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The map label for this gene is frwD

Identifier: 30064760

GI number: 30064760

Start: 3610672

End: 3611013

Strand: Reverse

Name: frwD

Synonym: S3716

Alternate gene names: 30064760

Gene position: 3611013-3610672 (Counterclockwise)

Preceding gene: 30064761

Following gene: 30064755

Centisome position: 78.51

GC content: 56.43

Gene sequence:

>342_bases
ATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACTTATATGGCGGCGGAACGGCTGGAAAAGTT
GTGCCAGTTAGAGAAGTGGGGCGTCAGCATTGAAACTCAGGGCGCGCTGGGAACGGAGAATCGTTTAGCGGACGAGGATA
TCCGTCGGGCGGATGTTGCTCTGTTGATTACGGATATCGAGCTTGCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTG
CAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCA
AACCCATCTTATTCTGGAGTAG

Upstream 100 bases:

>100_bases
TGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCCGATGTGACAACGATGCGCGAAATGGCAGAACGGGCCGGATTTCA
GGTTACCGTGGGAGGTTAAA

Downstream 100 bases:

>100_bases
TCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGTGTTCGGTGTTTTTACGAAACAGACGG
CAGAAGTAGTTGCTGTCGAC

Product: putative fructose-like phosphotransferase EIIB subunit 3

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 3 [H]

Number of amino acids: Translated: 113; Mature: 112

Protein sequence:

>113_residues
MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV
QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE

Sequences:

>Translated_113_residues
MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYV
QCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE
>Mature_112_residues
AYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVALLITDIELAGAERFEHCRYVQ
CSIYAFLREPQRVMSAVRKVLSAPQQTHLILE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790390, Length=113, Percent_Identity=99.1150442477876, Blast_Score=228, Evalue=5e-62,
Organism=Escherichia coli, GI1790387, Length=98, Percent_Identity=43.8775510204082, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI87082348, Length=70, Percent_Identity=45.7142857142857, Blast_Score=66, Evalue=5e-13,
Organism=Escherichia coli, GI1788492, Length=65, Percent_Identity=44.6153846153846, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1788730, Length=104, Percent_Identity=31.7307692307692, Blast_Score=60, Evalue=4e-11,
Organism=Escherichia coli, GI1786951, Length=71, Percent_Identity=42.2535211267606, Blast_Score=59, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 12652; Mature: 12521

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHH
LLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE
HEEEHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHCEECC
>Mature Secondary Structure 
AYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALGTENRLADEDIRRADVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHH
LLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVLSAPQQTHLILE
HEEEHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]