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The map label for this gene is lipB

Identifier: 29346499

GI number: 29346499

Start: 1376273

End: 1376956

Strand: Reverse

Name: lipB

Synonym: BT_1089

Alternate gene names: 29346499

Gene position: 1376956-1376273 (Counterclockwise)

Preceding gene: 29346501

Following gene: 29346491

Centisome position: 21.99

GC content: 48.83

Gene sequence:

>684_bases
ATGAAAACTGTGTTAGTAGACTGGAATTTGATTCCGTACGCCGAGGCATGGCAGCGGCAGACGGAATGGTTTGATACTCT
TGTCCGCGCAAAAGCGCAGGGAGAAGCGTATGAAAACCGTATTATTATGTGCGAACATCCGCACGTCTATACTTTGGGAC
GTAGCGGAAAAGAAAATAATATGCTGTTGAATGACGATCAGCTGAAAGCCATTCAGGCTACCCTTTTCCATATAGATCGG
GGAGGGGATATCACGTATCACGGTCCCGGACAGCTAGTCTGTTATCCGATCTTGAATCTGGAAGAGTTTCATTTGGGGTT
GAAGGAATATGTGCACCTGCTCGAAGAGGCTGTGATACGTGTCTGTGCCTCCTATGGCATTGAAGCGGGACGGCTTGAAA
AGGCAACAGGTGTCTGGCTGGAGGGGAGTACGCCCCGTGCCCGTAAGATTTGTGCGATCGGGGTGAGGAGCAGCCACTAC
GTCACCATGCACGGACTGGCCTTGAATGTGAATACGGATTTACGCTATTTCAGCTATATTCATCCTTGTGGCTTTATAGA
TAAAGGCGTCACTTCTCTTCGTCAGGAACTGAAGCACGAAGTGCCTATGGACGAAGTGAAGCAACGCCTTGAATGCGAGC
TTGGCAGACTGCTGAACGAAAAAAAACAGCAGATTGCTCAATGA

Upstream 100 bases:

>100_bases
TCCATTTTCATAAGAGTGTTTGTTATCTTTGGTTTAGAGTGCTTGTTATATTCGATCTACAAATGTACGATTTTTCTTTT
TATTTCTTACTTTTGTCCTC

Downstream 100 bases:

>100_bases
AAGAACCCGGCAGACTCTGCTCAATGATAAAACTTAGCGGACTGCCCGAAGGAAAAGCCTAGAAAACGATTCCAATGCCG
AGCTGCCATGTCTTATGCAT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKTVLVDWNLIPYAEAWQRQTEWFDTLVRAKAQGEAYENRIIMCEHPHVYTLGRSGKENNMLLNDDQLKAIQATLFHIDR
GGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIRVCASYGIEAGRLEKATGVWLEGSTPRARKICAIGVRSSHY
VTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLRQELKHEVPMDEVKQRLECELGRLLNEKKQQIAQ

Sequences:

>Translated_227_residues
MKTVLVDWNLIPYAEAWQRQTEWFDTLVRAKAQGEAYENRIIMCEHPHVYTLGRSGKENNMLLNDDQLKAIQATLFHIDR
GGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIRVCASYGIEAGRLEKATGVWLEGSTPRARKICAIGVRSSHY
VTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLRQELKHEVPMDEVKQRLECELGRLLNEKKQQIAQ
>Mature_227_residues
MKTVLVDWNLIPYAEAWQRQTEWFDTLVRAKAQGEAYENRIIMCEHPHVYTLGRSGKENNMLLNDDQLKAIQATLFHIDR
GGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIRVCASYGIEAGRLEKATGVWLEGSTPRARKICAIGVRSSHY
VTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLRQELKHEVPMDEVKQRLECELGRLLNEKKQQIAQ

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=210, Percent_Identity=40, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI87081767, Length=218, Percent_Identity=36.697247706422, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323268, Length=178, Percent_Identity=29.7752808988764, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355483, Length=191, Percent_Identity=40.3141361256544, Blast_Score=146, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_BACTN (Q8A8S8)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_810002.1
- ProteinModelPortal:   Q8A8S8
- GeneID:   1074024
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_1089
- NMPDR:   fig|226186.1.peg.1089
- HOGENOM:   HBG716160
- OMA:   TEDHERP
- PhylomeDB:   Q8A8S8
- ProtClustDB:   PRK14348
- BioCyc:   BTHE226186:BT_1089-MONOMER
- BRENDA:   2.3.1.181
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 26007; Mature: 26007

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 183-183

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVLVDWNLIPYAEAWQRQTEWFDTLVRAKAQGEAYENRIIMCEHPHVYTLGRSGKENN
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCEEEECCCCCCCC
MLLNDDQLKAIQATLFHIDRGGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIR
EEECCHHHHHHHHHHHEEECCCEEEEECCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHH
VCASYGIEAGRLEKATGVWLEGSTPRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYI
HHHHCCCCCCCCCCCCEEEEECCCCCHHEEEEEECCCCCEEEEEEEEEEECCCHHHHHHH
HPCGFIDKGVTSLRQELKHEVPMDEVKQRLECELGRLLNEKKQQIAQ
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTVLVDWNLIPYAEAWQRQTEWFDTLVRAKAQGEAYENRIIMCEHPHVYTLGRSGKENN
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCEEEECCCCCCCC
MLLNDDQLKAIQATLFHIDRGGDITYHGPGQLVCYPILNLEEFHLGLKEYVHLLEEAVIR
EEECCHHHHHHHHHHHEEECCCEEEEECCCCEEEEEECCHHHHHCCHHHHHHHHHHHHHH
VCASYGIEAGRLEKATGVWLEGSTPRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYI
HHHHCCCCCCCCCCCCEEEEECCCCCHHEEEEEECCCCCEEEEEEEEEEECCCHHHHHHH
HPCGFIDKGVTSLRQELKHEVPMDEVKQRLECELGRLLNEKKQQIAQ
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928