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The map label for this gene is sohB

Identifier: 30062794

GI number: 30062794

Start: 1323483

End: 1324532

Strand: Direct

Name: sohB

Synonym: S1359

Alternate gene names: 30062794

Gene position: 1323483-1324532 (Clockwise)

Preceding gene: 30062791

Following gene: 30062796

Centisome position: 28.78

GC content: 50.57

Gene sequence:

>1050_bases
GTGGAATTGTTGTCTGAATATGGTTTGTTTTTGGCTAAAATCGTTACCGTTGTGCTAGCGATTGCGGCGATTGCCGCCAT
TATTGTCAATGTTGCTCAACGTAATAAACGCCAGCGTGGCGAGTTACGGGTCAACAATCTCAGCGAACAGTATAAGGAGA
TGAAAGAAGAACTGGCCGCGGCGCTGATGGACTCACATCAGCAAAAACAGTGGCACAAAGCGCAGAAGAAAAAGCACAAG
CAAGAAGCGAAAGCAGCAAAAGCGAAAGCCAAACTGGGCGAGGTGGCAACTGACAGTAAACCTCGCGTCTGGGTGCTGGA
TTTTAAAGGCAGCATGGACGCCCATGAAGTGAACTCGCTACGTGAAGAGATAACGGCGGTACTCGCAGCATTCAAACCGC
AGGATCAGGTTGTGCTCCGTCTGGAAAGCCCTGGTGGCATGGTGCATGGTTACGGGTTGGCGGCTTCGCAGCTGCAGCGT
CTGCGTGATAAAAACATTCCTTTAACTGTTACGGTAGACAAAGTCGCTGCCAGCGGCGGTTACATGATGGCCTGTGTGGC
GGACAAAATTGTTTCCGCACCGTTTGCTATTGTGGGTTCCATTGGGGTGGTGGCGCAAATGCCCAACTTTAACCGCTTCC
TGAAAAGCAAAGATATTGATATCGAACTGCACACCGCCGGGCAGTATAAGCGCACGCTGACCTTGCTGGGTGAAAATACC
GAAGAAGGGCGGGAGAAATTCCGCGAAGAGCTGAACGAAACGCATCAGTTATTTAAAGATTTTGTGAAGCGTATGCGTCC
GTCTCTGGATATTGAACAGGTGGCAACGGGTGAACACTGGTACGGACAACAGGCGGTAGAGAAAGGCCTGGTTGATGAAA
TCAACACCAGTGATGAAGTTATTCTTAGCCTGATGGAAGGCCGTGAAGTGGTTAATGTACGCTATATGCAGCGTAAACGA
CTCATTGACCGATTCACCGGCAGCGCGGCAGAGAGCGCCGATCGATTGTTGCTACGCTGGTGGCAGCGGGGTCAAAAGCC
ATTGATGTAA

Upstream 100 bases:

>100_bases
ACTTTGTCATACTTTCGCTGCAATAACATCTCTGCGAGACGGCTTAACATGCCTGTTGTAAACTGTGAGCCAAAGCGTTG
TTTAACCAAGGTGGGGACTC

Downstream 100 bases:

>100_bases
AAGACAAACGCGAGGCTAAGACCTCGCGTTTTGCTTTAATCAACCAGATGATATTTTTCTGAAAGCACATGGGCCAGGTG
TTTGAACATATTAAACACCG

Product: putative periplasmic protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRVNNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHK
QEAKAAKAKAKLGEVATDSKPRVWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKDIDIELHTAGQYKRTLTLLGENT
EEGREKFREELNETHQLFKDFVKRMRPSLDIEQVATGEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKR
LIDRFTGSAAESADRLLLRWWQRGQKPLM

Sequences:

>Translated_349_residues
MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRVNNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHK
QEAKAAKAKAKLGEVATDSKPRVWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKDIDIELHTAGQYKRTLTLLGENT
EEGREKFREELNETHQLFKDFVKRMRPSLDIEQVATGEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKR
LIDRFTGSAAESADRLLLRWWQRGQKPLM
>Mature_349_residues
MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRVNNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHK
QEAKAAKAKAKLGEVATDSKPRVWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKDIDIELHTAGQYKRTLTLLGENT
EEGREKFREELNETHQLFKDFVKRMRPSLDIEQVATGEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKR
LIDRFTGSAAESADRLLLRWWQRGQKPLM

Specific function: Multicopy suppressor of the htrA (degP) null phenotype. It is possibly a protease, not essential for bacterial viability

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family

Homologues:

Organism=Escherichia coli, GI1787527, Length=349, Percent_Identity=100, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI1788064, Length=170, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SOHB_ECOLI (P0AG14)

Other databases:

- EMBL:   M73320
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64875
- RefSeq:   AP_001898.1
- RefSeq:   NP_415788.1
- ProteinModelPortal:   P0AG14
- SMR:   P0AG14
- DIP:   DIP-35930N
- STRING:   P0AG14
- MEROPS:   S49.002
- EnsemblBacteria:   EBESCT00000000384
- EnsemblBacteria:   EBESCT00000000385
- EnsemblBacteria:   EBESCT00000015999
- GeneID:   945858
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1264
- KEGG:   eco:b1272
- EchoBASE:   EB0949
- EcoGene:   EG10956
- eggNOG:   COG0616
- GeneTree:   EBGT00050000009416
- HOGENOM:   HBG366205
- OMA:   LEETHVL
- ProtClustDB:   PRK11778
- BioCyc:   EcoCyc:EG10956-MONOMER
- Genevestigator:   P0AG14
- GO:   GO:0006508
- InterPro:   IPR002142
- InterPro:   IPR013703

Pfam domain/function: PF01343 Peptidase_S49; PF08496 Peptidase_S49_N

EC number: 3.4.21.- [C]

Molecular weight: Translated: 39367; Mature: 39367

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: ACT_SITE 178-178

Signals:

None

Transmembrane regions:

HASH(0x123ea68c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRVNNLSEQYKEMKEELAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPRVWVLDFKGSMDAHEVNSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
REEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQRLRDKNIPLTVTVDKVAASGG
HHHHHHHHHHCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCEEEEEHHHHCCCC
YMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKDIDIELHTAGQYKRTLTLLGENT
EEHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCHHHHHHEECCCC
EEGREKFREELNETHQLFKDFVKRMRPSLDIEQVATGEHWYGQQAVEKGLVDEINTSDEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCHHHHCCCHHHH
ILSLMEGREVVNVRYMQRKRLIDRFTGSAAESADRLLLRWWQRGQKPLM
HHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRVNNLSEQYKEMKEELAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
ALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPRVWVLDFKGSMDAHEVNSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHH
REEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQRLRDKNIPLTVTVDKVAASGG
HHHHHHHHHHCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHCCCCEEEEEHHHHCCCC
YMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKDIDIELHTAGQYKRTLTLLGENT
EEHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCCHHHHHHEECCCC
EEGREKFREELNETHQLFKDFVKRMRPSLDIEQVATGEHWYGQQAVEKGLVDEINTSDEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHCCHHHHCCCHHHH
ILSLMEGREVVNVRYMQRKRLIDRFTGSAAESADRLLLRWWQRGQKPLM
HHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1885549; 9097039; 9278503; 3029379