| Definition | Shigella flexneri 2a str. 2457T, complete genome. |
|---|---|
| Accession | NC_004741 |
| Length | 4,599,354 |
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The map label for this gene is caiD
Identifier: 30061604
GI number: 30061604
Start: 34293
End: 35186
Strand: Reverse
Name: caiD
Synonym: S0035
Alternate gene names: 30061604
Gene position: 35186-34293 (Counterclockwise)
Preceding gene: 30061605
Following gene: 30061603
Centisome position: 0.77
GC content: 52.35
Gene sequence:
>894_bases ATGAAACGTCAGGGAACTACCCTGCCAGCTAATAATCACGCCCTAAAACAATATGCATTTTTTGCAGGGATGTTGTCGTC CCTAAAAAAGCAAAAATGGAGAAAAGGAATGAGTGAATCATTACATCTGACCCGCAATGGATCAATTCTGGAAATTACCC TTGATCGCCCAAAAGCGAATGCTATTGATGCAAAAACCAGCTTTGAAATGGGCGAAGTATTTCTAAATTTCCGTGACGAT CCGCAATTACGTGTCGCCATTATTACCGGTGCCGGAGAGAAATTCTTTTCCGCGGGCTGGGATTTAAAAGCGGCAGCAGA AGGCGAAGCACCGGATGCTGACTTTGGCCCGGGTGGTTTTGCGGGATTAACCGAAATTTTCAATCTCGACAAACCAGTTA TCGCAGCTGTGAACGGCTACGCCTTTGGCGGCGGTTTCGAACTGGCGCTGGCGGCAGATTTTATTGTTTGTGCCGATAAC GCCAGCTTCGCCCTGCCGGAAGCCAAACTGGGCATCGTTCCTGACAGCGGCGGTGTGCTGCGTCTGCCGAAGATCCTGCC GCCTACCATCGTCAATGAAATGGTGATGACAGGCAGACGAATGGGCGCAGAAGAGGCGCTGCGTTGGGGGATAGTCAACC GCGTGGTTAGCCAGGCGGAACTGATGGATAACGCCCGCGAACTGGCTCAGCAGCTGGTTAACAGCGCCCCGCTGGCGATT GCGGCGCTGAAAGAGATCTACCGCACCACCAGCGAAATGCCGGTAGAAGAAGCGTATCGCTATATTCGCAGCGGCGTGTT GAAACACTATCCATCGGTTCTGCATTCGGAAGATGCCATTGAAGGGCCGCTGGCGTTTGCCGAGAAGCGCGATCCGGTGT GGAAAGGACGTTAA
Upstream 100 bases:
>100_bases ACAAAATATGGCGAAATTTAAAGTGCCCTCTTATCTGGAGATCAGAAAAGATCTGCCACGTAATTGCTCGGGGAAAATAA TTAGAAAGAATCTGAAATAA
Downstream 100 bases:
>100_bases CAACCGTGAGCTATTACGCCTTTGAGGGGTTAATTCCGGTGGTTCACCCGACGGCGTTTGTCCATCCCAGTGCCGTCTTG ATTGGCGATGTGATTGTGGG
Product: carnitinyl-CoA dehydratase
Products: L-Carnitine.; L-carnitinyl-CoA [C]
Alternate protein names: Crotonobetainyl-CoA hydratase [H]
Number of amino acids: Translated: 297; Mature: 297
Protein sequence:
>297_residues MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADN ASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI AALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR
Sequences:
>Translated_297_residues MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADN ASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI AALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR >Mature_297_residues MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADN ASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI AALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR
Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]
COG id: COG1024
COG function: function code I; Enoyl-CoA hydratase/carnithine racemase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]
Homologues:
Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=33.8289962825279, Blast_Score=117, Evalue=9e-27, Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=30.1204819277108, Blast_Score=96, Evalue=4e-20, Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=34.2245989304813, Blast_Score=91, Evalue=2e-18, Organism=Homo sapiens, GI37594471, Length=241, Percent_Identity=29.045643153527, Blast_Score=88, Evalue=8e-18, Organism=Homo sapiens, GI37594469, Length=227, Percent_Identity=29.0748898678414, Blast_Score=88, Evalue=9e-18, Organism=Homo sapiens, GI4502327, Length=231, Percent_Identity=32.9004329004329, Blast_Score=84, Evalue=1e-16, Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=29.5275590551181, Blast_Score=82, Evalue=4e-16, Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=9e-14, Organism=Homo sapiens, GI45643119, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=1e-13, Organism=Homo sapiens, GI260275230, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=1e-13, Organism=Homo sapiens, GI20127408, Length=173, Percent_Identity=27.1676300578035, Blast_Score=73, Evalue=3e-13, Organism=Homo sapiens, GI157694516, Length=218, Percent_Identity=26.1467889908257, Blast_Score=72, Evalue=7e-13, Organism=Homo sapiens, GI213417737, Length=218, Percent_Identity=26.1467889908257, Blast_Score=71, Evalue=9e-13, Organism=Homo sapiens, GI221307494, Length=136, Percent_Identity=27.9411764705882, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI221136756, Length=132, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI221136753, Length=132, Percent_Identity=28.7878787878788, Blast_Score=67, Evalue=2e-11, Organism=Homo sapiens, GI157694520, Length=182, Percent_Identity=28.021978021978, Blast_Score=67, Evalue=3e-11, Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=99.6168582375479, Blast_Score=528, Evalue=1e-151, Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=36.3636363636364, Blast_Score=141, Evalue=5e-35, Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=35.3535353535354, Blast_Score=107, Evalue=1e-24, Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=31.651376146789, Blast_Score=101, Evalue=5e-23, Organism=Escherichia coli, GI1788597, Length=200, Percent_Identity=32, Blast_Score=93, Evalue=2e-20, Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=29.5019157088123, Blast_Score=92, Evalue=4e-20, Organism=Escherichia coli, GI1790281, Length=171, Percent_Identity=32.1637426900585, Blast_Score=90, Evalue=2e-19, Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=34.1085271317829, Blast_Score=141, Evalue=4e-34, Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=31.9838056680162, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=28.5140562248996, Blast_Score=119, Evalue=2e-27, Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=31.3131313131313, Blast_Score=101, Evalue=6e-22, Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=27.6264591439689, Blast_Score=99, Evalue=4e-21, Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=25.2830188679245, Blast_Score=92, Evalue=2e-19, Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=1e-18, Organism=Caenorhabditis elegans, GI17558304, Length=173, Percent_Identity=30.0578034682081, Blast_Score=88, Evalue=6e-18, Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=28.8537549407115, Blast_Score=86, Evalue=2e-17, Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=28.1818181818182, Blast_Score=83, Evalue=2e-16, Organism=Caenorhabditis elegans, GI25144276, Length=186, Percent_Identity=29.5698924731183, Blast_Score=79, Evalue=3e-15, Organism=Caenorhabditis elegans, GI17508953, Length=187, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=4e-15, Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=29.5698924731183, Blast_Score=78, Evalue=5e-15, Organism=Caenorhabditis elegans, GI25144157, Length=246, Percent_Identity=26.8292682926829, Blast_Score=71, Evalue=8e-13, Organism=Caenorhabditis elegans, GI25144160, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=1e-12, Organism=Saccharomyces cerevisiae, GI6320241, Length=184, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=2e-11, Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=35, Blast_Score=138, Evalue=3e-33, Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=35, Blast_Score=138, Evalue=3e-33, Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=35.6617647058824, Blast_Score=127, Evalue=1e-29, Organism=Drosophila melanogaster, GI19922422, Length=289, Percent_Identity=28.719723183391, Blast_Score=103, Evalue=1e-22, Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=30.9090909090909, Blast_Score=96, Evalue=2e-20, Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=28.2608695652174, Blast_Score=92, Evalue=5e-19, Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=3e-17, Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=3e-16, Organism=Drosophila melanogaster, GI28571730, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=7e-13, Organism=Drosophila melanogaster, GI28571729, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=7e-13, Organism=Drosophila melanogaster, GI19921000, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI24583077, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-12, Organism=Drosophila melanogaster, GI24583079, Length=186, Percent_Identity=30.1075268817204, Blast_Score=69, Evalue=4e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022852 - InterPro: IPR014748 - InterPro: IPR001753 - InterPro: IPR018376 [H]
Pfam domain/function: PF00378 ECH [H]
EC number: 4.2.1.- [C]
Molecular weight: Translated: 32339; Mature: 32339
Theoretical pI: Translated: 6.54; Mature: 6.54
Prosite motif: PS00166 ENOYL_COA_HYDRATASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 3.0 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKAN CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCEEEEEECCCCCC AIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGF CCCCHHHHHHHHHEEECCCCCCEEEEEEECCCHHHHHCCCCCEECCCCCCCCCCCCCCCH AGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVL HHHHHHHCCCCCCEEEECCEEECCCEEEEEEEEEEEEECCCCEECCCCCEEEECCCCCEE RLPKILPPTIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH AALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCCCCCCC >Mature Secondary Structure MKRQGTTLPANNHALKQYAFFAGMLSSLKKQKWRKGMSESLHLTRNGSILEITLDRPKAN CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEECCCCEEEEEECCCCCC AIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGF CCCCHHHHHHHHHEEECCCCCCEEEEEEECCCHHHHHCCCCCEECCCCCCCCCCCCCCCH AGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVL HHHHHHHCCCCCCEEEECCEEECCCEEEEEEEEEEEEECCCCEECCCCCEEEECCCCCEE RLPKILPPTIVNEMVMTGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAI ECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH AALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: D-Carnitine; D-carnitinyl-CoA [C]
Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]
General reaction: Isomerases [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: NA