Click here to switch to the map view.

The map label for this gene is leuA

Identifier: 30061639

GI number: 30061639

Start: 79286

End: 80857

Strand: Reverse

Name: leuA

Synonym: S0071

Alternate gene names: 30061639

Gene position: 80857-79286 (Counterclockwise)

Preceding gene: 30061640

Following gene: 161486552

Centisome position: 1.76

GC content: 52.74

Gene sequence:

>1572_bases
ATGAGCCAGCAAGTCATTATTTTCGATACCACATTGCGCGACGGTGAACAGGCGTTACAGGCAAGCTTGAGTGTGAAAGA
AAAACTGCAAATTGCGCTGGCCCTTGAGCGTATGGGTGTTGACGTGATGGAAGTCGGTTTCCCCGTCTCTTCGCCGGGCG
ATTTTGAATCGGTGCAAACCATCGCCCGCCAGGTTAAAAACAGCCGCGTATGTGCGTTAGCTCGCTGCGTGGAAAAAGAT
ATCGACGTGGCGGCCGAATCCCTGAAAGTCGCCGAAGCCTTCCGTATTCATACCTTTATTGCTACCTCCCCAATGCACAT
CGCCACCAAGCTGCGCAGCACCCTAGACGAGGTGATCGAACGCGCTATCTATATGGTGAAACGCGCCCGTAATTACACCG
ATGATGTTGAATTTTCTTGCGAAGATGCCGGGCGTACACCCATTGCCGATCTGGCGCGAGTGGTCGAAGCGGCGATTAAT
GCCGGTGCCACCACCATCAACATTCCGGACACCGTGGGCTACACCATGCCGTTTGAGTTCGCCGGAATCATCAGCGGCCT
GTATGAACGCGTACCTAACATCGACAAAGCCATTATTTCCGTACATACCCACGACGATTTAGGCCTGGCGGTCGGCAACT
CACTGGCGGCGGTACATGCCGGTGCACGCCAGGTGGAAGGTGCAATGAACGGGATCGGCGAGCGTGCCGGTAACTGTTCG
CTGGAAGAAGTCATCATGGCGATTAAAGTTCGTAAGGATATTCTCAACGTCCACACCGCCATTAATCACCAAGAGATATG
GCGCACCAGCCAGTTAGTTAGCCAGATTTGTAATATGCCGATCCCGGCAAACAAAGCCATTGTTGGCAGCGGCGCATTCG
CTCACTCCTCCGGTATCCACCAGGATGGCGTGCTGAAAAACCGCGAAAACTACGAAATCATGACACCAGAATCTATTGGT
CTGAACCAAATCCAGCTGAATCTGACCTCTCGTTCGGGGCGTGCGGCGGTGAAACATCGCATGGATGAGATGGGGTATAA
AGAAAGTGAATATAATTTAGACAATTTGTACGATGCTTTCCTGAAGCTGGCGGACAAAAAAGGTCAGGTGTTTGATTACG
ATCTGGAGGCGCTGGCCTTCATCGGTAAGCAGCAAGAAGAGCCGGAGCATTTCCGTCTGGATTACTTCAGCGTGCAGTCT
GGCTCTAACGATATCGCCACCGCCGCCGTCAAACTGGCCTGTGGCGAAGAAGTCAAAGCAGAAGCCGCCAACGGTAACGG
TCCGGTCGATGCCGTCTATCAGGCAATTAACCGCATCACTGACTATAACGTCGAACTGGTGAAATACAGCCTGACCGCCA
AAGGTCACGGTAAAGATGCGCTGGGTCAGGTGGATATTGTCACCAACTACAACGGTCGCCGCTTCCACGGCGTCGGCCTG
GCCACCGATATTGTCGAGTCCTCCGCCAAAGCCATGGTGCACGTACTGAACAATATCTGGCGTGCCGCAGAAGTCGAAAA
AGAGTTGCAACGCAAAACTCAACACAACGAAAACAACAAGGAAACCGTGTGA

Upstream 100 bases:

>100_bases
AGCATTAAGCCAGCACGCAGTCAAACAAAAAACCCGCGCCATTGCGCGGGTTTTTTTATGCCCGAAGCGAGGCGCTCTAA
AAGAGACAAGGACCCAAACC

Downstream 100 bases:

>100_bases
TGTCGAAGAATTACCATATTGCCGTATTGCCGGGAGACGGTATTGGTCCGGAAGTGATGACCCAGGCGCTGAAAGTGCTG
AATGCCGTGCGCAACCGCTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKD
IDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIG
LNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQS
GSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVTNYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKTQHNENNKETV

Sequences:

>Translated_523_residues
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKD
IDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIG
LNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQS
GSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVTNYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKTQHNENNKETV
>Mature_522_residues
SQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDI
DVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINA
GATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCSL
EEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGL
NQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSG
SNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVTNYNGRRFHGVGLA
TDIVESSAKAMVHVLNNIWRAAEVEKELQRKTQHNENNKETV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=523, Percent_Identity=99.4263862332696, Blast_Score=1076, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320019, Length=359, Percent_Identity=27.5766016713092, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=359, Percent_Identity=27.2980501392758, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=24.8161764705882, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324225, Length=541, Percent_Identity=24.2144177449168, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 57345; Mature: 57213

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
AGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAF
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKA
HHHHCCCCCEEECCHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHC
EAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVTNYNGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCCCEEECCCH
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKTQHNENNKETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHH
IARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIATSPMHIATKLRSTLDEVIE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAINAGATTINIPDTVGYTMPFEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
AGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAVHAGARQVEGAMNGIGERAGNCS
HHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQICNMPIPANKAIVGSGAFAHSSGIH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGRAAVKHRMDEMGYKESEYNLDNLYDAF
CCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LKLADKKGQVFDYDLEALAFIGKQQEEPEHFRLDYFSVQSGSNDIATAAVKLACGEEVKA
HHHHCCCCCEEECCHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHC
EAANGNGPVDAVYQAINRITDYNVELVKYSLTAKGHGKDALGQVDIVTNYNGRRFHGVGL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEECCCCCEEECCCH
ATDIVESSAKAMVHVLNNIWRAAEVEKELQRKTQHNENNKETV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA