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The map label for this gene is insF1 [C]

Identifier: 30061878

GI number: 30061878

Start: 329548

End: 329988

Strand: Reverse

Name: insF1 [C]

Synonym: S0326

Alternate gene names: 30061878

Gene position: 329988-329548 (Counterclockwise)

Preceding gene: 30061879

Following gene: 30061875

Centisome position: 7.17

GC content: 49.43

Gene sequence:

>441_bases
TTGTACCTCGCTGGCATCAAAGATGTTTATACGTGCGAAATTGTCGGCTACGCCATGGGAGAGCGCATGACAAAAGAGCT
GACAGGTAAAGCCCTGTTTATGGCGCTCAGGAGCCAGCGCCCACCTGCCGGGCTAATCCACCACTCTGATCGAGGTTCAC
AGTACTGCGCATACGATTACCGGGTCATACAGGAGCAGTTTGGTCTGAAAACATCAATGTCGCGTAAAGGTAACTGTTAC
GACAACGCTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTTAATAACCGGGATGA
AGCCATCTCAGTAATACGGGAATACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCTGGGGAATATCTCCC
CGGCAGCCTTCAGGGAAAAATATCATCAGATGGCTGCTTAA

Upstream 100 bases:

>100_bases
GCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTACAGCACCAAATCAGGTCTGGGTGGCGGACCTGACGTATG
TTGCCACACAGGAGGGATGG

Downstream 100 bases:

>100_bases
AAAAGAACAAATGGTAGTGTCCGCTATTGCCAGTACACCTCAACTTGTGAAGGAAATGGTACTCAGGGGGATTCCAGTAC
AGGTGGTGCGCAATTAGCTA

Product: IS600 orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCY
DNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Sequences:

>Translated_146_residues
MYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCY
DNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
>Mature_146_residues
MYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCY
DNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788405, Length=140, Percent_Identity=42.8571428571429, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787263, Length=140, Percent_Identity=42.8571428571429, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1786753, Length=140, Percent_Identity=42.8571428571429, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1786570, Length=140, Percent_Identity=42.8571428571429, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1786490, Length=140, Percent_Identity=42.8571428571429, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1789981, Length=140, Percent_Identity=36.4285714285714, Blast_Score=97, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 17023; Mature: 17023

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
RVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFY
HHHHHHHCCHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NRQRRHSRLGNISPAAFREKYHQMAA
HHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
RVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFY
HHHHHHHCCHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
NRQRRHSRLGNISPAAFREKYHQMAA
HHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]