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The map label for this gene is holD [H]

Identifier: 29144830

GI number: 29144830

Start: 4751758

End: 4752195

Strand: Direct

Name: holD [H]

Synonym: t4600

Alternate gene names: 29144830

Gene position: 4751758-4752195 (Clockwise)

Preceding gene: 29144828

Following gene: 29144831

Centisome position: 99.16

GC content: 59.59

Gene sequence:

>438_bases
ATGACATCCCGACGAGACTGGCAGTTACAGCAACTGGGTATTACGCAGTGGGCGCTGCGACGTCCTGGCGCGCTACAGGG
CGAGATTGCGATTTCCCTTCCCGCGCACGTTCGTCTGATCGTGGTTGCAGAGGAATTACCCGCGCTGAACGAGCCGCTAA
TGCGCGATATTTTACGCGCGTTGACCGTGAGTCCCGATCAGGTTTTACCGCTGACGCCTGAGCGCGTCGCCATGCTGCCG
CAGGGCAGCCGTTGTAATAGCTGGCGGTTAGGAACTGACGCGCCGCTCCAACTCGAAGGCGCTCAGGTCACGACGCCGGC
GTTTAATGAACTCCGGGTAAACCCGGCGGCACGCGCCGCACTATGGCAACAAATCTGCGAACATGAACACGATTTCTATC
CTCAGCACGACCGATCTCCCCGCAGCCTGGCAGATTGA

Upstream 100 bases:

>100_bases
AATGCTCCTTAATTCAGACTGGCGGCGATTATAGTAGTTTGATGGCGGAGATTCGACAGATTTGCTATATTTGCGCCCCT
GATTACAGGAGCGTTTCGCT

Downstream 100 bases:

>100_bases
GCAACGCGCTCATGCGTTTCCGTGGAGCGAAAAAACGTTTTTCGGCAACCAGGGCGAACGGTATCTCAACTTTAAGCTGA
CTGCTGATGACAGAATGGCC

Product: DNA polymerase III subunit psi

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTSRRDWQLQQLGITQWALRRPGALQGEIAISLPAHVRLIVVAEELPALNEPLMRDILRALTVSPDQVLPLTPERVAMLP
QGSRCNSWRLGTDAPLQLEGAQVTTPAFNELRVNPAARAALWQQICEHEHDFYPQHDRSPRSLAD

Sequences:

>Translated_145_residues
MTSRRDWQLQQLGITQWALRRPGALQGEIAISLPAHVRLIVVAEELPALNEPLMRDILRALTVSPDQVLPLTPERVAMLP
QGSRCNSWRLGTDAPLQLEGAQVTTPAFNELRVNPAARAALWQQICEHEHDFYPQHDRSPRSLAD
>Mature_144_residues
TSRRDWQLQQLGITQWALRRPGALQGEIAISLPAHVRLIVVAEELPALNEPLMRDILRALTVSPDQVLPLTPERVAMLPQ
GSRCNSWRLGTDAPLQLEGAQVTTPAFNELRVNPAARAALWQQICEHEHDFYPQHDRSPRSLAD

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The exact function of the psi subunit is unknown [H]

COG id: COG3050

COG function: function code L; DNA polymerase III, psi subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790831, Length=137, Percent_Identity=78.1021897810219, Blast_Score=230, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004615
- InterPro:   IPR018382
- ProDom:   PD032475 [H]

Pfam domain/function: PF03603 DNA_III_psi [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 16312; Mature: 16181

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRRDWQLQQLGITQWALRRPGALQGEIAISLPAHVRLIVVAEELPALNEPLMRDILRA
CCCCCCCHHHHHCCHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
LTVSPDQVLPLTPERVAMLPQGSRCNSWRLGTDAPLQLEGAQVTTPAFNELRVNPAARAA
HCCCCCCCCCCCHHHHEECCCCCCCCCEECCCCCCEEECCCEECCCCCCCEECCHHHHHH
LWQQICEHEHDFYPQHDRSPRSLAD
HHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSRRDWQLQQLGITQWALRRPGALQGEIAISLPAHVRLIVVAEELPALNEPLMRDILRA
CCCCCCHHHHHCCHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHH
LTVSPDQVLPLTPERVAMLPQGSRCNSWRLGTDAPLQLEGAQVTTPAFNELRVNPAARAA
HCCCCCCCCCCCHHHHEECCCCCCCCCEECCCCCCEEECCCEECCCCCCCEECCHHHHHH
LWQQICEHEHDFYPQHDRSPRSLAD
HHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8389364; 8366044; 2828880; 7610040; 9278503; 8505305; 1575709 [H]