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The map label for this gene is yeiE

Identifier: 30063598

GI number: 30063598

Start: 2250670

End: 2251551

Strand: Reverse

Name: yeiE

Synonym: S2371

Alternate gene names: 30063598

Gene position: 2251551-2250670 (Counterclockwise)

Preceding gene: 30063602

Following gene: 30063597

Centisome position: 48.95

GC content: 53.51

Gene sequence:

>882_bases
ATGCACATCACCCTCCGGCAGTTGGAAGTTTTTGCAGAAGTATTGAAAAGTGGATCAACCACCCAGGCGTCGGTGATGCT
GGCGTTGTCGCAATCAGCAGTGAGCGCAGCCTTGACTGACCTGGAAGGGCAGCTTGGCGTTCAACTGTTTGATCGCGTGG
GGAAAAGACTGGTTGTTAATGAACACGGGCGGCTGCTCTATCCGCGAGCGTTGGCACTGCTTGAACAGGCGGTTGAAATC
GAACAACTGTTTCGCGAAGACAACGGCGCGATTCGTATCTATGCCAGTAGTACCATCGGTAACTACATTCTGCCTGCAGT
TATCGCCCGTTATCGCCATGATTATCCGCAGTTGCCGATTGAACTTAGCGTTGGGAACAGCCAGGACGTGATGCAAGCGG
TGCTGGATTTCCGCGTTGATATTGGCTTTATTGAAGGACCGTGCCACAGCACTGAAATCATTTCTGAACCGTGGCTGGAA
GACGAGCTGGTGGTTTTCGCCGCGCCGACTTCGCCGTTGGCTCGTGGTCCGGTCACCTTAGAACAGCTGGCCGCTGCGCC
GTGGATCCTGCGTGAACGCGGTTCCGGCACGCGGGATATTGTCGATTATCTGTTGCTGTCACATTTACCGAAGTTTGAGA
TGGCGATGGAATTAGGTAACTCCGAGGCAATCAAACATGCGGTGCGTCATGGGTTGGGAATTAGTTGCCTGTCGCGACGT
GTGATTGAAGATCAATTGCAGGCAGGCACATTAAGTGAAGTTGCGGTCCCTCTGCCGCGCCTGATGCGTACGTTGTGGCG
TATACATCATCGGCAAAAACACCTTTCCAACGCGCTACGGCGCTTTCTGGACTATTGCGATCCCGCAAATGTGCCGCGTT
AA

Upstream 100 bases:

>100_bases
ATAACCCTCTCCTTTAACCGGGCATAAGGTTACGACTAACTGGTTTAAAAATAAAATTGATTATATATTTATAATTAATC
TTTATAAGTGGTAAGCGACT

Downstream 100 bases:

>100_bases
GTTGCTGTACAAGAACATGCTGGTGCTGTGTCGATTTCGTGACGCAGCGCCTTCAGCATGCATTCGCCAGAAAAGAGATT
GGCTGCTTTACTTATAATCC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEI
EQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR

Sequences:

>Translated_293_residues
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEI
EQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR
>Mature_293_residues
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEI
EQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=99.6587030716723, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=25.8503401360544, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=27.681660899654, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787601, Length=198, Percent_Identity=29.2929292929293, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=28.6885245901639, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI145693105, Length=290, Percent_Identity=25.1724137931034, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787806, Length=180, Percent_Identity=31.1111111111111, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=206, Percent_Identity=29.126213592233, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=146, Percent_Identity=30.8219178082192, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787879, Length=301, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789204, Length=279, Percent_Identity=27.2401433691756, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=25.0996015936255, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789506, Length=197, Percent_Identity=27.4111675126904, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=24.8995983935743, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32710; Mature: 32710

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEC
EHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPI
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCE
ELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFAAPTSPLARGPVTL
EEECCCCHHHHHHHHHHHHHCCEECCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCH
EQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
HHHHCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFDRVGKRLVVN
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCEEEEC
EHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIARYRHDYPQLPI
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCE
ELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFAAPTSPLARGPVTL
EEECCCCHHHHHHHHHHHHHCCEECCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCH
EQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMELGNSEAIKHAVRHGLGISCLSRR
HHHHCCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
VIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFLDYCDPANVPR
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]