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The map label for this gene is insF1 [H]

Identifier: 30064320

GI number: 30064320

Start: 3091668

End: 3092534

Strand: Direct

Name: insF1 [H]

Synonym: S3210

Alternate gene names: 30064320

Gene position: 3091668-3092534 (Clockwise)

Preceding gene: 30064319

Following gene: 30064328

Centisome position: 67.22

GC content: 55.02

Gene sequence:

>867_bases
ATGAAGTATGTCTTTATTGAAAAACATCAGGCTGAGTTCAGCATCAAAGCAATGTGCCGCGTGCTCCGGGTGGCCCGCAG
CGGCTGGTATACGTGGTGTCAGCGGCGGACAAGGATAAGCACGCGTCAGCAGTTCCGCCAACACTGCGACAGCGTTGTCC
TCGCGGCTTTTACCCGGTCAAAACAGCGTTACGGTGCCCCACGCCTGACGGATGAACTGCGTGCTCAGGGTTACCCCTTT
AACGTAAAAACCGTGGCGGCAAGCCTGCGCCGTCAGGGACTGAGGGCAAAGGCCTCCCGGAAGTTCAGCCCGGTCAGCTA
CCGCGCACACGGCCTGCCTGTGTCAGAAAATCTGTTGGAGCAGGATTTTTACGCCAGTGGCCCGAACCAGAAGTGGCCAG
GAGACATCACGTACTTACGTACAGATGAAGGCTGGCTGTATCTGGCAGTGGTCATTGACCTGTGGTCACGTGCCGTTATT
GGCTGGTCAATGTCGCCACGCATGACGGCGCAACTGCCCTGCGATGCCCTGCAGATGGCGCTGTGGCGGCGTAAGAGGCC
CCGGAACGTTATCGTTCACACGGACCGTGGAGGCCAGTACTGTTCAGCAGATTATCAGGCGCAACTGAAACGGCATAATC
TGCGTGGAAGTATGAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAA
TGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCG
GTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACCAGAACCTTGCTTAG

Upstream 100 bases:

>100_bases
TGTCTACCGAGATTGCACGTCTCAAACGCCAGCTGGCAGAACGGGATGAAGAGCTGGCTATCCTCCAAAAGGCCGCGACA
TACTTCGCGAAGCGCCTGAA

Downstream 100 bases:

>100_bases
GCCTGTGTCCATATTACGTGGGTAGGATCACAAGACCTACTGCTATCACAATTACTGAGAGAAAAAAAAGCATCCGATGA
TTCATTTATGCTGTCCTAAA

Product: IS3 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA

Sequences:

>Translated_288_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA
>Mature_288_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLPCDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=242, Percent_Identity=33.8842975206612, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1788405, Length=288, Percent_Identity=98.9583333333333, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1787263, Length=288, Percent_Identity=98.9583333333333, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1786753, Length=288, Percent_Identity=98.9583333333333, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1786570, Length=288, Percent_Identity=98.9583333333333, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1786490, Length=288, Percent_Identity=98.9583333333333, Blast_Score=599, Evalue=1e-173,
Organism=Escherichia coli, GI1789981, Length=273, Percent_Identity=31.1355311355311, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33593; Mature: 33593

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRS
CCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLE
HHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCEEECCCCHHHHHHH
QDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLPCDALQMA
HHHHCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHH
LWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
HHHCCCCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA
EECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRS
CCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLE
HHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCEEECCCCHHHHHHH
QDFYASGPNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLPCDALQMA
HHHHCCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHH
LWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
HHHCCCCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEE
CIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA
EECCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]