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The map label for this gene is pspE

Identifier: 30062828

GI number: 30062828

Start: 1363669

End: 1364016

Strand: Direct

Name: pspE

Synonym: S1395

Alternate gene names: 30062828

Gene position: 1363669-1364016 (Clockwise)

Preceding gene: 30062827

Following gene: 30062829

Centisome position: 29.65

GC content: 50.57

Gene sequence:

>348_bases
ATGGCAATTTTCATCCATAGAAGGACGCTTACATGTTTAAAAAAAGGCTTACTTGCTCTGGCACTGGTGTTTTCACTGCC
CGTTTTCGCCGCTGAACACTGGATCGATGTTCGTGTTCCAGAGCAGTATCAGCAAGAGCACGTTCAGGGGGCCATCAATA
TTCCCCTGAAGGAAGTGAAAGAGCGCATTGCCACCGCCGTTCCGGATAAAAACGACACCGTGAAAGTGTATTGCAATGCC
GGACGCCAGTCCGGGCAGGCAAAAGAGCTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGA
CATCGCAATGCCGAAGGTCAAAGGTTAA

Upstream 100 bases:

>100_bases
GCTGGAACCGCTGTTAAGTCGGGCTGCTAATAAACTGGCACAGCGTTATAAAAGGTGAGGGGAGTTTCGCAAAAATTGTT
AAATCTCAGGCGTATAATGG

Downstream 100 bases:

>100_bases
AAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCTATAGATTATTTGCGTC
AGCTCACAAATACGCTTTTT

Product: thiosulfate:cyanide sulfurtransferase

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 115; Mature: 114

Protein sequence:

>115_residues
MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNA
GRQSGQAKELLSEMGYTHVENAGGLKDIAMPKVKG

Sequences:

>Translated_115_residues
MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNA
GRQSGQAKELLSEMGYTHVENAGGLKDIAMPKVKG
>Mature_114_residues
AIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAG
RQSGQAKELLSEMGYTHVENAGGLKDIAMPKVKG

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=103, Percent_Identity=98.0582524271845, Blast_Score=211, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 12754; Mature: 12623

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVK
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCHHHHH
ERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENAGGLKDIAMPKVKG
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIPLKEVK
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCHHHHH
ERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENAGGLKDIAMPKVKG
HHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]