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The map label for this gene is pheS

Identifier: 30063019

GI number: 30063019

Start: 1586441

End: 1587424

Strand: Direct

Name: pheS

Synonym: S1634

Alternate gene names: 30063019

Gene position: 1586441-1587424 (Clockwise)

Preceding gene: 30063018

Following gene: 30063020

Centisome position: 34.49

GC content: 53.96

Gene sequence:

>984_bases
ATGTCACATCTCGCAGAACTGGTTGCCAGTGCGAAGGCGGCCATTAGCCAGGCGTCAGATGTTGCCGCGTTAGATAATGT
GCGCGTCGAATATTTGGGTAAAAAAGGGCACTTAACCCTTCAGATGACGACCCTGCGTGAGCTGCCGCCAGAAGAGCGTC
CGGCAGCTGGTGCGGTTATCAACGAAGCGAAAGAGCAGGTTCAGCAGGCGCTGAATGCGCGTAAAGCGGAACTGGAAAGC
GCTGCACTGAATGCGCGTCTGGCGGCGGAAACGATTGATGTCTCTCTGCCAGGTCGTCGCATTGAAAACGGCGGTCTGCA
TCCGGTTACCCGTACCATCGACCGTATCGAAAGTTTCTTCGGTGAGCTTGGCTTTACCGTGGCAACCGGGCCGGAAATCG
AAGACGATTATCATAACTTCGATGCTCTGAACATTCCTGGTCACCACCCGGCGCGCGCTGACCACGACACTTTCTGGTTT
GACGCTACCCGCCTGCTGCGTACCCAGACCTCTGGCGTACAGATCCGCACCATGAAAGCCCAGCAGCCACCGATTCGTAT
CATCGCGCCTGGCCGTGTTTATCGTAACGACTACGACCAGACTCACACGCCGATGTTCCATCAGATGGAAGGTCTGATTG
TTGATACCAACATCAGCTTTACCAACCTGAAAGGCACGCTGCACGACTTCCTGCGTAACTTCTTTGAGGAAGATTTGCAG
ATTCGCTTCCGTCCTTCCTACTTCCCGTTTACCGAGCCTTCTGCAGAAGTTGACGTCATGGGTAAAAACGGTAAATGGCT
GGAAGTGCTGGGCTGCGGGATGGTGCATCCGAACGTGCTGCGTAACGTTAGCATCGACCCGGAAGTTTACTCTGGTTTCG
CCTTCGGGATGGGGATGGAGCGTCTGACTATGTTGCGTTACGGCGTCACCGACCTGCGTTCATTCTTCGAAAACGATCTG
CGTTTCCTCAAACAGTTTAAATAA

Upstream 100 bases:

>100_bases
TCGAGCACTGCCCGGGACCAAAATGGCAAGTAAAATAGCCTGATGGGATAGGCTCTAAGTCCAACGAACCAGTGTCACCA
CTGACACAATGAGGAAAACC

Downstream 100 bases:

>100_bases
GGCAGGAATAGATTATGAAATTCAGTGAACTGTGGTTACGCGAATGGGTGAACCCGGCGATCGATAGCGATACGCTGGCG
AATCAAATCACTATGGCGGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVINEAKEQVQQALNARKAELES
AALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVINEAKEQVQQALNARKAELES
AALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDL
RFLKQFK
>Mature_326_residues
SHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVINEAKEQVQQALNARKAELESA
ALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFD
ATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQI
RFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLR
FLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=318, Percent_Identity=29.559748427673, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI4758340, Length=261, Percent_Identity=32.5670498084291, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=99.388379204893, Blast_Score=674, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32566635, Length=283, Percent_Identity=30.7420494699647, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=30.3846153846154, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=30.3846153846154, Blast_Score=121, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=30.3370786516854, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=90, Percent_Identity=38.8888888888889, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI18858079, Length=272, Percent_Identity=30.1470588235294, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17137424, Length=237, Percent_Identity=28.6919831223629, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 36832; Mature: 36701

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVI
CHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHCCCCCCCCCHHHH
NEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDATRLLRTQTSGVQIRTMKA
HHCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEC
QQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
CCCCEEEEECCHHHCCCCCCCCCCHHHHHCCEEEECCCCEEECHHHHHHHHHHHHHHCCE
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVSIDPEVYSGFAFGMGME
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHCCCCCHHHHCCHHHHCCHH
RLTMLRYGVTDLRSFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTLRELPPEERPAAGAVI
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEHHHHCCCCCCCCCHHHH
NEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIENGGLHPVTRTIDRIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDATRLLRTQTSGVQIRTMKA
HHCCCEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEC
QQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTNLKGTLHDFLRNFFEEDLQ
CCCCEEEEECCHHHCCCCCCCCCCHHHHHCCEEEECCCCEEECHHHHHHHHHHHHHHCCE
IRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLRNVSIDPEVYSGFAFGMGME
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHCCCCCHHHHCCHHHHCCHH
RLTMLRYGVTDLRSFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA