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The map label for this gene is ybeF

Identifier: 30062182

GI number: 30062182

Start: 685528

End: 686328

Strand: Direct

Name: ybeF

Synonym: S0674

Alternate gene names: 30062182

Gene position: 685528-686328 (Clockwise)

Preceding gene: 161486530

Following gene: 30062183

Centisome position: 14.9

GC content: 46.94

Gene sequence:

>801_bases
GTGCTTAATCTGACCCCCTCGGCAATCAGTCAGTCTATTCAGAAACTGCGCGTTATATTCCCTGACCCATTGTTTATTCG
CAAAGGCCAGGGTGTCACTCCTACCGCATTTGCGATGCATCTACATGAGTATATCAGTCAGGGCCTTGAGTCCATTCTTG
GCGCGCTGGATATCGAAGGAAGCTATGATAAGCAACGAACGATAACTATTGCGACCACTCCCTCGGTCGGAGCCCTGGTC
CTTCCTGTCATCTATCGGGCGATTAAAACTCACTATCCGCAGCTTTTACTGCGCAACCCGCCCATCAGCGACGCGGAAAA
CCAACTCAGTCAGTTTCAAACCGATCTCATCATCGATAACATGTTTTGCACCAATCGTACGGTGCAACATCATGTTCTGT
TCACCGACAATATGGTGTTAATTTGCCGCAAGGGAAATCCACTACTCTCTTTAGAAGATGACAGAGAGACTATCGACAAC
GCTGCGCATGTACTTCTGTTACCGGAAGGGCAAAATTTCAGCGGTCTGCGGCAGAGAGTTCAAGAGATGTTTCCGGACCG
GCAAATCAATTTCACCAGCTACAACATTTTGACAATCGCTGCACTGGTTGCCAACAGTGACATGTTAGCGATTATTCCCA
GCCGTTTTTATAACCTGTTTAGTCGTTGCTGGCCGCTGGAAAAACTGCCTTTTCCGTCCTTAAATGAGGAGCAAATAGAT
TTCTCTATCCACTACAACAAATTCATCCTGCGTGATCCCATCCTGCACGGAGTAATAGATGTTATCCGTAATGCATTTTG
A

Upstream 100 bases:

>100_bases
TAAACCACAAATATTTACAACCTTACGAAATATTGATCTTAACCTTCTGACTATTTTTGAAGCTGTATATGTACATAAAG
GGATCGTTAATGCAGCGAAA

Downstream 100 bases:

>100_bases
AAAAGTATTGTTACAGCCAGATCACAGCGCAGAACGATAAAATGGCACAATCCAACAACAATTTTACATTTTCGCGACCG
CTTTGGCTGCTTTCAGGTCC

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALV
LPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDN
AAHVLLLPEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQID
FSIHYNKFILRDPILHGVIDVIRNAF

Sequences:

>Translated_266_residues
MLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALV
LPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDN
AAHVLLLPEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQID
FSIHYNKFILRDPILHGVIDVIRNAF
>Mature_266_residues
MLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALV
LPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDN
AAHVLLLPEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQID
FSIHYNKFILRDPILHGVIDVIRNAF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081766, Length=266, Percent_Identity=98.4962406015038, Blast_Score=539, Evalue=1e-155,
Organism=Escherichia coli, GI1786820, Length=272, Percent_Identity=27.5735294117647, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081684, Length=238, Percent_Identity=23.109243697479, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790147, Length=287, Percent_Identity=23.3449477351916, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30245; Mature: 30245

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEG
CCCCCHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDN
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHC
MFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLRQRV
HHHCCCCEEEEEEEECCEEEEEECCCCCEEECCHHHHHCCCEEEEEEECCCCHHHHHHHH
QEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQID
HHHCCCCEECEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
FSIHYNKFILRDPILHGVIDVIRNAF
EEEEECEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDIEG
CCCCCHHHHHHHHHHHHEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDN
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHC
MFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLRQRV
HHHCCCCEEEEEEEECCEEEEEECCCCCEEECCHHHHHCCCEEEEEEECCCCHHHHHHHH
QEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQID
HHHCCCCEECEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
FSIHYNKFILRDPILHGVIDVIRNAF
EEEEECEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8444795; 8905232; 9278503 [H]