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The map label for this gene is ybaK

Identifier: 30061981

GI number: 30061981

Start: 442825

End: 443304

Strand: Reverse

Name: ybaK

Synonym: S0433

Alternate gene names: 30061981

Gene position: 443304-442825 (Counterclockwise)

Preceding gene: 30061982

Following gene: 30061979

Centisome position: 9.64

GC content: 50.83

Gene sequence:

>480_bases
ATGACACCCGCAGTTAAATTACTCGAAAAAAACAAGATTTCGTTTCAAATCCATACCTACGAGCACGATCCGGCTGAAAC
CAATTTTGGCGATGAAGTCGTCAAAAAATTAGGTTTGAATCCGGATCAGGTCTACAAAACGCTGCTGGTGGCAGTGAACG
GTGATATGAAACACCTTGCCGTGGCCGTTACGCCGGTCGCAGGTCAACTGGATCTTAAAAAAGTAGCAAAAGCGCTGGGT
GCCAAGAAAGTTGAGATGGCCGATCCGATGGTCGCGCAGCGTTCGACGGGATACCTGGTTGGGGGGATTAGCCCACTGGG
GCAGAAAAAACGTCTGCCAACGATTATCGACGCCCCCGCACAAGAATTTGCCACTATTTATGTTTCCGGCGGCAAGCGCG
GACTGGATATCGAACTGGCGGCAGGCGATCTGGCAAAGATCCTCGATGCCAAATTTGCTGATATCGCCCGCCGCGATTAA

Upstream 100 bases:

>100_bases
CACCATCCTGCCAATACTCTTAGGCAAGATGACTCTTTTTTTTGGACCGCCATCAGGCGTATGCTTGCTTCGTTTTTTTG
TTTAAGGCAGGAAAATTACC

Downstream 100 bases:

>100_bases
GCATTGTGCCGGATGCAAACATCCGGCACTTTCGGATTACTGCCAGCTCACCTCACCTTTCGGTTCATAAACACTCACAT
CCAGCGGCGAGCTTTTCTGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQVYKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALG
AKKVEMADPMVAQRSTGYLVGGISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD

Sequences:

>Translated_159_residues
MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQVYKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALG
AKKVEMADPMVAQRSTGYLVGGISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD
>Mature_158_residues
TPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQVYKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGA
KKVEMADPMVAQRSTGYLVGGISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD

Specific function: Functions in trans to edit the amino acid from incorrectly charged Cys-tRNA(Pro). May compensate for the lack of Cys-tRNA(Pro) editing by ProRS. Additionally serves as a general deacylase with weak activity against Cys-tRNA(Cys) and Gly- tRNA(Gly), as wel

COG id: COG2606

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prolyl-tRNA editing family. ProX subfamily

Homologues:

Organism=Escherichia coli, GI1786688, Length=159, Percent_Identity=100, Blast_Score=319, Evalue=6e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBAK_ECOLI (P0AAR3)

Other databases:

- EMBL:   U82664
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X03895
- PIR:   H64778
- RefSeq:   AP_001130.1
- RefSeq:   NP_415014.1
- PDB:   2DXA
- PDBsum:   2DXA
- ProteinModelPortal:   P0AAR3
- SMR:   P0AAR3
- DIP:   DIP-48133N
- IntAct:   P0AAR3
- MINT:   MINT-1228144
- STRING:   P0AAR3
- EnsemblBacteria:   EBESCT00000003221
- EnsemblBacteria:   EBESCT00000016125
- GeneID:   947083
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0470
- KEGG:   eco:b0481
- EchoBASE:   EB2348
- EcoGene:   EG12454
- eggNOG:   COG2606
- GeneTree:   EBGT00050000011115
- HOGENOM:   HBG727700
- OMA:   VSPLGQK
- ProtClustDB:   PRK10670
- BioCyc:   EcoCyc:EG12454-MONOMER
- Genevestigator:   P0AAR3
- GO:   GO:0005737
- GO:   GO:0006350
- InterPro:   IPR004369
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.90.960.10
- TIGRFAMs:   TIGR00011

Pfam domain/function: PF04073 YbaK; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: NA

Molecular weight: Translated: 17093; Mature: 16962

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQVYKTLLVAVNGDMKHLA
CCCHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEE
VAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGGISPLGQKKRLPTIIDAPA
EEEECCCCCCCHHHHHHHHCCCCCHHCCCHHHCCCCCEEEECCCCCCCCCCCCEEECCCC
QEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD
CCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQVYKTLLVAVNGDMKHLA
CCHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEE
VAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGGISPLGQKKRLPTIIDAPA
EEEECCCCCCCHHHHHHHHCCCCCHHCCCHHHCCCCCEEEECCCCCCCCCCCCEEECCCC
QEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD
CCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3012467; 10493123