Click here to switch to the map view.

The map label for this gene is sucB

Identifier: 30062110

GI number: 30062110

Start: 590412

End: 591629

Strand: Reverse

Name: sucB

Synonym: S0583

Alternate gene names: 30062110

Gene position: 591629-590412 (Counterclockwise)

Preceding gene: 30062111

Following gene: 30062109

Centisome position: 12.86

GC content: 55.91

Gene sequence:

>1218_bases
ATGAGTAGCGTAGATATTCTGGTCCCTGACCTGCCTGAATCCGTAGCCGATGCCACCGTCGCAACCTGGCATAAAAAACC
CGGCGACGCAGTCGTACGTGATGAAGTGCTGGTAGAAATCGAAACTGACAAAGTGGTACTGGAAGTACCGGCATCAGCAG
ACGGCATTCTGGATGCGGTTCTGGAAGATGAAGGTACAACGGTAACGTCTCGTCAGATCCTTGGTCGCCTGCGTGAAGGC
AACAGCGCCGGTAAAGAAACCAGCGCCAAATCTGAAGAGAAAGCGTCCACTCCGGCGCAACGCCAGCAGGCGTCTCTGGA
AGAGCAAAACAACGATGCGTTAAGCCCGGCGATCCGTCGCCTGCTGGCTGAACACAATCTCGACGCCAGCGCCATTAAAG
GCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCG
GCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCG
TGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTA
TGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAA
GCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCGTCTATCGACGGCGATGACGTGGTTTACCACAACTATTT
CGACGTCAGCATGGCGGTTTCTACGCCGCGCGGCCTGGTGACGCCGGTTCTGCGTGATGTCGATACCCTCGGCATGGCAG
ACATCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGATGGCAAGCTGACGGTTGAAGATCTGACCGGTGGTAAC
TTCACCATCACCAACGGTGGTGTGTTCGGTTCCCTGATGTCTACGCCGATCATCAACCCGCCGCAGAGCGCAATTCTGGG
TATGCACGCTATCAAAGATCGTCCGATGGCGGTGAATGGTCAGGTTGAGATCCTGCCGATGATGTACCTGGCGCTGTCCT
ACGATCACCGTCTGATCGATGGTCGCGAATCCGTGGGCTTCCTGGTAACGATCAAAGAGTTACTGGAAGAGCCGACGCGT
CTGCTGCTGGACGTGTAG

Upstream 100 bases:

>100_bases
CCTCCGCCTCTCCGGCGGTAGGGTATATGTCCGTTCACCAGAAACAGCAACAAGATCTGGTTAATGACGCGCTGAACGTC
GAATAAATAAAGGATACACA

Downstream 100 bases:

>100_bases
TAGTTTAAGCTTCACCTGCACTGTAGACCGACGTGGATAGGGTGTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGG
ATGGACAGAACACATGAACT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREG
NSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGN
FTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEEPTR
LLLDV

Sequences:

>Translated_405_residues
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREG
NSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGN
FTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEEPTR
LLLDV
>Mature_404_residues
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGN
SAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPA
AAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVKA
VVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNF
TITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEEPTRL
LLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=57.6, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=28.476821192053, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=28.476821192053, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI31711992, Length=298, Percent_Identity=32.8859060402685, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=27.0207852193995, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=34.5238095238095, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=99.7530864197531, Blast_Score=817, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=406, Percent_Identity=30.5418719211823, Blast_Score=164, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=399, Percent_Identity=42.3558897243108, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=444, Percent_Identity=31.5315315315315, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25.4761904761905, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=31.0897435897436, Blast_Score=141, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=43.2098765432099, Blast_Score=321, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=27.3942093541203, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24645909, Length=235, Percent_Identity=59.1489361702128, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=28.5046728971963, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44026; Mature: 43895

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR
HCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVP
HHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCC
MTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIK
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
ELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNG
HHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECC
QVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEEPTRLLLDV
CEEHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDKVVLEVPASADGILDAV
CCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEEEEEEECCEEEEECCCCCCHHHHHH
LEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQQASLEEQNNDALSPAIRR
HCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHH
LLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAPAAAAPAAQPALAARSEKRVP
HHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCC
MTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRKQYGEAFEKRHGIRLGFMSFYVK
HHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHH
AVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTPRGLVTPVLRDVDTLGMADIEKKIK
HHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHH
ELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVNG
HHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECC
QVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEEPTRLLLDV
CEEHHHHHHHHHHCCCCCCCCCCCCCHHEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]