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The map label for this gene is insF1 [C]

Identifier: 30065021

GI number: 30065021

Start: 3916068

End: 3916781

Strand: Reverse

Name: insF1 [C]

Synonym: S4021

Alternate gene names: 30065021

Gene position: 3916781-3916068 (Counterclockwise)

Preceding gene: 30065022

Following gene: 30065016

Centisome position: 85.16

GC content: 52.24

Gene sequence:

>714_bases
GTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGCGGCTCCAGACGGAGCTGGCAGAGAATGGCATCATCGTTGG
TCGTGACCGACTGGCACGTCTTCGTAAGGAGCTGAGGCTACGCTGTAAGCAGAAACGCAAGTTCAGAGCGACTACGAACC
CGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTACAGCACCAAATCAGGTCTGGGTGGCG
GACCTGACGTATGTTGCCACACAGGAGGGATGGTTGTACCTCGCTGGCATCAAAGATGTTTATACGTGCGAAATTGTCGG
CTACGCCATGGGAGAGCGCATGACAAAAGAGCTGACAGGTAAAGCCCTGTTTATGGCGCTCAGGAGCCAGCGCCCACCTG
CCGGGCTAATCCACCACTCTGATCGAGGTTCACAGTACTGCGCATACGATTACCGGGTCATACAGGAGCAGTTTGGTCTG
AAAACATCAATGTCGCGTAAAGGTAACTGTTACGACAACGCTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAGAG
CCTGAGCCACTATCGTTTTAATAACCGGGATGAAGCCATCTCAGTAATACGGGAATACATTGAGATTTTCTACAATCGTC
AGCGTCGTCACTCTCGTCTGGGGAATATCTCCCCGGCAGCCTTCAGGGAAAAATATCATCAGATGGCTGCTTAA

Upstream 100 bases:

>100_bases
TCAGGTATTTGGTGTATCCAGGAGCGGTTATTACAACTGGGTACAGCATGAACCCTCAGACAGAAAACAAAGTGATGAGC
GGCTAAAACCGGAGATTAAG

Downstream 100 bases:

>100_bases
AAAAAGAACAAATGGTAGTGTCCGCTATTGCCAGTACACCTCACCCAGGTGTCGCTCTGTTCCCAGCAAATGCTGATATG
CGGATAACGTAAAAAGGTGT

Product: IS600 orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGL
KTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Sequences:

>Translated_237_residues
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGL
KTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
>Mature_236_residues
AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVAD
LTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLK
TSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=194, Percent_Identity=34.020618556701, Blast_Score=121, Evalue=5e-28,
Organism=Escherichia coli, GI1788405, Length=230, Percent_Identity=42.1739130434783, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1787263, Length=230, Percent_Identity=42.1739130434783, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786753, Length=230, Percent_Identity=42.1739130434783, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786570, Length=230, Percent_Identity=42.1739130434783, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1786490, Length=230, Percent_Identity=42.1739130434783, Blast_Score=185, Evalue=3e-48,
Organism=Escherichia coli, GI1789981, Length=229, Percent_Identity=34.4978165938865, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 27540; Mature: 27409

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPV
CCCCCCCHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCC
APNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTG
CCHHHHCCCCCCCCCEEEEEHEEEEEECCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHH
KALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFW
HHHHHHHHCCCCCCCEEEECCCCCCEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHH
GTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPV
CCCCCCHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCC
APNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTG
CCHHHHCCCCCCCCCEEEEEHEEEEEECCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHH
KALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFW
HHHHHHHHCCCCCCCEEEECCCCCCEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHH
GTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]