Click here to switch to the map view.

The map label for this gene is nrdE

Identifier: 30064042

GI number: 30064042

Start: 2769917

End: 2772022

Strand: Direct

Name: nrdE

Synonym: S2889

Alternate gene names: 30064042

Gene position: 2769917-2772022 (Clockwise)

Preceding gene: 30064041

Following gene: 30064043

Centisome position: 60.22

GC content: 51.95

Gene sequence:

>2106_bases
ATGGATTACCACGCGCTGAATGCGATGCTTAATCTTTACGATAGCGCAGGTCGCATTCAGTTCGATAAAGACCGCCAGGC
CGTTGACGCCTTTATTGCGACGCATGTGCGTCCGAACAGTGTGACCTTCAGTAGCCAGCAGCAGCGCCTGAACTGGCTGG
TCAACGAAGGTTACTATGATGAAAGCGTTCTTAATCGCTACTCTCGCGACTTTGTCATTACGCTGTTTGCCCACGCACAC
ACCAGCGGTTTTCGTTTCCAGACATTCCTCGGGGCATGGAAGTTTTACACCAGCTATACGTTGAAGACATTCGACGGTAA
ACGTTATCTGGAAGATTTTGCCGATCGAGTCACGATGGTGGCGCTGACGCTGGCACAAGGCGATGAGACGCTGGCGTTGC
AACTGACCGATGAAATGCTGTCAGGACGCTTTCAGCCAGCCACGCCAACATTCCTCAACTGCGGTAAGCAGCAGCGCGGC
GAACTGGTTTCCTGTTTTTTGCTGCGTATTGAAGACAATATGGAGTCGATTGGTCGGGCGGTAAATTCCGCACTGCAGCT
GTCGAAACGCGGCGGCGGCGTAGCATTTTTGCTGTCGAATCTGCGGGAAGCGGGCGCGCCAATTAAACGTATTGAAAATC
AATCTTCTGGCGTAATTCCGGTGATGAAAATGTTGGAAGACGCATTTTCCTATGCCAACCAACTCGGCGCGCGTCAGGGG
GCAGGTGCAGTCTATTTACATGCTCATCATCCCGATATTCTGCGTTTTCTCGACACGAAACGGGAAAATGCCGACGAAAA
AATCCGCATTAAAACACTGTCGCTGGGCGTGGTGATCCCGGATATCACTTTCCATCTGGCAAAAGAGAATGCGCAGATGG
CGCTGTTTTCGCCCTATGACGTTGAGCGAGTTTATGGCAAGCCGTTTGCCGATATCGCCATTAGCGAACACTATGACGAA
CTGGTTGCCGATGAACGCATCCGCAAAAAATACCTCAACGCCCGTGATTTCTTCCAGCGACTGGCAGAAATCCAGTTTGA
GTCCGGCTATCCCTACATCATGTATGAAGACACAGTAAACCGTGCTAACCCTATCGCCGGGCGCATAAATATGAGTAATC
TCTGCTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATGCCCGCACAGGCCATGATATT
TCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTTCCCGCACGGTAGAGACTGCCGTGCG
CGGTTTAACGGCGGTGTCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCA
TCGGACTGGGGCAGATGAATTTACATGGTTATCTGGCGAGGGAAGGCATCGCTTATGGTTCGCCGGAAGCCCTGGATTTC
ACCAATCTCTATTTCTATACCATCACCTGGCACGCACTGCGTACCTCGATGTTGCTGGCGCGCGAACGCGGTGAAACCTT
CGCCGGGTTCAAACAGTCACGCTATGCCAGTGGTGAATATTTCAGCCAATATCTGCAAGGTAACTGGCAGCCGAAAACGG
CGAAAGTTGGCGAACTGTTTGCCCGCAGCTGTATTACGTTACCTACCCGTGAGATGTGGGCGCAACTGCGCGACGACGTG
ATGCGCTACGGCATATACAACCAGAATCTCCAGGCGGTGCCGCCAACCGGGTCTATCTCTTATATCAACCATGCTACGTC
GAGTATTCATCCGATTGTGGCGAAAGTAGAGATACGCAAAGAGGGCAAAACAGGACGCGTTTACTACCCTGCCCCGTTTA
TGACTAACGAAAATCTGGCGCTGTATCAGGACGCTTACGAAATTGGCGCAGAAAAGATCATCGACACCTACGCGGAAGCG
ACTCGCCATGTCGATCAGGGGCTGTCGCTGACGCTGTTTTTCCCCGATACCGCCACCACTCGCGATATCAACAAAGCGCA
GATTTACGCCTGGCGCAAAGGTATCAAAACGCTCTATTACATCCGCCTGCGTCAGATGGCGCTGGAAGGCACTGAAATTG
AAGGCTGCGTCTCCTGTGCACTTTAA

Upstream 100 bases:

>100_bases
GTTTTGAACTCATGGGTACGCAAAGCGATATCGAAAACGTTCGTAAAGGAGTAACCGAATTTTGGCAACGACAACCGCAG
AATGCCTGACGCAGGAAACG

Downstream 100 bases:

>100_bases
GGAATATCTATGAAACTCTCACGTATCAGCGCCATCAACTGGAACAAGATATCTGACGATAAAGATCTGGAGGTGTGGAA
TCGCCTGACCAGCAATTTCT

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: R1E protein; Ribonucleotide reductase 2 [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MDYHALNAMLNLYDSAGRIQFDKDRQAVDAFIATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAH
TSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPATPTFLNCGKQQRG
ELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDAFSYANQLGARQG
AGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDE
LVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDI
SCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDF
TNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMWAQLRDDV
MRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEA
TRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Sequences:

>Translated_701_residues
MDYHALNAMLNLYDSAGRIQFDKDRQAVDAFIATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAH
TSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPATPTFLNCGKQQRG
ELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDAFSYANQLGARQG
AGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDE
LVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDI
SCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDF
TNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMWAQLRDDV
MRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEA
TRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
>Mature_701_residues
MDYHALNAMLNLYDSAGRIQFDKDRQAVDAFIATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAH
TSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPATPTFLNCGKQQRG
ELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDAFSYANQLGARQG
AGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDE
LVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDI
SCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDF
TNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMWAQLRDDV
MRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEA
TRHVDQGLSLTLFFPDTATTRDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R1E contains the binding sites for both substrates and allosteric effectors and carries out the actual reductio

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=669, Percent_Identity=27.3542600896861, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1789030, Length=701, Percent_Identity=98.8587731811698, Blast_Score=1452, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=582, Percent_Identity=25.7731958762887, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17555242, Length=642, Percent_Identity=25.7009345794392, Blast_Score=172, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6320914, Length=619, Percent_Identity=26.1712439418417, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6322123, Length=643, Percent_Identity=26.1275272161742, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI17352481, Length=646, Percent_Identity=23.8390092879257, Blast_Score=149, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 79115; Mature: 79115

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYHALNAMLNLYDSAGRIQFDKDRQAVDAFIATHVRPNSVTFSSQQQRLNWLVNEGYYD
CCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCCCC
ESVLNRYSRDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMV
HHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHH
ALTLAQGDETLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFLLRIEDNMESIGRA
HEEEECCCCEEEEEEEHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHHHHHHHHHHHHHHH
VNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDAFSYANQLGARQG
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
AGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYD
CCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEEEECCCC
VERVYGKPFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEECCCCCEEEEECCCC
RANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSR
CCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCEEEEECCCCCCHHH
TVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCCCHHHHHCCCCCCCCCCEEE
TNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELF
CEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHH
ARSCITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRK
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCCCCCCEEEEEEEEE
EGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATT
CCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
RDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MDYHALNAMLNLYDSAGRIQFDKDRQAVDAFIATHVRPNSVTFSSQQQRLNWLVNEGYYD
CCHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCCCC
ESVLNRYSRDFVITLFAHAHTSGFRFQTFLGAWKFYTSYTLKTFDGKRYLEDFADRVTMV
HHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHH
ALTLAQGDETLALQLTDEMLSGRFQPATPTFLNCGKQQRGELVSCFLLRIEDNMESIGRA
HEEEECCCCEEEEEEEHHHHCCCCCCCCCHHHHCCCHHHCCCEEHHHHHHHHHHHHHHHH
VNSALQLSKRGGGVAFLLSNLREAGAPIKRIENQSSGVIPVMKMLEDAFSYANQLGARQG
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
AGAVYLHAHHPDILRFLDTKRENADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYD
CCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEECCCCCEEEECCCC
VERVYGKPFADIAISEHYDELVADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVN
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEECCCCCEEEEECCCC
RANPIAGRINMSNLCSEILQVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSR
CCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHCCCCEEECCCCEEEEECCCCCCHHH
TVETAVRGLTAVSDMSHIRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDF
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEECCCCCCCHHHHHCCCCCCCCCCEEE
TNLYFYTITWHALRTSMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELF
CEEEEEEEEHHHHHHHHHHHHHCCCHHHCHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHH
ARSCITLPTREMWAQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRK
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHCCCCCCCEEEEEEEEE
EGKTGRVYYPAPFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATT
CCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
RDINKAQIYAWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8820648; 2158980 [H]