| Definition | Shigella flexneri 2a str. 2457T, complete genome. |
|---|---|
| Accession | NC_004741 |
| Length | 4,599,354 |
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The map label for this gene is topA
Identifier: 30062796
GI number: 30062796
Start: 1325199
End: 1327796
Strand: Direct
Name: topA
Synonym: S1361
Alternate gene names: 30062796
Gene position: 1325199-1327796 (Clockwise)
Preceding gene: 30062794
Following gene: 30062797
Centisome position: 28.81
GC content: 51.58
Gene sequence:
>2598_bases ATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTTGTGACTACGTGGT GAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCA AGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAG GCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAACTGGCTGAAAAAGCCGACCACATCTA TCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCATGGCACCTGCGGGAAGTGATTGGTGGTGATGATGCGCGCT ATAGCCGAGTGGTGTTTAACGAAATTACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGAT CGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTGTGGAAAAAGAT CGCTCGTGGTCTGTCTGCCGGTCGTGTGCAGTCGGTGGCGGTCCGCCTGGTGGTAGAGCGTGAGCGTGAAATTAAAGCGT TCGTACCGGAAGAGTTCTGGGAAGTCGATGCCAGCACGACTACGCCATCTGGTGAAGCGTTGGCGTTGCAGGTGACTCAT CAGAATGACAAACCGTTCCGTCCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAG CGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCA GCACCCGTCTTGGTTTTGGCGTGAAAAAAACCATGATGATGGCGCAGCGTTTGTATGAAGCAGGCTATATCACTTACATG CGTACCGACTCCACTAACCTGAGTCAGGACGCGGTAAATATGGTTCGCGGTTATATCAGCGATAATTTTGGTAAGAAATA TCTGCCGGAAAGTCCGAATCAGTACGCCAGCAAAGAAAACTCACAGGAAGCGCACGAAGCGATTCGTCCTTCTGACGTCA ATGTGATGGCGGAATCGCTGAAGGATATGGAAGCAGATGCGCAGAAACTGTACCAGTTAATCTGGCGTCAGTTCGTTGCC TGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCG TATTTTGCGCTTTGATGGCTGGACAAAAGTGATGCCTGCACTGCGTAAAGGCGATGAAGATCGTATCTTACCGGCAGTCG ATAAAGGCGATGCTCTGACGCTCGTTGAACTAACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCA TCGCTGGTTAAAGAACTGGAAAAACGTGGTATCGGTCGTCCGTCTACCTATGCGTCGATCATTTCGACCATTCAGGATCG TGGCTATGTGCGAGTAGAAAATCGTCGTTTCTATGCGGAAAAAATGGGCGAAATCGTCACCGATCGCCTTGAAGAAAATT TCCGCGAGTTAATGAACTACGACTTTACCGCGCAGATGGAAAACAGCCTCGACCAGGTGGCAAATCACGAAGCAGAGTGG AAAGCTGTACTGGATCACTTCTTCTCGGATTTCACCCAGCAGTTAGATAAAGCTGAAAAAGATCCGGAAGAGGGTGGTAT GCGCCCGAACCAGATGGTTCTGACCAGCATTGACTGCCCGACTTGTGGTCGCAAAATGGGGATTCGCACAGCGAGTACCG GGGTATTCCTTGGCTGTTCTGGCTATGCGCTGCCGCCGAAAGAGCGTTGCAAAACCACCATTAACCTGGTGCCGGAAAAC GAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCAC GGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGA TCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTG AAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAACACACGTAAGATTTTACGTAACGGTGA AGTGGCACCACCGAAAGAAGATCCGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTG ACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTT TATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGAC CATGGTTCGCTTTAGCCGTAAAACCAAACAGCAATATGTCTCTTCGGAAAAAGACGGAAAGGCGACTGGCTGGTCAGCAT TTTATGTTGATGGCAAATGGGTTGAAGGAAAAAAATAA
Upstream 100 bases:
>100_bases ATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAA TCAAATTAGGTAAGGTGAAT
Downstream 100 bases:
>100_bases CCTTTAATTCTGTCAGGTTTTTATAAACAAAGGGTCGCGAAAGCGGCCCTTTTTTATTGCATATTATTTTTTCTTCACAC CTATACACTAAGGCTATAAA
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 865; Mature: 864
Protein sequence:
>865_residues MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWE AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM RTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVA CQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEW KAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN EVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL YRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
Sequences:
>Translated_865_residues MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWE AHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM RTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVA CQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEW KAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPEN EVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL YRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK >Mature_864_residues GKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEA HYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDR VNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTHQ NDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMR TDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVAC QMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEAS LVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWK AVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENE VLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLK MGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELY RFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYVDGKWVEGKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=609, Percent_Identity=25.2873563218391, Blast_Score=148, Evalue=2e-35, Organism=Homo sapiens, GI4507635, Length=514, Percent_Identity=27.431906614786, Blast_Score=148, Evalue=3e-35, Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=99.7687861271676, Blast_Score=1799, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=549, Percent_Identity=23.6794171220401, Blast_Score=122, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17555378, Length=505, Percent_Identity=25.5445544554455, Blast_Score=130, Evalue=4e-30, Organism=Caenorhabditis elegans, GI32563869, Length=565, Percent_Identity=24.4247787610619, Blast_Score=128, Evalue=2e-29, Organism=Drosophila melanogaster, GI24585251, Length=618, Percent_Identity=24.2718446601942, Blast_Score=137, Evalue=4e-32, Organism=Drosophila melanogaster, GI24640096, Length=508, Percent_Identity=25.9842519685039, Blast_Score=122, Evalue=1e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 97368; Mature: 97237
Theoretical pI: Translated: 8.51; Mature: 8.51
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.7 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 1.7 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 4.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK CCCEEEEEECCCHHHHHHHHHCCCHHHHHCCCHHHCCCCCCCHHHCCCCCCCCHHCCCCC KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA CCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH WHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEE QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE RLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHH SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC DFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL CCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH SSEKDGKATGWSAFYVDGKWVEGKK CCCCCCCCCCEEEEEECCEECCCCC >Mature Secondary Structure GKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAAKKSADSTSTKTAKKPK CCEEEEEECCCHHHHHHHHHCCCHHHHHCCCHHHCCCCCCCHHHCCCCCCCCHHCCCCC KDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIA CCHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH WHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFMDRVVGYMVS HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH PLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWEVDASTTTPSGEALALQVTH HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEEE QNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPTTSKPGAPFITSTLQQAASTRLGFG CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC VKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASKEN HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCC SQEAHEAIRPSDVNVMAESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDF HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCE RLKARGRILRFDGWTKVMPALRKGDEDRILPAVDKGDALTLVELTPAQHFTKPPARFSEA EEEECCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHHHCCCCCCCHHHH SLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNY HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC DFTAQMENSLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCP CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCC TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPENEVLNVLEGEDAETNALRAKR CCCHHHCCEEECCCEEEECCCCCCCCHHHCCCEEEECCCHHHHHHHCCCCCHHHHHHHHH RCPKCGTAMDSYLIDPKRKLHVCGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHL CCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCCHHHHHCCCHHHH KMGRFGKYMACTNEECKNTRKILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF HHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYV EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH SSEKDGKATGWSAFYVDGKWVEGKK CCCCCCCCCCEEEEEECCEECCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]