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The map label for this gene is miaA

Identifier: 29349731

GI number: 29349731

Start: 5694197

End: 5695117

Strand: Reverse

Name: miaA

Synonym: BT_4323

Alternate gene names: 29349731

Gene position: 5695117-5694197 (Counterclockwise)

Preceding gene: 29349732

Following gene: 29349730

Centisome position: 90.97

GC content: 47.12

Gene sequence:

>921_bases
ATGCCGGACTATGATTTAATCACCATATTAGGGCCAACAGCCTCAGGGAAAACTCCTTTTGCTGCAGCATTAGCCCATGA
ACTGAATACAGAAATTATCAGTGCCGACTCTCGTCAGATCTATCGGGGAATGGACCTCGGTACGGGCAAGGACTTGGCAG
ATTATACAGTCGACGGACATCCAATTCCTTATCACTTGATTGATATCGCCGATCCCGGATATAAATACAATGTTTTCGAA
TATCAGCGGGATTTCCTGATTTCTTACGAATCCATCAAACAAAAAGGCTGTCTGCCCGTTTTATGTGGAGGGACAGGTAT
GTATCTGGAATCGGTACTGAAAGGCTATAAGCTGATGCCGGTACCGGAAAATCCGGAGTTACGTGCCCGTCTGGCTAATC
ACTCTCTCGAAGAACTGACCGAAATCCTGAAGCAATATAAAACATTGCACAACTCTACGGATGTAGATACCGTGAAACGT
GCAATCCGTGCGATAGAAATTGAGGAGTATTATGCCGTGCATCCGGTTCCCGAACGGGAGTTTCCGAAGTTGAACAGCCT
CATCATCGGGGTAGATATCGATCGGGAACTGCGTCGCGAGAAGATCACCCGACGGTTGAAACAACGGCTGGACGAAGGAA
TGGTGGATGAAGTAAGGCGATTAACAGAACAAGGCATTTCACCGGACGACTTGATTTATTACGGACTGGAATACAAATTC
CTGACGTTATACGTCATAGGCAAACTGACTTACGAAGAGATGTTCACTGAGCTGGAGACGGCCATCCATCAGTTCGCCAA
ACGTCAGATGACTTGGTTCCGCGGCATGGAACGTCGGGGATTTACCATCCACTGGGTCAGTGCAGAGCTGCCTATGGAAG
AAAAGATAGCCTTTGTGATAGAAAAACTGCGAGGGAATTAG

Upstream 100 bases:

>100_bases
TTTGAGTTCTGCTTCCTATTAAATAATCAGATAAAAGCATCCAAATAACCCACTGGGTCAAAATTATTTTGTATTTTTGC
CGTCATTAAAGAATAAAGCT

Downstream 100 bases:

>100_bases
TATCTTTGCCAGTCATTTACGAATGGTCTGTGAAAGATAAAAAATGCGATTGTTAATACAGTTAGGATAGAAATACGGTT
ATGAGTGTAGAACCTGGAAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 2; DMAPP:tRNA dimethylallyltransferase 2; DMATase 2; Isopentenyl-diphosphate:tRNA isopentenyltransferase 2; IPP transferase 2; IPPT 2; IPTase 2

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPDYDLITILGPTASGKTPFAAALAHELNTEIISADSRQIYRGMDLGTGKDLADYTVDGHPIPYHLIDIADPGYKYNVFE
YQRDFLISYESIKQKGCLPVLCGGTGMYLESVLKGYKLMPVPENPELRARLANHSLEELTEILKQYKTLHNSTDVDTVKR
AIRAIEIEEYYAVHPVPEREFPKLNSLIIGVDIDRELRREKITRRLKQRLDEGMVDEVRRLTEQGISPDDLIYYGLEYKF
LTLYVIGKLTYEEMFTELETAIHQFAKRQMTWFRGMERRGFTIHWVSAELPMEEKIAFVIEKLRGN

Sequences:

>Translated_306_residues
MPDYDLITILGPTASGKTPFAAALAHELNTEIISADSRQIYRGMDLGTGKDLADYTVDGHPIPYHLIDIADPGYKYNVFE
YQRDFLISYESIKQKGCLPVLCGGTGMYLESVLKGYKLMPVPENPELRARLANHSLEELTEILKQYKTLHNSTDVDTVKR
AIRAIEIEEYYAVHPVPEREFPKLNSLIIGVDIDRELRREKITRRLKQRLDEGMVDEVRRLTEQGISPDDLIYYGLEYKF
LTLYVIGKLTYEEMFTELETAIHQFAKRQMTWFRGMERRGFTIHWVSAELPMEEKIAFVIEKLRGN
>Mature_305_residues
PDYDLITILGPTASGKTPFAAALAHELNTEIISADSRQIYRGMDLGTGKDLADYTVDGHPIPYHLIDIADPGYKYNVFEY
QRDFLISYESIKQKGCLPVLCGGTGMYLESVLKGYKLMPVPENPELRARLANHSLEELTEILKQYKTLHNSTDVDTVKRA
IRAIEIEEYYAVHPVPEREFPKLNSLIIGVDIDRELRREKITRRLKQRLDEGMVDEVRRLTEQGISPDDLIYYGLEYKFL
TLYVIGKLTYEEMFTELETAIHQFAKRQMTWFRGMERRGFTIHWVSAELPMEEKIAFVIEKLRGN

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=324, Percent_Identity=23.1481481481481, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1790613, Length=307, Percent_Identity=32.8990228013029, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25144712, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324848, Length=302, Percent_Identity=23.841059602649, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24649830, Length=108, Percent_Identity=36.1111111111111, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA2_BACTN (Q89ZQ3)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813234.1
- ProteinModelPortal:   Q89ZQ3
- SMR:   Q89ZQ3
- GeneID:   1073719
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4323
- NMPDR:   fig|226186.1.peg.4321
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- BioCyc:   BTHE226186:BT_4323-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35375; Mature: 35244

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDYDLITILGPTASGKTPFAAALAHELNTEIISADSRQIYRGMDLGTGKDLADYTVDGH
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHCCCCCC
PIPYHLIDIADPGYKYNVFEYQRDFLISYESIKQKGCLPVLCGGTGMYLESVLKGYKLMP
CCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCEEEC
VPENPELRARLANHSLEELTEILKQYKTLHNSTDVDTVKRAIRAIEIEEYYAVHPVPERE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCC
FPKLNSLIIGVDIDRELRREKITRRLKQRLDEGMVDEVRRLTEQGISPDDLIYYGLEYKF
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCHHE
LTLYVIGKLTYEEMFTELETAIHQFAKRQMTWFRGMERRGFTIHWVSAELPMEEKIAFVI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
EKLRGN
HHHHCC
>Mature Secondary Structure 
PDYDLITILGPTASGKTPFAAALAHELNTEIISADSRQIYRGMDLGTGKDLADYTVDGH
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHCCCCCC
PIPYHLIDIADPGYKYNVFEYQRDFLISYESIKQKGCLPVLCGGTGMYLESVLKGYKLMP
CCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCEEEC
VPENPELRARLANHSLEELTEILKQYKTLHNSTDVDTVKRAIRAIEIEEYYAVHPVPERE
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEECCCCCCC
FPKLNSLIIGVDIDRELRREKITRRLKQRLDEGMVDEVRRLTEQGISPDDLIYYGLEYKF
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCHHE
LTLYVIGKLTYEEMFTELETAIHQFAKRQMTWFRGMERRGFTIHWVSAELPMEEKIAFVI
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
EKLRGN
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928