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The map label for this gene is ygaF

Identifier: 30064027

GI number: 30064027

Start: 2757111

End: 2758445

Strand: Direct

Name: ygaF

Synonym: S2872

Alternate gene names: 30064027

Gene position: 2757111-2758445 (Clockwise)

Preceding gene: 161486450

Following gene: 30064028

Centisome position: 59.95

GC content: 57.15

Gene sequence:

>1335_bases
GTGGCTATTTCGCTTACGCCACTCACCACTACCAGACGCATCAGTAAGCGCAAAGGAATTGAGCGGATGTATGATTTTGT
GATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTTTATCCGGATGCCCGCATTGCGTTGC
TGGAAAAAGAGTCCGGCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCC
GGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGGAACCGTGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTATGA
CAACTGCGGCAAGATGCTGGTCGCCACGTCTCAGCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGA
ACGGTATCGAGCGCGAGTGGATAAACGCCGTGGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTTGGTGGCATTTTT
GTGCCGTCCAGCGGCATTGTCAGCTACCGCGAAGTGACGGCGGCAATGGCAAAAATCTTCCAGGCCAGAGGCGGCGAGAT
TATTTATAACGCCGAAGTCAGCGCCCTCAGTGAGCATAAAAACGGCGTGGTGATACGCACCCGTCAGGGCGGTGAATATG
AAGCATCGACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGCCTCGAACCGGGCTTTATT
ATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATAACCAGATTGTTAACCACCTGATTTACCCCATTCC
CGACCCCGCGATGCCATTTTTGGGCGTGCATCTCACCCGCATGATCGACGGCAGCGTGACCGTCGGGCCAAACGCGGTGC
TGGCTTTCAAACGCGAAGGCTATCGCAAGCGCGACTTCTCATTTAGTGACACGCTGGAGATTTTGAGCTCGTCGGGGATT
CGCCGGGTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGCGGCTATCTGCGGCT
GGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCAGTATCGC
CGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCACGCACGATCCACACCTGCAATGCGCCCTCCCCGGCA
GCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACG
CACGCTGCGAGCGGCACGTAGTGTGGATGCCTTACACGCCGCATTTAATCAATAA

Upstream 100 bases:

>100_bases
CCCGTTGGCAAATTCCTGTTGATAAACAACCTGTTCTGGCTGCACGGTCGCGACCGCTTTACTCCGCACCCGGATCTGCG
CCGTGAACTGATGCGTCAGC

Downstream 100 bases:

>100_bases
CCTTTGAAAACAGGACGTAACAATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTTGATTAACGGGGAATGGC
TGGACGCCAACAATGGTGAA

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTP
GSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERMRALWERTAANGIEREWINAVELREREPNITGLGGIF
VPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILSSSGI
RRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPA
ATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ

Sequences:

>Translated_444_residues
MAISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTP
GSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERMRALWERTAANGIEREWINAVELREREPNITGLGGIF
VPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILSSSGI
RRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPA
ATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ
>Mature_443_residues
AISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPG
SLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERMRALWERTAANGIEREWINAVELREREPNITGLGGIFV
PSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFII
CPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILSSSGIR
RVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAA
TSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAFNQ

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=41.7085427135678, Blast_Score=322, Evalue=6e-88,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=97.867298578199, Blast_Score=842, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=43.3915211970075, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=39.5577395577396, Blast_Score=281, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 48592; Mature: 48461

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGP
CEEEECCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
ACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLE
CHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEHHHHH
MERMRALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIF
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHH
QARGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
HHCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEE
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREG
EECCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCC
YRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLA
CCCCCCCEEEEECCCCCEEHHEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SQSNPGRTLRAARSVDALHAAFNQ
CCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AISLTPLTTTRRISKRKGIERMYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGP
EEEECCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
ACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLE
CHHHCCCCCCEEEEEEEECCCCCEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEHHHHH
MERMRALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIF
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHH
QARGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
HHCCCCEEEECCHHHHHHCCCCEEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEE
ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREG
EECCCCCEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCC
YRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
QPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLA
CCCCCCCEEEEECCCCCEEHHEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
SQSNPGRTLRAARSVDALHAAFNQ
CCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]