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The map label for this gene is amiC [H]

Identifier: 30064159

GI number: 30064159

Start: 2902773

End: 2904116

Strand: Reverse

Name: amiC [H]

Synonym: S3025

Alternate gene names: 30064159

Gene position: 2904116-2902773 (Counterclockwise)

Preceding gene: 30064161

Following gene: 30064158

Centisome position: 63.14

GC content: 52.68

Gene sequence:

>1344_bases
ATGACAGATTATGCGTCTTTCGCTAAAGTTTCCGGTCAAATTAGTCGTTTACTTGTTACACAGCTTAGATTTCTTTTGCT
CGGGAGAGGCATGTCAGGATCCAACACTGCAATCAGCCGTCGTCGTTTACTGCAAGGCGCGGGCGCTATGTGGCTATTGA
GCGTAAGTCAGGTCAGCCTGGCTGCGGTTAGCCAGGTCGTGGCGGTGCGCGTCTGGCCTGCGTCCAGCTACACCCGCGTG
ACGGTAGAATCAAATCGTCAGCTGAAATATAAGCAGTTCGCGTTGAGTAACCCTGAACGCGTGGTGGTGGATATCGAAGA
TGTAAACCTGAACTCGGTACTCAAGGGGATGGCGGCACAAATTCGTGCAGACGACCCGTTCATCAAGTCGGCGCGCGTCG
GGCAATTTGACCCGCAAACCGTACGCATGGTTTTTGAATTAAAGCAAAACGTAAAACCGCAGCTGTTTGCCCTTGCGCCG
GTCGCCGGGTTTAAAGAGCGTCTGGTGATGGACCTCTATCCGGCCAATGCACAGGATATGCAGGACCCGCTGCTGGCGCT
GCTGGAGGATTACAACAAAGGCGACCTCGAAAAGCAGGTGCCGCCAGCGCAAAGTGGTCCACAACCGGGTAAAGCAGGGC
GCGATCGTCCGATTGTCATTATGCTTGACCCTGGCCACGGTGGCGAAGACTCCGGTGCGGTGGGGAAATACAAAACGCGC
GAAAAAGATGTGGTATTGCAAATAGCTCGCCGTTTGCGCTCTCTGATCGAGGAAGAGGGCAATATGAAGGTGTACATGAC
GCGCAATGAAGACATCTTCATTCCGTTGCAAGTGCGCGTAGCAAAAGCCCAGAAACAGCGTGCTGACCTGTTTGTCTCTA
TCCATGCCGACGCCTTTACCAGTCGTCAGCCGAGCGGTTCCTCGGTGTTTGCGCTCTCAACCAAAGGCGCAACCAGTACT
GCGGCAAAATATCTGGCACAAACCCAGAACGCCTCGGACTTGATTGGTGGCGTGAGCAAAAGCGGTGACCGCTATGTCGA
CCACACCATGTTCGATATGGTGCAGTCGCTGACTATTGCTGACAGCCTTAAGTTTGGTAAAGCGGTACTGAATAAGCTCG
GAAAAATCAACAAGCTGCATAAAAATCAAGTTGAACAGGCCGGGTTTGCCGTACTAAAGGCACCAGATATTCCCTCCATT
CTGGTCGAAACGGCGTTTATCAGTAACGTTGAGGAAGAGCGTAAACTGAAAACGGCGACTTTCCAGCAGGAAGTTGCGGA
GTCTATTCTTGCGGGGATTAAAGCGTATTTTGCCGATGGGGCAACGCTGGCGAGAAGGGGATGA

Upstream 100 bases:

>100_bases
TATTCTGTTTTTTCGCAGGGTGCAAGTGTAAATTTTATGCGAGAGCGACTTTTTTATCAGTAATGCCGTGAATATAAAAA
GAATGTTTACCGTTTATTAG

Downstream 100 bases:

>100_bases
TAAAAAGGCGCTGAATGGCGCTTTTTTTATGGGAGATCAGAAACAAAAAAACACCCGTTAGGGTGTTCAATAATTGGTTG
CGGGGGCCGGATTTGAACCG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTDYASFAKVSGQISRLLVTQLRFLLLGRGMSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRV
TVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAP
VAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTR
EKDVVLQIARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATST
AAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSI
LVETAFISNVEEERKLKTATFQQEVAESILAGIKAYFADGATLARRG

Sequences:

>Translated_447_residues
MTDYASFAKVSGQISRLLVTQLRFLLLGRGMSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRV
TVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAP
VAGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTR
EKDVVLQIARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATST
AAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSI
LVETAFISNVEEERKLKTATFQQEVAESILAGIKAYFADGATLARRG
>Mature_446_residues
TDYASFAKVSGQISRLLVTQLRFLLLGRGMSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPASSYTRVT
VESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPV
AGFKERLVMDLYPANAQDMQDPLLALLEDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTRE
KDVVLQIARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTA
AKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSIL
VETAFISNVEEERKLKTATFQQEVAESILAGIKAYFADGATLARRG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=417, Percent_Identity=99.7601918465228, Blast_Score=848, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=47.6595744680851, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=40.4444444444444, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48960; Mature: 48829

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYASFAKVSGQISRLLVTQLRFLLLGRGMSGSNTAISRRRLLQGAGAMWLLSVSQVSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
AAVSQVVAVRVWPASSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQ
HHHHHHHEEEEECCCCEEEEEEECCCCEEEHHHHCCCCCEEEEEEECCCHHHHHHHHHHH
IRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDM
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEEEECCCCHHHH
QDPLLALLEDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
EKDVVLQIARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFT
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHHHHHHCCCEEEEEECCHHC
SRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIA
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHH
FQQEVAESILAGIKAYFADGATLARRG
HHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TDYASFAKVSGQISRLLVTQLRFLLLGRGMSGSNTAISRRRLLQGAGAMWLLSVSQVSL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHH
AAVSQVVAVRVWPASSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQ
HHHHHHHEEEEECCCCEEEEEEECCCCEEEHHHHCCCCCEEEEEEECCCHHHHHHHHHHH
IRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDM
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEEEECCCCHHHH
QDPLLALLEDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
EKDVVLQIARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFT
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEHHHHHHHHCCCEEEEEECCHHC
SRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIA
CCCCCCCEEEEEECCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
DSLKFGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHH
FQQEVAESILAGIKAYFADGATLARRG
HHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]