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The map label for this gene is lasT

Identifier: 30065639

GI number: 30065639

Start: 4598644

End: 4599330

Strand: Direct

Name: lasT

Synonym: S4706

Alternate gene names: 30065639

Gene position: 4598644-4599330 (Clockwise)

Preceding gene: 30065638

Following gene: NA

Centisome position: 99.98

GC content: 50.22

Gene sequence:

>687_bases
ATGCGTATAACGATTATCCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGG
TTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATA
TTATTGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGT
AGTCGGGCGAAATATCATTACTACGCCACGCCAGTAGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCA
TGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTGCTTACTGGTGTGC
CGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGAAGGTCTATTGCTATCAATTAGCAACATTAATACAA
CAACCGGCGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGAC
TCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAA
TGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAA

Upstream 100 bases:

>100_bases
TTTTTCTGCGGCTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTATT
TGGTCAAGAAGAGTGGCAAT

Downstream 100 bases:

>100_bases
AAAACGCCTTAGTAAGTATTTTTCAGCTTTTCATTCTGACTGCAACGGGCAATATGTCTCTGTGTGGATTAAAAAAAGAG
TGTCTGATAGCAGCTTCTGA

Product: putative RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPATRWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTAR
SRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVKVYCYQLATLIQ
QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK

Sequences:

>Translated_228_residues
MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPATRWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTAR
SRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVKVYCYQLATLIQ
QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
>Mature_228_residues
MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPATRWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTAR
SRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVKVYCYQLATLIQ
QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=228, Percent_Identity=97.8070175438597, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1788881, Length=153, Percent_Identity=37.2549019607843, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25298; Mature: 25298

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPATRWVAHGSGDIIDNIKV
CEEEEEEEECCCCCHHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHCCCCCHHCCHHH
FPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLT
HHHHHHHHHHCCEEEEHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
NEELALADVLTGVPMVADYPSLNLGQAVKVYCYQLATLIQQPAKSDTTADQHQLQALRER
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPATRWVAHGSGDIIDNIKV
CEEEEEEEECCCCCHHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHCCCCCHHCCHHH
FPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSWMSHAALVFGREDSGLT
HHHHHHHHHHCCEEEEHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
NEELALADVLTGVPMVADYPSLNLGQAVKVYCYQLATLIQQPAKSDTTADQHQLQALRER
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
VMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLHDIEKNITK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8022271 [H]