream 100 bases:

>100_bases
TTGATCAGCGTTCGCTGATTCAACTGCTCTCTCTGGTGCTCAGTCTGCCCTTGCGGTCCATGAATCGACCTAAGCTCCCG
GCCATTGATGTCTTTGCCCC

Downstream 100 bases:

>100_bases
GTGCAGCCAAAGCCAATGAACATGTGACGAAGCTGCGTTCTCAACATCACTGATGGCCTGAACAACAACGGCCTTCAGGT
AGGTTTTCTGACTGATCCTG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTTSVALDWVVQDGQRLAECRHDDPFSLLGPQSHEGQWIVRIWMPEASQVELLCDGRTTAMTTPNHSWIFEAALNQNPGR
TYQVRVKRAGIVHEQHDPWAFHDEWMGEMDRHLFAEGNHHHIWQRMGAHLMEREGVEGVMFCLWAPHACSVAVLGELNGW
DGRHHPMQRRQGGLWELFIPGFKEGTLYKYEIRTQEGHCYQKADPYGFQHEVRPATSSVVARLDRYQWQDEQWMRQRDSR
NALDQPISVYEMHLGSWIHAATDEPYIELDGTPRAPVPAADMKPGARLLTYPELADQLIPYVKDRGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGSPDEFRAFVDRCHAEGLGVIIDWVPGHFPKDGHGLAFFDGTHLYEHSDPRIGEHKEWGTLI
FNYSRNEVRNFLVANLVYWFEQFHIDGIRVDAVASMLYRDYLRPDGEWIANEDGGRENTEAVRFLQQANHVLFQHFPGAL
SIAEESTTWPMVTQPTDIGGLGFNLKWNMGWMHDMLDYFELDPWFRQFHQNNITFSIWYAYTENFMLALSHDEVVHGKSN
LLHKMPGDDWQKFANVRALLAYMWTHPGKKTIFMGMEFGQRSEWNVWGDLQWELLKHDPHKGLQRLVDDLNTFYKGEPAL
WKDDFDQYGFQWIDCNDNRHSVISFMRRESSGGTWLVVVANFTPQSHSNYRIGVPMSGYYQEVFNTDSSCYGGRNLGNMG
GKNTDEFNIHGYEQSLELCLPALSVLVFRHDPKRSL

Sequences:

>Translated_756_residues
MTTSVALDWVVQDGQRLAECRHDDPFSLLGPQSHEGQWIVRIWMPEASQVELLCDGRTTAMTTPNHSWIFEAALNQNPGR
TYQVRVKRAGIVHEQHDPWAFHDEWMGEMDRHLFAEGNHHHIWQRMGAHLMEREGVEGVMFCLWAPHACSVAVLGELNGW
DGRHHPMQRRQGGLWELFIPGFKEGTLYKYEIRTQEGHCYQKADPYGFQHEVRPATSSVVARLDRYQWQDEQWMRQRDSR
NALDQPISVYEMHLGSWIHAATDEPYIELDGTPRAPVPAADMKPGARLLTYPELADQLIPYVKDRGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRFGSPDEFRAFVDRCHAEGLGVIIDWVPGHFPKDGHGLAFFDGTHLYEHSDPRIGEHKEWGTLI
FNYSRNEVRNFLVANLVYWFEQFHIDGIRVDAVASMLYRDYLRPDGEWIANEDGGRENTEAVRFLQQANHVLFQHFPGAL
SIAEESTTWPMVTQPTDIGGLGFNLKWNMGWMHDMLDYFELDPWFRQFHQNNITFSIWYAYTENFMLALSHDEVVHGKSN
LLHKMPGDDWQKFANVRALLAYMWTHPGKKTIFMGMEFGQRSEWNVWGDLQWELLKHDPHKGLQRLVDDLNTFYKGEPAL
WKDDFDQYGFQWIDCNDNRHSVISFMRRESSGGTWLVVVANFTPQSHSNYRIGVPMSGYYQEVFNTDSSCYGGRNLGNMG
GKNTDEFNIHGYEQSLELCLPALSVLVFRHDPKRSL
>Mature_755_residues
TTSVALDWVVQDGQRLAECRHDDPFSLLGPQSHEGQWIVRIWMPEASQVELLCDGRTTAMTTPNHSWIFEAALNQNPGRT
YQVRVKRAGIVHEQHDPWAFHDEWMGEMDRHLFAEGNHHHIWQRMGAHLMEREGVEGVMFCLWAPHACSVAVLGELNGWD
GRHHPMQRRQGGLWELFIPGFKEGTLYKYEIRTQEGHCYQKADPYGFQHEVRPATSSVVARLDRYQWQDEQWMRQRDSRN
ALDQPISVYEMHLGSWIHAATDEPYIELDGTPRAPVPAADMKPGARLLTYPELADQLIPYVKDRGFTHIELMPISEHPFD
GSWGYQVTGWYAPTSRFGSPDEFRAFVDRCHAEGLGVIIDWVPGHFPKDGHGLAFFDGTHLYEHSDPRIGEHKEWGTLIF
NYSRNEVRNFLVANLVYWFEQFHIDGIRVDAVASMLYRDYLRPDGEWIANEDGGRENTEAVRFLQQANHVLFQHFPGALS
IAEESTTWPMVTQPTDIGGLGFNLKWNMGWMHDMLDYFELDPWFRQFHQNNITFSIWYAYTENFMLALSHDEVVHGKSNL
LHKMPGDDWQKFANVRALLAYMWTHPGKKTIFMGMEFGQRSEWNVWGDLQWELLKHDPHKGLQRLVDDLNTFYKGEPALW
KDDFDQYGFQWIDCNDNRHSVISFMRRESSGGTWLVVVANFTPQSHSNYRIGVPMSGYYQEVFNTDSSCYGGRNLGNMGG
KNTDEFNIHGYEQSLELCLPALSVLVFRHDPKRSL

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=668, Percent_Identity=26.3473053892216, Blast_Score=194, Evalue=3e-49,
Organism=Escherichia coli, GI1789839, Length=732, Percent_Identity=49.4535519125683, Blast_Score=681, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=674, Percent_Identity=24.3323442136499, Blast_Score=166, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32564391, Length=644, Percent_Identity=24.0683229813665, Blast_Score=149, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=510, Percent_Identity=27.843137254902, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI28573410, Length=671, Percent_Identity=27.2727272727273, Blast_Score=208, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 87746; Mature: 87615

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSVALDWVVQDGQRLAECRHDDPFSLLGPQSHEGQWIVRIWMPEASQVELLCDGRTTA
CCCCEEEHEEHHCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCEEE
MTTPNHSWIFEAALNQNPGRTYQVRVKRAGIVHEQHDPWAFHDEWMGEMDRHLFAEGNHH
EECCCCCEEEEEECCCCCCCEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCH
HIWQRMGAHLMEREGVEGVMFCLWAPHACSVAVLGELNGWDGRHHPMQRRQGGLWELFIP
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHCCCEEEEECC
GFKEGTLYKYEIRTQEGHCYQKADPYGFQHEVRPATSSVVARLDRYQWQDEQWMRQRDSR
CCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
NALDQPISVYEMHLGSWIHAATDEPYIELDGTPRAPVPAADMKPGARLLTYPELADQLIP
HHHCCCHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
YVKDRGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGSPDEFRAFVDRCHAEGLGVII
HHHCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEE
DWVPGHFPKDGHGLAFFDGTHLYEHSDPRIGEHKEWGTLIFNYSRNEVRNFLVANLVYWF
ECCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EQFHIDGIRVDAVASMLYRDYLRPDGEWIANEDGGRENTEAVRFLQQANHVLFQHFPGAL
HHHCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWPMVTQPTDIGGLGFNLKWNMGWMHDMLDYFELDPWFRQFHQNNITFSIWYA
EECCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
YTENFMLALSHDEVVHGKSNLLHKMPGDDWQKFANVRALLAYMWTHPGKKTIFMGMEFGQ
ECCCEEEEEECCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
RSEWNVWGDLQWELLKHDPHKGLQRLVDDLNTFYKGEPALWKDDFDQYGFQWIDCNDNRH
CCCCCCCCCEEHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEECCCCHH
SVISFMRRESSGGTWLVVVANFTPQSHSNYRIGVPMSGYYQEVFNTDSSCYGGRNLGNMG
HHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCC
GKNTDEFNIHGYEQSLELCLPALSVLVFRHDPKRSL
CCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTSVALDWVVQDGQRLAECRHDDPFSLLGPQSHEGQWIVRIWMPEASQVELLCDGRTTA
CCCEEEHEEHHCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCEEE
MTTPNHSWIFEAALNQNPGRTYQVRVKRAGIVHEQHDPWAFHDEWMGEMDRHLFAEGNHH
EECCCCCEEEEEECCCCCCCEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCH
HIWQRMGAHLMEREGVEGVMFCLWAPHACSVAVLGELNGWDGRHHPMQRRQGGLWELFIP
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCHHHHCCCEEEEECC
GFKEGTLYKYEIRTQEGHCYQKADPYGFQHEVRPATSSVVARLDRYQWQDEQWMRQRDSR
CCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCC
NALDQPISVYEMHLGSWIHAATDEPYIELDGTPRAPVPAADMKPGARLLTYPELADQLIP
HHHCCCHHHHHHHHCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
YVKDRGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGSPDEFRAFVDRCHAEGLGVII
HHHCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEE
DWVPGHFPKDGHGLAFFDGTHLYEHSDPRIGEHKEWGTLIFNYSRNEVRNFLVANLVYWF
ECCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EQFHIDGIRVDAVASMLYRDYLRPDGEWIANEDGGRENTEAVRFLQQANHVLFQHFPGAL
HHHCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWPMVTQPTDIGGLGFNLKWNMGWMHDMLDYFELDPWFRQFHQNNITFSIWYA
EECCCCCCCCEEECCCCCCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEEE
YTENFMLALSHDEVVHGKSNLLHKMPGDDWQKFANVRALLAYMWTHPGKKTIFMGMEFGQ
ECCCEEEEEECCCEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
RSEWNVWGDLQWELLKHDPHKGLQRLVDDLNTFYKGEPALWKDDFDQYGFQWIDCNDNRH
CCCCCCCCCEEHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCEEEECCCCHH
SVISFMRRESSGGTWLVVVANFTPQSHSNYRIGVPMSGYYQEVFNTDSSCYGGRNLGNMG
HHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCC
GKNTDEFNIHGYEQSLELCLPALSVLVFRHDPKRSL
CCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA