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The map label for this gene is ygcY

Identifier: 30064133

GI number: 30064133

Start: 2873096

End: 2874436

Strand: Reverse

Name: ygcY

Synonym: S2995

Alternate gene names: 30064133

Gene position: 2874436-2873096 (Counterclockwise)

Preceding gene: 30064134

Following gene: 30064132

Centisome position: 62.5

GC content: 53.91

Gene sequence:

>1341_bases
ATGGCGACACAATCCAGTCCTGTTATTACTGATATGAAAGTCATTCCGGTCGCCGGACATGACAGCATGTTGCTCAATAT
TGGTGGTGCGCATAACGCATACTTCACCCGCAATATCGTGGTACTCACCGATAACGCCGGGCATACCGGCATTGGTGAAG
CGCCGGGCGGAGAGGTGATTTATCAAACACTGGTCGATGCTATTCCGATGGTTCTGGGCCAGGAAGTTGCGCGCCTGAAT
AAAGTGGTTCAGCAGGTACATAAAGGTAATCAGGCAGCCGATTTTGATACCTTTGGCAAAGGTGCCTGGACTTTTGAATT
GCGCGTTAATGCCGTGGCGGCGCTGGAAGCCGCCTTGCTTGACCTGCTAGGTAAGGCGCTGAATGTTCCGGTCTGCGAAC
TGTTGGGGCCAGGCAAGCAACGCGAGACTATTACCGTCCTCGGTTATCTGTTTTATATAGGTGATCGGACCAAAACCGAT
CTTCCTTATCTGGAAAATACGCCGGGCAACCATGAGTGGTATCAGTTGCGCCATCAGAAAGCGATGAACAGCGAAGCCGT
TGTGCGTCTGGCGGAAGCCTCACAGGATCGCTATGGCTTTAAAGATTTCAAACTTAAGGGCGGGGTGTTACCTGGCGAGC
AAGAAATCGACACTGTTCGTGCATTGAAGAAAAGCTTCCCGGATGCGCGGATTACCGTTGATCCCAACGGTGCATGGCTG
CTTGATGAAGCCATTTCTTTATGCAAAGGGCTGAATGATGTTCTTACCTATGCCGAAGATCCATGCGGCGCAGAACAGGG
CTTCTCCGGACGTGAAGTGATGGCGGAGTTTCGACGGGCGACCGGCTTGCCCGTCGCGACTAACATGATCGCCACCAACT
GGCGCGAAATGGGTCATGCGGTGATGCTCAATGCGGTAGATATTCCACTTGCCGATCCGCACTTCTGGACGCTTTCCGGT
GCAGTCCGCGTGGCGCAGCTTTGCGACGACTGGGGGCTGACCTGGGGCTGCCATTCCAACAACCATTTCGATATCTCTCT
GGCGATATTTACCCATGTGGGCGCGGCGGCACCGGGTAATCCTACCGCTATCGATACCCACTGGATTTGGCAGGAGGGCG
ATTGTCGCCTGACCAAAAATCCGCTGGAGATTAAAAACGGAAAAATTGCCGTTCCTGATGCGCCCGGTCTGGGCGTGGAA
CTGGACTGGGAACAGGTACAAAAGGCACATGAGGCCTATAAACGTCTGCCTTGCGGTGCGCGTAACGACGCAGGTCCGAT
GCAGTACCTGATCCCCGGCTGGACCTTTGACCGTAAACGTCCCGTTTTCGGCCGTCATTGA

Upstream 100 bases:

>100_bases
ATTTATGTTGGTGTTCATGCCCTAATCGCGGTACTGAGCTACCTGGTGCTGGTGGGCGATATCAAGCGTATCGAGCTGAA
ACCTGTCGCGGGGCAATAAG

Downstream 100 bases:

>100_bases
TTCTGAAAAAGGACATAAATATGAGTTCTCAATTTACGACGCCTGTTGTTACTGAAATGCAGGTCATCCCGGTGGCGGGT
CATGACAGTATGCTGATGAA

Product: putative glucarate dehydratase

Products: 5-dehydro-4-deoxy-D-glucarate; H2O

Alternate protein names: GDH-RP; GlucDRP [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLN
KVVQQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKSFPDARITVDPNGAWL
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSG
AVRVAQLCDDWGLTWGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVE
LDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH

Sequences:

>Translated_446_residues
MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLN
KVVQQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKSFPDARITVDPNGAWL
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSG
AVRVAQLCDDWGLTWGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVE
LDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH
>Mature_445_residues
ATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNK
VVQQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTDL
PYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKSFPDARITVDPNGAWLL
DEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGA
VRVAQLCDDWGLTWGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVEL
DWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH

Specific function: Does not seem to have an in-vivo activity on glucarate or idarate. Its real substrate is unknown [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789151, Length=446, Percent_Identity=98.4304932735426, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI1789150, Length=448, Percent_Identity=62.9464285714286, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.2.1.40

Molecular weight: Translated: 48867; Mature: 48736

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGHTGIGEAPGGEVI
CCCCCCCCEECEEEEEECCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHH
YQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
DLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTDLPYLENTPGNHEWYQLRHQK
HHHHHHHCCCHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
AMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKSFPDARITVDPNGAWL
HCCHHHHEEEHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCH
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA
HHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCE
VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAIFTHVGAAAPGN
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCCCC
PTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVELDWEQVQKAHEAYKRLPCGA
CCEEEEEEEEECCCCEEECCCCEECCCEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCC
RNDAGPMQYLIPGWTFDRKRPVFGRH
CCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTDNAGHTGIGEAPGGEVI
CCCCCCCEECEEEEEECCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHH
YQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGAWTFELRVNAVAALEAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
DLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTDLPYLENTPGNHEWYQLRHQK
HHHHHHHCCCHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
AMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKSFPDARITVDPNGAWL
HCCHHHHEEEHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCH
LDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA
HHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCE
VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAIFTHVGAAAPGN
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEECCCCCCCCC
PTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVELDWEQVQKAHEAYKRLPCGA
CCEEEEEEEEECCCCEEECCCCEECCCEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCC
RNDAGPMQYLIPGWTFDRKRPVFGRH
CCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucarate

Specific reaction: D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]