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The map label for this gene is recE [H]

Identifier: 30063286

GI number: 30063286

Start: 1894472

End: 1895896

Strand: Direct

Name: recE [H]

Synonym: S1955

Alternate gene names: 30063286

Gene position: 1894472-1895896 (Clockwise)

Preceding gene: 30063285

Following gene: 30063287

Centisome position: 41.19

GC content: 50.32

Gene sequence:

>1425_bases
ATGAACACCGATAAACAAGTTTACCCACTGTATTACGAAGCAAAAAATGACAAAGTAAGAAAACGTCTCGGTATTAAAGG
CGGTTTTTACTGGGCTGAAGCGAAAAAATTATCCATTGCCATCTCCCGTGGTGCTGTTGCGATTGACGATGCAGGCTACG
ATGAAGATGACTTTAAAAAACCTGTTCGCGTCAATTTGCCCGTTGTTGATGACCTCCCGCCAGAAGGCGTATTTGATACG
GAATTCTGCAACCGTTACGAAAAAGGCGGGGAAGATGGCATCACAATGGTATTTATCGCATCTTCTCCCTCTGTTCAGGA
CAAACCGGCCAGCACTGACAATACCAACGTCAACGGCGAAGACATGACAGAAATTGAGGAGAACATGCTCCTGCCAGTTT
CCGGTCAGGAACTGCCCATTCGCTGGCTTGCGCAACACGGCAGCGAAAAACCAGTAACGCACGTTTCACGCGACGAACTC
CAGGCATTACACATTGCTCGGGATGAAGAACTACCAGCTGTTACTGCCCTGGCTGTTTCCCACAAAACCAGCCTGCTCGA
TCCGCTGGAAATTCGCGATCTCCACAAACTGGTGCGTGATACTGACAGAGTTTTCCCTAATCCAGGCAATTCAAGCCTGG
GGCTGATGACTGCTTTTTTCGAAGCATACATGGACGCAGACTACACCGATCGCGGTCTGCTGACAAAAGAGTGGATGAAA
GGAAATCGTGTTTCACGCATCACGCGCACGGCTTCCGGCGCTAATGCTGGCGGCGGGAACCTCACCGATCGCGGCGAAGG
TTTCGTTCACGATCTGACGTCACTGGCGCGCGACGTAGCCACTGGCGTACTGGCTCGTTCAATGGACGTGGACATTTATA
ACCTTCATCCGGCACACGCTAAACGTGTCGAGGAAATTATCGCTGAAAATAAACCGCCCTTTTCTGTTTTCCGCGACAAA
TTCATCACCATGCCTGGCGGGATGGATTATTCCCGCGCCATCGTGGTTGCGTCAGTGAAAGAAGCACCGATTGGTATCGA
GGTCATCCCCGCTCACGTCACTGAATATCTGAACAAGGTACTGACTGAAACCGATCATGCCAACCCTGATCCGGAAATCG
TGGATATTGCCTGCGGTCGCTCCTCTGCCCCGATGCCGCAACGTGTAACAGAAGAAGGAAAACAGGATGATGAAGAAAAA
ACGCAGCCATCTGGCGCAATGGCAGATGAACAGGCAACGGCTGAAACAATGGAACCGGATGCAACTGAACATCATCAGAA
CACGCAGCCGCTGGATGCTCAGTCACAGGTAAATTCTGTTGATGAGAAATATCAGGAACTACGGGCAGAACTCCATGAAG
CCCGGAAAAACATTCCGCCCAAAAATCCTGTCGATGCAGACAAATTACTGGCTGCCTTGAGGTAG

Upstream 100 bases:

>100_bases
AAAAAAAGAAAGCCTGCACACTGTGCAGGCCTGAATGAAGAACCTGGGACATTTATTCATCACTCGCAGTAATTTTAATC
TGAGTTGAGGTTAAAAAACA

Downstream 100 bases:

>100_bases
CCTGTGAGGTGTACTGGCAATAGCGGACACTACCATTTGTTCTTTTTTTAAGCAGCCATCTGATGATATTTTTCCCTGAA
GGCTGCCGGGGAGATATTCC

Product: putative exodeoxyribonuclease VIII

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDT
EFCNRYEKGGEDGITMVFIASSPSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTDRGLLTKEWMK
GNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDK
FITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEK
TQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAALR

Sequences:

>Translated_474_residues
MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDT
EFCNRYEKGGEDGITMVFIASSPSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTDRGLLTKEWMK
GNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDK
FITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEK
TQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAALR
>Mature_474_residues
MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDT
EFCNRYEKGGEDGITMVFIASSPSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTDRGLLTKEWMK
GNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDK
FITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEK
TQPSGAMADEQATAETMEPDATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAALR

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=417, Percent_Identity=84.652278177458, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 52384; Mature: 52384

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDEDDFKK
CCCCCEEEEEEEECCCHHHHHHHCCCCCEEEECCCEEEEEEECCEEEECCCCCCHHHCCC
PVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTDNTNVNGE
CEEEECCEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
DMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARDEELPAVTALAVS
HHHHHHHCEEECCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
HKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTDRGLLTKEWMK
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSMDVDIYNLHPAHA
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHH
KRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKV
HHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHH
LTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKTQPSGAMADEQATAETMEPD
HHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHCCCC
ATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAALR
CHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDEDDFKK
CCCCCEEEEEEEECCCHHHHHHHCCCCCEEEECCCEEEEEEECCEEEECCCCCCHHHCCC
PVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTDNTNVNGE
CEEEECCEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
DMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARDEELPAVTALAVS
HHHHHHHCEEECCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
HKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTDRGLLTKEWMK
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHC
GNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSMDVDIYNLHPAHA
CCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHH
KRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKV
HHHHHHHHCCCCCHHHHHHHHEECCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHH
LTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKTQPSGAMADEQATAETMEPD
HHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHCCCC
ATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAALR
CHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]