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The map label for this gene is macA

Identifier: 30062366

GI number: 30062366

Start: 865361

End: 866476

Strand: Direct

Name: macA

Synonym: S0878

Alternate gene names: 30062366

Gene position: 865361-866476 (Clockwise)

Preceding gene: 30062364

Following gene: 30062367

Centisome position: 18.81

GC content: 53.41

Gene sequence:

>1116_bases
ATGAAAAAGCGGAAAACCGTGAAGAAGCGTTACGTTATTGCGCTGGTGATAGTCATCGCCGGACTGATTACGTTATGGAG
AATTCTTAACGCACCCGTGCCGACTTATCAGACACTGATTGTGCGCCCCGGTGATTTACAGCAAAGCGTGCTGGCGACCG
GAAAGCTGGACGCGCTGCGTAAGGTTGACGTGGGCGCGCAGGTCAGCGGTCAGTTGAAAACGCTGTCGGTGGCGATTGGC
GATAAAGTAAAAAAAGACCAGCTTTTAGGGGTCATTGATCCTGAACAGGCTGAAAACCAGATCAAGGAGGTCGAAGCAAC
GCTGATGGAGCTACGCGCGCAGCGGCAGCAGGCGGAAGCGGAGCTGAAACTGGCGCGGGTGACGTATTCCCGTCAGCAAC
GTCTGGCACAAACGCAAGCGGTTTCACTGCAGGATCTCGATACCGCCGCGACGGAGATGGCTGTGAAACAGGCGCAAATT
GGCACCATTGACGCGCAAATCAAGCGCAATCAGGCCTCTCTCGATACGGCTAAAACCAATCTCGATTACACCCGTATCGT
TGCCCCGATGGCTGGGGAAGTGACGCAAATCACCACTCTGCAAGGCCAGACGGTGATTGCCGCACAGCAAGCACCGAACA
TTCTGACGCTGGCAGATATGAGCACTATGCTGGTAAAAGCGCAGGTTTCTGAAGCGGATGTAATCCACCTGAAGCCGGGG
CAAAAAGCCTGGTTTACGGTGCTTGGCGATCCACTGACGCGCTACGAGGGGCAAATCAAGGATGTGCTACCGACGCCGGA
AAAGGTAAACGACGCTATTTTCTATTACGCCCGTTTTGAAGTACCCAACCCCAATGGTTTGCTGCGGCTGGATATGACCG
CGCAAGTGCATATTCAGCTCACCGATGTGAAAAATGTGTTGACGATCCCTCTGTCGGCGTTAGGCGATCCGGTTGGCGAT
AATCGTTATAAAGTCAAATTGTTGCGTAATGGTGAAACACGCGAGCGTGAAGTGACGATTGGCGCACGTAACGATACCGA
TGTTGAGATTGTCAAAGGGCTGGAAGCGGGCGATGAAGTGGTGATTGGTGAGGCCAAACCAGGAGCTGCACAATGA

Upstream 100 bases:

>100_bases
AAACATCAATAGTGTGATTACTAACGTAAATTTTAGGGTTTTGTTGATATTTCGTTGAAGTTAATGACCCGGATTGGCAT
ATGGAGTATTCAGAATATTT

Downstream 100 bases:

>100_bases
CGCCTTTGCTCGAATTAAAGGATATTCGTCGCAGCTATCCTGCCGGTGATGAGCAGGTTGAGGTGCTGAAGGGCATCAGC
CTCAATATTTATGCGGGTGA

Product: macrolide transporter subunit MacA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIG
DKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPG
QKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGD
NRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ

Sequences:

>Translated_371_residues
MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIG
DKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPG
QKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGD
NRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ
>Mature_371_residues
MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIG
DKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPG
QKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGD
NRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ

Specific function: Efflux transporter for macrolide antibiotics

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family

Homologues:

Organism=Escherichia coli, GI87081796, Length=371, Percent_Identity=99.1913746630728, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI87082036, Length=316, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789929, Length=346, Percent_Identity=24.2774566473988, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787013, Length=314, Percent_Identity=25.4777070063694, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MACA_ECO57 (P64176)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90749
- PIR:   H85599
- RefSeq:   NP_286650.2
- RefSeq:   NP_308991.2
- ProteinModelPortal:   P64176
- SMR:   P64176
- EnsemblBacteria:   EBESCT00000029022
- EnsemblBacteria:   EBESCT00000055877
- GeneID:   917702
- GeneID:   958411
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1115
- KEGG:   ecs:ECs0964
- GeneTree:   EBGT00050000009284
- HOGENOM:   HBG651049
- OMA:   AYGVFRI
- ProtClustDB:   PRK11578
- BioCyc:   ECOL83334:ECS0964-MONOMER
- InterPro:   IPR006143
- TIGRFAMs:   TIGR01730

Pfam domain/function: PF00529 HlyD

EC number: NA

Molecular weight: Translated: 40640; Mature: 40640

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCHHHHH
KVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEA
HCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQIGTIDAQIKRNQASLDTAKTN
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEHHEECCCHHHHHHHCC
LDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPG
CCHHEEECCCCCCEEEEEEECCCEEEEECCCCCEEEEHHHHHHHEEEECCCCCEEEECCC
QKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQL
CCEEEEEECCHHHHHCCCHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEEEEEE
TDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEV
ECCCCEEEEEHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHEECCCCCCEE
VIGEAKPGAAQ
EEECCCCCCCC
>Mature Secondary Structure
MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHCCHHHHH
KVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEA
HCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQIGTIDAQIKRNQASLDTAKTN
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEHHEECCCHHHHHHHCC
LDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPG
CCHHEEECCCCCCEEEEEEECCCEEEEECCCCCEEEEHHHHHHHEEEECCCCCEEEECCC
QKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQL
CCEEEEEECCHHHHHCCCHHHCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEEEEEE
TDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEV
ECCCCEEEEEHHHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCHHHEECCCCCCEE
VIGEAKPGAAQ
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796