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The map label for this gene is potC

Identifier: 30062655

GI number: 30062655

Start: 1172174

End: 1172968

Strand: Reverse

Name: potC

Synonym: S1206

Alternate gene names: 30062655

Gene position: 1172968-1172174 (Counterclockwise)

Preceding gene: 161486506

Following gene: 30062654

Centisome position: 25.5

GC content: 49.94

Gene sequence:

>795_bases
ATGATCGGTCGACTGCTTCGCGGCGGTTTTATGACCGCTATCTACGCGTACCTGTATATCCCAATCATTATTTTGATTGT
GAACTCCTTTAACAGCTCGCGCTTTGGCATCAACTGGCAGGGTTTTACCACCAAATGGTATAGCCTGCTGATGAACAACG
ACAGCCTGTTACAGGCAGCGCAGCATTCACTAACAATGGCGGTGTTTTCGGCGACGTTTGCTACGCTTATCGGTTCACTG
ACGGCAGTTGCGCTGTACCGTTATCGCTTTCGCGGTAAGCCGTTCGTTAGCGGAATGCTGTTTGTGGTAATGATGTCACC
AGATATCGTGATGGCAATTTCGCTGCTGGTGCTGTTTATGCTGCTGGGTATTCAGCTTGGCTTCTGGTCGCTGCTGTTCT
CGCATATCACCTTCTGCCTGCCATTTGTGGTGGTGACGGTGTATTCGCGCCTGAAAGGTTTTGACGTGCGGATGCTGGAA
GCGGCGAAAGATCTCGGTGCCAGCGAATTTACCATTCTGCGAAAAATCATTCTGCCACTGGCAATGCCAGCGGTGGCGGC
GGGCTGGGTGTTAAGCTTTACCCTGTCGATGGACGATGTGGTAGTTTCTTCATTCGTCACCGGACCAAGTTATGAAATTC
TGCCGTTAAAAATTTATTCGATGGTCAAAGTCGGCGTATCGCCGGAAGTTAACGCGCTGGCAACCATATTACTGGTGCTG
TCGCTGGTGATGGTTATTGCCAGCCAGCTTATTGCTCGTGATAAAACGAAAGGTAACACAGGGGACGTTAAATGA

Upstream 100 bases:

>100_bases
TTGGTGCAGCCACCAGCATTACGCTGACTATCGTAATGGGCCTGATGTTGCTGGTTTACTGGCGCGCTTCTCGTTTGCTG
AATAAGAAGGTGGAACTCGA

Downstream 100 bases:

>100_bases
AAAAATGGTCACGCCACCTGCTCGCGGCGGGTGCTCTGGCACTGGGCATGAGCGCCGCTCACGCCGATGACAACAACACG
CTGTATTTCTACAACTGGAC

Product: spermidine/putrescine ABC transporter membrane protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTTKWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSL
TAVALYRYRFRGKPFVSGMLFVVMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKIYSMVKVGVSPEVNALATILLVL
SLVMVIASQLIARDKTKGNTGDVK

Sequences:

>Translated_264_residues
MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTTKWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSL
TAVALYRYRFRGKPFVSGMLFVVMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKIYSMVKVGVSPEVNALATILLVL
SLVMVIASQLIARDKTKGNTGDVK
>Mature_264_residues
MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTTKWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSL
TAVALYRYRFRGKPFVSGMLFVVMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKIYSMVKVGVSPEVNALATILLVL
SLVMVIASQLIARDKTKGNTGDVK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1787368, Length=264, Percent_Identity=100, Blast_Score=526, Evalue=1e-151,
Organism=Escherichia coli, GI1787081, Length=251, Percent_Identity=40.2390438247012, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787714, Length=246, Percent_Identity=29.2682926829268, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788764, Length=207, Percent_Identity=28.0193236714976, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787713, Length=116, Percent_Identity=31.0344827586207, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): POTC_ECO57 (P0AFK8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90816
- PIR:   H85675
- RefSeq:   NP_287256.1
- RefSeq:   NP_309527.1
- ProteinModelPortal:   P0AFK8
- EnsemblBacteria:   EBESCT00000025222
- EnsemblBacteria:   EBESCT00000057441
- GeneID:   912458
- GeneID:   959476
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1763
- KEGG:   ecs:ECs1500
- GeneTree:   EBGT00050000008926
- HOGENOM:   HBG685300
- OMA:   HITFSIP
- ProtClustDB:   PRK09500
- BioCyc:   ECOL83334:ECS1500-MONOMER
- InterPro:   IPR000515

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 29111; Mature: 29111

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x119b0604)-; HASH(0x11d05af0)-; HASH(0x12074154)-; HASH(0x11c42cc8)-; HASH(0xf8bb59c)-; HASH(0x10ce96a4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796