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The map label for this gene is yhiW

Identifier: 30065158

GI number: 30065158

Start: 4076000

End: 4076728

Strand: Reverse

Name: yhiW

Synonym: S4171

Alternate gene names: 30065158

Gene position: 4076728-4076000 (Counterclockwise)

Preceding gene: 30065159

Following gene: 30065156

Centisome position: 88.64

GC content: 42.11

Gene sequence:

>729_bases
ATGACTCATGTCTGCTCGGTGATCCTCATTCGTCGTTCATTCGATATTTATCATGAACAGCAAAAAATATCGCTGCATAA
CGAGAGTATCCTGCTGCTGGAGAAAAATTTGGCAGACGATTTTGCGTTTTGTTCACCGGATACGCGACGACTGGATATCG
ATGAGCTGACAGTTTGCCATTACTTACAAAATATTCGTCAGCTACCACGCAATTTAGGGTTACATAGCAAAGACCGTCTG
TTAATTAACCAGTCACCCCCCATGCCGCTGGTGACGGCGATTTTTGATAGCTTCAATGAATCCGGGGTAAATTCACCGAT
ACTGAGCAATATGCTCTACCTTTCCTGTTTATCGATGTTTTCTCATAAGAAAGAACTGATCCCCTTACTTTTCAATAGCA
TCAGCACTGTTTCAGGAAAAGTTGAACGCCTTATTAGCTTTGATATCGCCAAACGTTGGTATCTGCGCGATATCGCGGAA
AGAATGTATACCAGCGAGAGTCTCATCAAAAAAAAGTTGCAGGATGAAAATACCTGTTTCAGTAAAATATTACTCGCTTC
CAGGATGTCGATGGCCAGACGATTACTCGAGTTACGTCAAATTCCTCTGCATACTATTGCGGAAAAATGTGGCTATAGCA
GTACGTCGTACTTTATAAACACATTTCGACAATATTATGGTGTAACGCCACATCAGTTTGCGCAACATTCGCCAGGTACC
TTTTCCTGA

Upstream 100 bases:

>100_bases
CTACCAAATCTGGCAGTTTTTGCGCTAAGAAACAGTCTGTCATCATTTCATTAGTATACTGAAATTGAAATAATCGCAGT
ATGAAATATAAGGGATAATC

Downstream 100 bases:

>100_bases
CATATTTTGCATTTGAATATTGGTCAGGATCTCACACCTGCTTCATGTGAAACTCTTCCCTGATGATTTCTGCCGGGCTA
CCGGCTAGTTCTCTTTCGCA

Product: putative ARAC-type regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRL
LINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS

Sequences:

>Translated_242_residues
MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRL
LINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
FS
>Mature_241_residues
THVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLL
INQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAER
MYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGTF
S

Specific function: Depending on the conditions (growth phase and medium), acts as a positive or negative regulator of gadA and gadBC. Repression occurs directly or via the repression of the expression of gadX. Activation occurs directly by the binding of gadW to the gadA an

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789932, Length=242, Percent_Identity=100, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1786776, Length=241, Percent_Identity=35.6846473029046, Blast_Score=167, Evalue=8e-43,
Organism=Escherichia coli, GI1787776, Length=237, Percent_Identity=34.1772151898734, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1789933, Length=209, Percent_Identity=32.5358851674641, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790557, Length=133, Percent_Identity=37.593984962406, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786778, Length=124, Percent_Identity=41.1290322580645, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): GADW_ECO57 (P63202)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91178
- PIR:   D86024
- RefSeq:   NP_290095.1
- RefSeq:   NP_312422.1
- ProteinModelPortal:   P63202
- SMR:   P63202
- EnsemblBacteria:   EBESCT00000025964
- EnsemblBacteria:   EBESCT00000059915
- GeneID:   915748
- GeneID:   961148
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4928
- KEGG:   ecs:ECs4395
- GeneTree:   EBGT00050000008641
- HOGENOM:   HBG467631
- OMA:   INQLPPM
- ProtClustDB:   CLSK880699
- BioCyc:   ECOL83334:ECS4395-MONOMER
- GO:   GO:0005622
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- Gene3D:   G3DSA:1.10.10.60
- PRINTS:   PR00032
- SMART:   SM00342

Pfam domain/function: PF00165 HTH_AraC; SSF46689 Homeodomain_like

EC number: NA

Molecular weight: Translated: 28029; Mature: 27898

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00041 HTH_ARAC_FAMILY_1; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCH
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC
>Mature Secondary Structure 
THVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFCSPDTRRLDIDELTVCH
CHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHH
YLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESGVNSPILSNMLYLSCLSMF
HHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTSESLIKKKLQDENTCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFRQYYGVTPHQFAQHSPGT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHCCCCCC
FS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796