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The map label for this gene is insG [H]

Identifier: 30063995

GI number: 30063995

Start: 2729727

End: 2730107

Strand: Reverse

Name: insG [H]

Synonym: S2833

Alternate gene names: 30063995

Gene position: 2730107-2729727 (Counterclockwise)

Preceding gene: 30064000

Following gene: 30063994

Centisome position: 59.36

GC content: 57.48

Gene sequence:

>381_bases
ATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCT
CGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGA
TGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAACCTCTTCACCAGATTGTGAATCGCCTGGACATCATGCTG
CCGGGCAATCGCCCCTTCGTTGCCCCAGTGCCGTTATTCAGGCCCGCCAGCGCCTGGGAAGTGAGGCTGTCCGCCGCGTG
TTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCACTGGTGCGGCCTGA

Upstream 100 bases:

>100_bases
GGCAGCTCAAGGGCTGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTC
ACTTGTTTCCGGATTTTTTT

Downstream 100 bases:

>100_bases
CCCTGCTGGCCATCGATGGTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACAT
GCCGGGAACCCGGCGCTCTA

Product: IS4 orf

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPVPLFRPASAWEVRLSAACSRKQRSSGITPRRIRTGAA

Sequences:

>Translated_126_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPVPLFRPASAWEVRLSAACSRKQRSSGITPRRIRTGAA
>Mature_126_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPVPLFRPASAWEVRLSAACSRKQRSSGITPRRIRTGAA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=126, Percent_Identity=76.984126984127, Blast_Score=187, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 14178; Mature: 14178

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHH
MALERKEPLHQIVNRLDIMLPGNRPFVAPVPLFRPASAWEVRLSAACSRKQRSSGITPRR
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
IRTGAA
HCCCCC
>Mature Secondary Structure
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHH
MALERKEPLHQIVNRLDIMLPGNRPFVAPVPLFRPASAWEVRLSAACSRKQRSSGITPRR
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
IRTGAA
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]