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The map label for this gene is miaA

Identifier: 29349611

GI number: 29349611

Start: 5539855

End: 5540781

Strand: Reverse

Name: miaA

Synonym: BT_4203

Alternate gene names: 29349611

Gene position: 5540781-5539855 (Counterclockwise)

Preceding gene: 29349612

Following gene: 29349605

Centisome position: 88.51

GC content: 44.23

Gene sequence:

>927_bases
ATGCCCACTCTCATTGTTTTAATCGGACCTACAGGAGTAGGAAAAACAGAATTAAGCCTGCGACTGGCGGAGAACTTTCA
CACCAGCATTGTCTCCGCTGATTCGAGACAACTTTATGCTGAATTAAAAATAGGAACGGCGGCTCCTACCCCGGATCAAT
TAAAACGTGTGCCTCACTATCTGGTAGGCACTCTTCATCTTACCGATTATTACAGCGCCGCTCAATATGAGCAGGAAGCG
ATGGAAATCCTCCATCAATTATTCACAGAACATGAGGTAGTAGTACTTACTGGTGGATCAATGATGTATGTAGATGCCAT
CTGCAAGGGGATTGACGACATCCCTACCGTTGATGCTGAAACACGTCAGGTGATGCTGCAAAAGTATGAGGAAGAAGGAT
TGGAGCAACTTTGTGCAGAGCTCCGTTTATTAGATCCGGACTACTATCGCATCGTAGACCTGAAAAACCCAAAACGGGTG
ATTCATGCATTGGAAATCTGTTACATGACCGGAAAGACATATACATCTTTCCGCACCCAACAGAAAAAGGAACGCCCTTT
CCGCATTCTTAAGATCGGATTAACCCGTGACAGAGAAGAACTGTATGACCGCATTAATCGTCGTGTAGACCAAATGATGG
AGGAAGGATTACTGGACGAAGTACGTTCCGTACTCTCCTACCGGCATCTAAACTCTCTTAATACCGTAGGTTACAAAGAA
CTGTTCAAATATCTGGACGGAGAATGGGAACTCCCTTTCGCTATCGAAAAGATCAAGCAAAATTCACGCATTTATTCACG
CAAACAGATGACATGGTTCAAAAGGGATGAAGAAATACGCTGGTTCCATCCGGAACAAGAAACGGAGATACTTGAGTACC
TCCGTCTACAAAATCTGACTCATTTACCCAGTTTGGATACCTTCTGA

Upstream 100 bases:

>100_bases
TAGGTATTAATTGCTATGCAGTGACAAGCAGTTAATACCTAATATTTTTATCCCCTGGCACTTTAATACTTAACACCTAA
TACTTAATACTTTAAAATCC

Downstream 100 bases:

>100_bases
TAAACTTCCCGATAGACATTATTGCCCAACCGGAACAGAAGCGTCATTTTCTTTCCGTCAATCCGGTAAGATATAGGAAC
GATAGCATTTGCCGATCTGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 1; DMAPP:tRNA dimethylallyltransferase 1; DMATase 1; Isopentenyl-diphosphate:tRNA isopentenyltransferase 1; IPP transferase 1; IPPT 1; IPTase 1

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPTLIVLIGPTGVGKTELSLRLAENFHTSIVSADSRQLYAELKIGTAAPTPDQLKRVPHYLVGTLHLTDYYSAAQYEQEA
MEILHQLFTEHEVVVLTGGSMMYVDAICKGIDDIPTVDAETRQVMLQKYEEEGLEQLCAELRLLDPDYYRIVDLKNPKRV
IHALEICYMTGKTYTSFRTQQKKERPFRILKIGLTRDREELYDRINRRVDQMMEEGLLDEVRSVLSYRHLNSLNTVGYKE
LFKYLDGEWELPFAIEKIKQNSRIYSRKQMTWFKRDEEIRWFHPEQETEILEYLRLQNLTHLPSLDTF

Sequences:

>Translated_308_residues
MPTLIVLIGPTGVGKTELSLRLAENFHTSIVSADSRQLYAELKIGTAAPTPDQLKRVPHYLVGTLHLTDYYSAAQYEQEA
MEILHQLFTEHEVVVLTGGSMMYVDAICKGIDDIPTVDAETRQVMLQKYEEEGLEQLCAELRLLDPDYYRIVDLKNPKRV
IHALEICYMTGKTYTSFRTQQKKERPFRILKIGLTRDREELYDRINRRVDQMMEEGLLDEVRSVLSYRHLNSLNTVGYKE
LFKYLDGEWELPFAIEKIKQNSRIYSRKQMTWFKRDEEIRWFHPEQETEILEYLRLQNLTHLPSLDTF
>Mature_307_residues
PTLIVLIGPTGVGKTELSLRLAENFHTSIVSADSRQLYAELKIGTAAPTPDQLKRVPHYLVGTLHLTDYYSAAQYEQEAM
EILHQLFTEHEVVVLTGGSMMYVDAICKGIDDIPTVDAETRQVMLQKYEEEGLEQLCAELRLLDPDYYRIVDLKNPKRVI
HALEICYMTGKTYTSFRTQQKKERPFRILKIGLTRDREELYDRINRRVDQMMEEGLLDEVRSVLSYRHLNSLNTVGYKEL
FKYLDGEWELPFAIEKIKQNSRIYSRKQMTWFKRDEEIRWFHPEQETEILEYLRLQNLTHLPSLDTF

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=26.2987012987013, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1790613, Length=291, Percent_Identity=29.553264604811, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=270, Percent_Identity=22.5925925925926, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324848, Length=287, Percent_Identity=26.4808362369338, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24649830, Length=327, Percent_Identity=26.9113149847095, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA1_BACTN (Q8A018)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813114.1
- ProteinModelPortal:   Q8A018
- SMR:   Q8A018
- GeneID:   1074717
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4203
- NMPDR:   fig|226186.1.peg.4201
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q8A018
- ProtClustDB:   PRK00091
- BioCyc:   BTHE226186:BT_4203-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36188; Mature: 36057

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLIVLIGPTGVGKTELSLRLAENFHTSIVSADSRQLYAELKIGTAAPTPDQLKRVPHY
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHCHHH
LVGTLHLTDYYSAAQYEQEAMEILHQLFTEHEVVVLTGGSMMYVDAICKGIDDIPTVDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHH
TRQVMLQKYEEEGLEQLCAELRLLDPDYYRIVDLKNPKRVIHALEICYMTGKTYTSFRTQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHH
QKKERPFRILKIGLTRDREELYDRINRRVDQMMEEGLLDEVRSVLSYRHLNSLNTVGYKE
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LFKYLDGEWELPFAIEKIKQNSRIYSRKQMTWFKRDEEIRWFHPEQETEILEYLRLQNLT
HHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCC
HLPSLDTF
CCCCCCCC
>Mature Secondary Structure 
PTLIVLIGPTGVGKTELSLRLAENFHTSIVSADSRQLYAELKIGTAAPTPDQLKRVPHY
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCCCHHHHHHCHHH
LVGTLHLTDYYSAAQYEQEAMEILHQLFTEHEVVVLTGGSMMYVDAICKGIDDIPTVDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCCCCCHH
TRQVMLQKYEEEGLEQLCAELRLLDPDYYRIVDLKNPKRVIHALEICYMTGKTYTSFRTQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHH
QKKERPFRILKIGLTRDREELYDRINRRVDQMMEEGLLDEVRSVLSYRHLNSLNTVGYKE
HHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LFKYLDGEWELPFAIEKIKQNSRIYSRKQMTWFKRDEEIRWFHPEQETEILEYLRLQNLT
HHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCC
HLPSLDTF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928