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The map label for this gene is nemA

Identifier: 30063166

GI number: 30063166

Start: 1744351

End: 1745448

Strand: Direct

Name: nemA

Synonym: S1809

Alternate gene names: 30063166

Gene position: 1744351-1745448 (Clockwise)

Preceding gene: 30063165

Following gene: 30063167

Centisome position: 37.93

GC content: 53.55

Gene sequence:

>1098_bases
ATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAC
GCGTCTGCGCAGTATTGAACCGGGTGACATTCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTT
TGATTATTAGTGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAA
ATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCTTGCAGCTGTGGCACACCGGACG
CATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTC
TGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCGGGTATC
GTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGCTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTA
TTTGCTGCATCAGTTCCTTTCCCCTTCTTCAAACCATCGTACCGATCAGTACGGCGGCAGCGTGGAAAATCGCGCACGTT
TGGTACTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCGTTTCGCCAATCGGTACT
TTCCAGAACACAGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGC
TTATCTGCATATGTCAGAACCAGATTGGGCGGGGGGTGAACCGTATACTGATGCGTTCCGCGAAAAAGTACGCGCCCGTT
TCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCG
GTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCTCAAAGCTGAGCTTAACCCACAGCG
TGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAA

Upstream 100 bases:

>100_bases
TTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCA
CCAGACGACCGGGAGCCTTT

Downstream 100 bases:

>100_bases
TCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAG
ATGCGTCTTCTTCATACCAT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMALQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQLKAELNPQRAESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQ
IAAWKKITAGVHAENGHMALQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQLKAELNPQRAESFYGGGAEGYTDYPTL
>Mature_364_residues
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQI
AAWKKITAGVHAENGHMALQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIV
NDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGTF
QNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAV
AFGRDWIANPDLVARLQLKAELNPQRAESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=99.1780821917808, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=351, Percent_Identity=31.3390313390313, Blast_Score=117, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=356, Percent_Identity=29.7752808988764, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=28, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=374, Percent_Identity=28.3422459893048, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=363, Percent_Identity=26.7217630853994, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.1044776119403, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=353, Percent_Identity=28.8951841359773, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=216, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=38.560411311054, Blast_Score=232, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=36.7609254498715, Blast_Score=214, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39502; Mature: 39371

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDFRQAIANAREAAFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHHHHHHHH
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
HHHCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQLKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDFRQAIANAREAAFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCEECHHHCCCHHHHHHHHHHHHHHHHHHHHH
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRVSPIGT
HHHCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQLKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]