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The map label for this gene is corA

Identifier: 30064887

GI number: 30064887

Start: 3757791

End: 3758741

Strand: Reverse

Name: corA

Synonym: S3861

Alternate gene names: 30064887

Gene position: 3758741-3757791 (Counterclockwise)

Preceding gene: 30064889

Following gene: 30064882

Centisome position: 81.72

GC content: 52.68

Gene sequence:

>951_bases
ATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGT
ATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCC
CGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTT
GAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCG
TGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGC
TGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGG
GTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACGCTGGCGGAACTGGAAGATATCGGCTGGAA
AGTTCGCCTGTGTCTGATGGATACCCAGCGCGCTCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGG
AGCAGGCGCGTGAAATCCTGCGCGATATCGAATCCCTGCTGCCGCATAACGAATCCCTGTTCCAGAAGGTGAACTTCCTG
ATGCAGGCGGCAATGGGTTTTATCAACATCGAGCAGAACCGCATCATCAAAATTTTCTCGGTGGTATCCGTCGTGTTCCT
GCCGCCAACGCTGGTTGCTTCCAGCTACGGGATGAACTTTGAGTTTATGCCAGAACTGAAGTGGAGTTTCGGCTACCCTG
GCGCGATTATCTTTATGATCCTCGCGGGCCTGGCACCGTATCTGTACTTTAAGCGGAAGAACTGGTTGTAA

Upstream 100 bases:

>100_bases
TCCCGTCGCGCTGAGTCAGGCTGTTTAATGGTCTGAAACCCAATTTGTTTCTGTGTGCCCACCGAACTGTCCGATATTTT
AAGCATTGGGAGTCCCGGTC

Downstream 100 bases:

>100_bases
AAAGAAGGCGGTGGCTTAGCCTGGCTAAGCCACCTGTTATTCAAAGGCTCCAGGTATTTAACCCTTTTACCTCTTTCTCA
TAAAACCATTTGTTCGTGTT

Product: magnesium/nickel/cobalt transporter CorA

Products: Ni2; [Cytoplasm]; Co; 2 [Cytoplasm]; Mg (II) [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQSELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFF
EDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFL
MQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL

Sequences:

>Translated_316_residues
MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQSELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFF
EDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFL
MQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL
>Mature_316_residues
MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQSELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFF
EDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFL
MQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL

Specific function: Mediates influx of magnesium ions

COG id: COG0598

COG function: function code P; Mg2+ and Co2+ transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family

Homologues:

Organism=Escherichia coli, GI2367297, Length=316, Percent_Identity=100, Blast_Score=642, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CORA_ECO57 (P0ABI6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B91222
- PIR:   E86068
- RefSeq:   NP_290445.1
- RefSeq:   NP_312773.1
- ProteinModelPortal:   P0ABI6
- EnsemblBacteria:   EBESCT00000025072
- EnsemblBacteria:   EBESCT00000057760
- GeneID:   915181
- GeneID:   960346
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5333
- KEGG:   ecs:ECs4746
- GeneTree:   EBGT00050000011244
- HOGENOM:   HBG350378
- OMA:   TQGFINI
- ProtClustDB:   PRK11085
- BioCyc:   ECOL83334:ECS4746-MONOMER
- InterPro:   IPR004488
- InterPro:   IPR002523
- PANTHER:   PTHR21535:SF1
- TIGRFAMs:   TIGR00383

Pfam domain/function: PF01544 CorA

EC number: NA

Molecular weight: Translated: 36590; Mature: 36590

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15c1c69c)-; HASH(0x15dcd73c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQSELGQSLATRPELEDIE
CCCCEEECCCCEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
ASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRAR
HHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHH
SQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSRVIMEGHQGDEYDEALSTLAE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFL
HHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMI
HHHHHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHH
LAGLAPYLYFKRKNWL
HHHHHHHHHHHCCCCH
>Mature Secondary Structure
MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQSELGQSLATRPELEDIE
CCCCEEECCCCEEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHH
ASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLFTLRERELPAFRLYRMRAR
HHHHHCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHH
SQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSRVIMEGHQGDEYDEALSTLAE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQAREILRDIESLLPHNESLFQKVNFL
HHHCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSYGMNFEFMPELKWSFGYPGAIIFMI
HHHHHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHH
LAGLAPYLYFKRKNWL
HHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; Co; 2 [Periplasm]; Mg (II) [Periplasm] [C]

Specific reaction: Ni2+ [Periplasm] = Ni2+ [Cytoplasm] Co+2 [Periplasm] = Co+2 [Cytoplasm] Mg (II) [Periplasm] = Mg (II) [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796