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The map label for this gene is thiP

Identifier: 30061632

GI number: 30061632

Start: 70239

End: 71849

Strand: Reverse

Name: thiP

Synonym: S0064

Alternate gene names: 30061632

Gene position: 71849-70239 (Counterclockwise)

Preceding gene: 30061633

Following gene: 30061631

Centisome position: 1.56

GC content: 58.54

Gene sequence:

>1611_bases
ATGGCAACGCGCCGTCAGCCGTTAATTCCCGGCTGGTTAATTCCAGGTGTAAGCGCCGCCACGCTGGTGGTAGCGGTTGC
GCTCGCGGCGTTTCTCGCCCTGTGGTGGAACGCGCCGCAGGGTAACTGGGTGGCAGTCTGGCAGGACAGCTACCTGTGGC
ATGTGGTGCGCTTCTCCTTCTGGCAGGCGTTTCTCTCGGCGCTACTCTCTGTCGTACCCGCGATATTCCTCGCCCGCGCC
CTCTATCGCAGGCGCTTTCCGGGTCGGCTGGCGCTGTTGCGTCTGTGCGCAATGACCTTGATCCTCCCGGTGCTAGTTGC
TGTTTTCGGCATTCTTAGCGTCTATGGTCGTCAGGGCTGGCTGGCATCGCTCTGCCAATCGCTCGGTCTGGAGTGGACCT
TTTCGCCCTACGGCCTGCAAGGCATTTTGCTGGCGCACGTATTTTTTAATCTGCCGATGGCGAGCCGCTTATTACTCCAG
GCACTGGAAAACATTCCCGGCGAACAACGTCAGCTTGCCGCCCAGCTTGGGATGCGCGGCTGGCATTTTTTCCGCTTCGT
CGAATGGCCGTGGCTACGGCGACAAATCCCGCCGGTTGCCGCGCTTATCTTTATGCTCTGTTTCGCCAGCTTCGCCACCG
TGCTATCGCTGGGCGGCGGTCCGCAGGCAACCACTATCGAGCTGGCTATTTATCAGGCGCTGAGTTACGACTACGATCCT
GCCCGCGCGGCGATGCTGGCGCTGCTCCAGATGGTGTGTTGCCTTGGGCTGGTGCTGTTGAGTCAGCGACTAAGTAAGGC
CATTGCGCCCGGCACCACGCTGCTGCAAGGCTGGCGCGATCCGGACGATCGTCTGCATAGCCGCATTTGCGACACGGTGT
TAATAGTGCTGGCGCTGCTGCTGTTGCTGCCACCGTTGCTGGCGGTGATCGTCGATGGGGTAAATCGCCAGTTGCCGGAA
GTGCTGGCACAACCGGTGCTGTGGCAGGCGCTGTGGACCTCGTTGCGTATTGCGCTGGCGGCAGGTGTATTGTGCGTAGT
GCTGACCATGATGCTGCTATGGAGCAGTCGCGAACTGCGGGCGCGGCAGAAAATGCTGGCGGGTCAGGCGCTGGAGATGA
GCGGCATGTTGATCCTCGCCATGCCGGGGATTGTGCTGGCTACCGGCTTCTTTTTACTGCTCAACAACACTATCGGCCTG
CCACAATCTGCTGACGGCATTGTGATTTTCACCAATGCGTTAATGGCGATCCCTTATGCGCTGAAAGTACTGGAAAACCC
GATGCGCGATATCACCGCCCGCTACAGTATGTTGTGTCAGTCGCTGGGCATTGAAGGCTGGTCGCGCTTAAAAGTGGTCG
AGCTGCGCGCCCTGAAACGTCCACTGGCGCAGGCGCTGGCTTTTGCCTGCGTGCTGTCGATTGGTGATTTTGGCGTAGTG
GCGTTGTTCGGTAACGATGATTTCCGTACCCTGCCGTTTTATCTCTACCAGCAAATTGGCTCCTATCGCAGCCAGGACGG
CGCGGTCACCGCGTTAATTCTGCTACTACTCTGTTTTCTGCTGTTTACCGTGATTGAAAAACTACCGGGGCGAAATGTTA
AAACTGACTGA

Upstream 100 bases:

>100_bases
TCACGCTGCCTGCCGGGTTTGAACAATTGACCAAACCGGCCACCACGCTGGAGTTCACACCAGCTGAAGTGGCGGCACAA
CGTCAGGCATGGATTAGCGA

Downstream 100 bases:

>100_bases
TATCACCTGGCTTTATCACCATTTGCCGATGCGTTTTAGCTTAACGGTAGAACGCGGCGAGCAGGTGGCAATCCTCGGGC
CAAGCGGCGCGGGTAAAAGT

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWVAVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARA
LYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
ARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPE
VLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGL
PQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFTVIEKLPGRNVKTD

Sequences:

>Translated_536_residues
MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWVAVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARA
LYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
ARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPE
VLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGL
PQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFTVIEKLPGRNVKTD
>Mature_535_residues
ATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWVAVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARAL
YRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQA
LENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDPA
RAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEV
LAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLP
QSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVVA
LFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFTVIEKLPGRNVKTD

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=536, Percent_Identity=98.6940298507463, Blast_Score=1046, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 59359; Mature: 59228

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWVAVWQDSYLWHVVRFSF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
WQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQALENIPGEQRQLAAQLGMRG
HHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCH
WHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCH
ARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTVLIVLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLPQSADGIVIFTNALMAIPYA
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHH
LKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFTVIEKLPGRNVKTD
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWVAVWQDSYLWHVVRFSF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHH
WQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLILPVLVAVFGILSVYGRQGW
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQALENIPGEQRQLAAQLGMRG
HHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCH
WHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLGGGPQATTIELAIYQALSYDYDP
HHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCH
ARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQGWRDPDDRLHSRICDTVLIVLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTSLRIALAAGVLCVVLTMMLLWSSRELR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARQKMLAGQALEMSGMLILAMPGIVLATGFFLLLNNTIGLPQSADGIVIFTNALMAIPYA
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHH
LKVLENPMRDITARYSMLCQSLGIEGWSRLKVVELRALKRPLAQALAFACVLSIGDFGVV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALFGNDDFRTLPFYLYQQIGSYRSQDGAVTALILLLLCFLLFTVIEKLPGRNVKTD
EEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]