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The map label for this gene is hydG

Identifier: 30064714

GI number: 30064714

Start: 3549614

End: 3550939

Strand: Reverse

Name: hydG

Synonym: S3659

Alternate gene names: 30064714

Gene position: 3550939-3549614 (Counterclockwise)

Preceding gene: 30064715

Following gene: 30064710

Centisome position: 77.21

GC content: 56.79

Gene sequence:

>1326_bases
ATGACGCACGATAATATCGATATTCTGGTGGTGGATGATGACATTAGCCACTGCACTATTTTGCAGGCTTTACTGCGCGG
CTGGGGCTATAACGTCGCGCTGGCGAACAGCGGGCGACAGGCGCTGGAGCAGGTGCGGGAACAGGTTTTTGATCTTGTGC
TTTGCGATGTGCGAATGGCGGAGATGGACGGCATCGCCACGCTGAAAGAGATCAAAGCGTTAAACCCGGCAATTCCGGTG
CTGATTATGACTGCGTACTCCAGCGTCGAGACGGCGGTAGAGGCACTGAAAACTGGGGCGCAGGATTATCTCATCAAGCC
GCTGGATTTCGATAACCTACAGGCGACGCTGGAAAAGGCGCTCGCGCATACGCACAGTATTGATGCTGAAACACCTGCGG
TGACTGCCAGCCAGTTCGGTATGGTCGGTAAAAGCCCGGCGATGCAACACCTGCTCAGTGAAATCGCCCTCGTCGCGCCA
TCGGAAGCCACGGTGCTGATCCACGGCGATTCCGGCACCGGTAAAGAGCTGGTCGCCAGGGCGATTCACGCCAGTAGCGC
ACGTAGCGAAAAACCGCTGGTAACGCTCAACTGTGCGGCACTCAACGAATCCTTGCTGGAATCTGAATTGTTCGGTCACG
AAAAAGGGGCGTTTACTGGAGCCGATAAACGCCGGGAAGGGCGCTTTGTTGAGGCGGACGGCGGCACGCTGTTTCTCGAT
GAAATTGGCGATATCTCGCCGATGATGCAGGTGCGTCTGCTGCGTGCGATTCAGGAGCGCGAAGTTCAGCGTGTCGGTAG
CAACCAGACTATCTCGGTTGATGTCCGGCTGATTGCGGCGACCCATCGCGATCTTGCCGCAGAGGTGAATGCCGGGCGTT
TTCGCCAGGATCTCTACTATCGCCTGAATGTGGTGGCGATTGAAGTACCATCGCTGCGGCAACGGCGGGAAGATATCCCT
CTGCTGGCTGGCCATTTTCTTCAGCGCTTTGCCGAGCGTAATCGCAAGGCGGTAAAAGGCTTTACGCCACAGGCGATGGA
TTTGCTGATCCACTACGACTGGCCGGGAAATATTCGTGAACTGGAAAACGCGGTGGAACGGGCGGTGGTGCTGCTGACCG
GGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGGCAAAGTCAGGATATTCAG
CCGCTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCA
GTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAG

Upstream 100 bases:

>100_bases
AACAACACGGTGGTACAATTCAGGTCGCAAGCCAGGAGGGAAAAGGCTCAACGTTCACCCTCTGGCTTCCGGTCAATATT
ACGCGTAAGGACCCACAAGG

Downstream 100 bases:

>100_bases
TTCTGCTCGCGTTCGATGGCGCGCCAACCGATATCTTTCCGGCAGAAGCAATCATCCCAGTGGATATCGGTCATTAAGGC
GTAGGCGCGTTTCTGCGCTT

Product: transcriptional regulatory protein ZraR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGAQDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLGQSQDIQ
PLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Sequences:

>Translated_441_residues
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPV
LIMTAYSSVETAVEALKTGAQDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIP
LLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLGQSQDIQ
PLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR
>Mature_440_residues
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVL
IMTAYSSVETAVEALKTGAQDYLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPS
EATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLDE
IGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPL
LAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIPLGQSQDIQP
LVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=99.546485260771, Blast_Score=891, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=447, Percent_Identity=48.5458612975391, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1788905, Length=446, Percent_Identity=44.3946188340807, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790299, Length=462, Percent_Identity=41.991341991342, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI87082117, Length=315, Percent_Identity=48.5714285714286, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1789087, Length=313, Percent_Identity=46.6453674121406, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=46.9648562300319, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=42.4528301886792, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=42.9447852760736, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=42.8125, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1787583, Length=304, Percent_Identity=37.5, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=39.453125, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081858, Length=287, Percent_Identity=33.1010452961672, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789149, Length=112, Percent_Identity=33.9285714285714, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=119, Percent_Identity=32.7731092436975, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790860, Length=142, Percent_Identity=27.4647887323944, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786784, Length=124, Percent_Identity=27.4193548387097, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693157, Length=115, Percent_Identity=34.7826086956522, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=34.4262295081967, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786911, Length=131, Percent_Identity=30.5343511450382, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48398; Mature: 48267

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMA
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHH
LAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLTGEYISERELPLAIASTPIPLGQSQDIQPLVEVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
THDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQVREQVFDLVLCDVRMA
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQDYLIKPLDFDNLQATLEKA
HHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCHHHHHHHHHH
LAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAPSEATVLIHGDSGTGKELVAR
HHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
AIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAFTGADKRREGRFVEADGGTLFLD
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEEC
EIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVRLIAATHRDLAAEVNAGRFRQDLYY
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHE
RLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERNRKAVKGFTPQAMDLLIHYDWPGNIRE
EEEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCEEEEEEECCCCCHHH
LENAVERAVVLLTGEYISERELPLAIASTPIPLGQSQDIQPLVEVEKEVILAALEKTGGN
HHHHHHHEEEEEECCHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
KTEAARQLGITRKTLLAKLSR
HHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]