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The map label for this gene is yfiF

Identifier: 30063982

GI number: 30063982

Start: 2707976

End: 2709013

Strand: Reverse

Name: yfiF

Synonym: S2816

Alternate gene names: 30063982

Gene position: 2709013-2707976 (Counterclockwise)

Preceding gene: 30063988

Following gene: 30063980

Centisome position: 58.9

GC content: 56.55

Gene sequence:

>1038_bases
ATGAACGATGAAATGAAAGGTAAAAGCGGCAAGGTCAAAGTGATGTATGTCCGCAGTGATGATGATTCTGATAAACGTAC
CCACAACCCGCGTACCGGGAAAGGGGGCGGGCGTCCAGGAAAATCTCGTGCTGATGGTGGCCGTCGCCCCGCCCGCGATG
ACAAACAGAGTCAGCCCCGTGACCGCAAGTGGGAAGATTCGCCGTGGCGCACGGTTTCCCGCGCGCCGGGTGATGAGACG
CCGGAAAAGGCCGATCACGGTGGCATCAGTGGTAAAAGTTTTATTGATCCGGAAGTGTTGCGTCGTCAGCGTGCGGAAGA
AACCCGCGTCTACGGCGAAAACGCCTGTCAGGCACTGTTCCAGAGCCGTCCGGAAGCGATTGTTCGCGCCTGGTTTATCC
AGAGTGTAACGCCGCGTTTTAAAGAAGCCTTGCGCTGGATGGCAGCAAACCGCAAAGCGTACCATGTGGTGGATGAAGCG
GAATTGACAAAAGCGTCAGGTACGGAACATCACGGCGGCGTTTGCTTCCTGATCAAAAAGCGTAACGGTACAACCGTGCA
GCAGTGGGTAATCCAGGCAGGCGCGCAGGATTGCGTTCTGGCACTGGAAAACGAATCTAACCCGCATAACCTGGGTGGCA
TGATGCGCAGCTGCGCGCACTTCGGCGTGAAAGGTGTCGTGGTGCAGGATGCGGCACTGCTGGAGTCGGGGGCGGCTATC
CGTACCGCAGAAGGCGGCGCAGAGCACGTTCAGCCGATTACTGGCGACAACATTGTTAACGTGCTGGATGATTTCCGTCA
GGCAGGTTACACCGTAGTGACCACTTCCAGCGAGCAGGGTAAACCGCTGTTCAAAACCAGTCTGCCAGCGAAAATGGTAC
TGGTGCTGGGTCAGGAATATGAAGGGTTACCGGATGCCGCACGCGATCCGAACGATCTGCGCGTGAAGATTGACGGTACG
GGCAACGTTGCCGGGCTGAATATCTCTGTCGCAACCGGCGTTTTGCTTGGTGAATGGTGGCGTCAGAATAAAGCCTGA

Upstream 100 bases:

>100_bases
TCAATAGGTATGATAGTCGCAGGCTTTGCTACGAAATCATGGCGAACCGCGCGGACTTCGGGTAATCTGCGCGCTTCGCG
CAGCGCTGGTGGAGAAAAGC

Downstream 100 bases:

>100_bases
TAAATCAGCCGGGTGGCAACTCTGCCATCCGGCATTTCCCCGCAAATTTACTCACACTCTGCCGGTAATACTGGCATCCA
GTCAATCGGCGTCTCGCCAC

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Sequences:

>Translated_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA
>Mature_345_residues
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDET
PEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGT
GNVAGLNISVATGVLLGEWWRQNKA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=345, Percent_Identity=99.7101449275362, Blast_Score=711, Evalue=0.0,
Organism=Escherichia coli, GI1790623, Length=247, Percent_Identity=28.3400809716599, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 37811; Mature: 37811

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRADGGRRPARDDKQSQPR
CCCCCCCCCCCEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRRQRAEETRVYGENACQALF
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
QSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKK
HCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCEEEEEEC
RNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAI
CCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCEE
RTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEY
EECCCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHCCCCEEEEEECCCC
EGLPDAARDPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA
CCCCCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]